Contrary to remarkable advances within the cell therapy industry,
scientists expose dissatisfied challenges associated with the
preservation and post-thaw cell death globally. Post cryopreservation
apoptosis is normally observed in cultures and scientists are focusing
on incorporation of apoptosis inhibitors. Impressive transport of cells
without affecting their survival and function is a crucial and pivotal
factor in any practical cell-based therapies. Preservation of cells
permits the transportation of cells between distances, as well as
improvement of safety and quality control testing in clinical and
research applications. The prosperity of transportation methods is
evaluated through the viability and proliferation percentages of the
transported cell. For many decades, the conventional methods of
transferring cells globally having adverse effects and speculated to be
a challenging and expensive method. The main purpose of some studies is
the optimization of cell survival after cryopreservation. In the new
generation of cryopreservation science, various experiments wish to
discover suitable and alternative methods for cell transportation to
ship viable cells at ambient temperature without dry ice or in media
filled flasks. In this review we try to represent a summary of the
detection of recent studies including dry preservation, hypothermic
preservation, agarose-gel based method, polymer based cryogel matrix,
encapsulation method, fibrin microbeads, osmolyte solution composition,
collagen-based scaffold, natural zwitterionic betaine, bio-inspired
cryo-ink that have been performed alternative, effective and economic
methods for shipping viable cells at ambient temperature.