Design and Immunological Properties of the Novel Subunit Virus-like Vaccine against SARS-CoV-2

Красильников, И В; Kudriavtsev, Aleksandr V.; Vakhrusheva, Anna; Frolova, Maria E.; Иванов, Александр Викторович; Stukova, Marina; Romanovskaya-Romanko, Ekaterina A.; Vasilyev, Kirill; Mushenkova, Nataliya V.; Isaev, Artur

doi:10.3390/vaccines10010069

Cited by 11 publications

(9 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result of the pandemic of the new coronavirus infection SARS-CoV-2, there is the demand for disinfecting agents and hand sanitizers based on Ag NPs due to their pronounced antiviral properties [20]. In addition, a COVID-19 vaccine containing spherical NPs as the carrier has been patented [21]. It is known that many synthesis technologies can be used for the relatively inexpensive commercial production of spherical Ag NPs.…”

Section: Uses Of Silvermentioning

confidence: 99%

Effect of Different Forms of Silver on Biological Objects

2022

View full text Add to dashboard Cite

Silver has been known since ancient times on account of its pronounced antiseptic properties. Currently, its antibacterial, antiviral, and fungicidal properties are highly desired in the food and cosmetic industries, in medicine, and pharmacology. Silver exhibits toxic effects not only on pathogenic organisms but also on healthy cells. Over the past 20 years, nanosilver, a new form of silver, has been introduced in various areas of industry. The transition to the nanoscale form results in the revision of standard approaches to items, including those based on this element, and the emergence of such a novel research area as nanosafety. In this review, we address the history of using different forms of silver, the mechanisms of its interaction with living cells, toxic properties, biokinetic parameters, capability for accumulation in different organs, effects on cognitive functions, and the clinically known argyrosis condition. Relevant publications are critically analyzed and conclusions are drawn. The broader incorporation of such a weakly biophilic element as silver in the biosphere and ecosphere calls for our understanding of biochemical processes underlying the interaction of this element, in its different forms, with living cells and multicellular organisms.

show abstract

Section: Uses Of Silvermentioning

confidence: 99%

Effect of Different Forms of Silver on Biological Objects

2022

View full text Add to dashboard Cite

show abstract

“…The study of immunogenic properties of the “Betuvax-CoV-2” vaccine has proved its ability to stimulate both humoral and cellular immune response. Previously we showed [7] that double immunization of mice led to the formation of an adaptive T-cell immune response. We observed an increase in both CD4+ and CD8+ Tem spike-specific cells after two administrations.…”

Section: Discussionmentioning

confidence: 99%

“…We have developed a subunit virus-like vaccine “Betuvax-CoV-2” against the SARS-CoV-2 based on the RBD and the SD1 domain of the S1 protein, fused with the human IgG1 Fc-fragment. This coronavirus antigen is being absorbed on the surface of betulin-based spherical particles 100–180 nm in size, mimicking the SARS-CoV-2 [7]. In the present paper, we have summarized the preclinical findings of the “Betuvax-CoV-2” vaccine on its safety, immunogenicity, and protectiveness.…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2 Subunit Virus-Like Vaccine Demonstrates High Safety Profile and Protective Efficacy: Preclinical Study

Vakhrusheva

Kudriavtsev

Kryuchkov

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Public health threat coming from a rapidly developing COVID-19 pandemic calls for developing safe and effective vaccines with innovative designs. This paper presents preclinical trial results of Betuvax-CoV-2, a vaccine developed as a subunit vaccine containing a recombinant RBD-Fc fusion protein and betulin-based spherical virus-like nanoparticles as an adjuvant (Betuspheres). The aim of the study was to demonstrate vaccine safety in mice, rats, and Chinchilla rabbits through acute, subchronic, and reproductive toxicity studies. Along with safety, the vaccine demonstrated protective efficacy through SARS-CoV-2 neutralizing antibody production in mice, rats, hamsters, rabbits, and primates (rhesus macaque), and lung damage and infection protection in hamsters and rhesus macaque model. Eventually, Betuvax-CoV-2 was proved to confer superior efficacy and protection against the SARS-CoV-2 in preclinical studies. Based on the above results, the vaccine was enabled to enter clinical trials that are currently underway.

show abstract

“…The antigen is expressed in CHO cells, adsorbed on a plant-derived adjuvant, betulin (pentacyclic triterpenoid) that forms spherical particles (betuspheres). The authors showed that the vaccine induced the production of high titers of antibodies (RBD-specific and SARS-CoV-2-neutralizing) and the development of T cell immunity in mice [ 133 ]. The Betuvax-CoV-2 vaccine is undergoing phase 1/2 clinical trials (NCT05270954).…”

Section: Recombinant Covid-19 Vaccinesmentioning

confidence: 99%

Recombinant Protein Vaccines against Human Betacoronaviruses: Strategies, Approaches and Progress

Kovalenko

Ryabchevskaya

Evtushenko

et al. 2023

IJMS

View full text Add to dashboard Cite

Betacoronaviruses have already troubled humanity more than once. In 2002–2003 and 2012, the SARS-CoV and MERS-CoV, respectively, caused outbreaks of respiratory syndromes with a fatal outcome. The spread of the SARS-CoV-2 coronavirus has become a pandemic. These three coronaviruses belong to the genus Betacoronavirus and have a zoonotic origin. The emergence of new coronavirus infections in the future cannot be ruled out, and vaccination is the main way to prevent the spread of the infection. Previous experience in the development of vaccines against SARS and MERS has helped to develop a number of vaccines against SARS-CoV-2 in a fairly short time. Among them, there are quite a few recombinant protein vaccines, which seem to be very promising in terms of safety, minimization of side effects, storage and transportation conditions. The problem of developing a universal betacoronavirus vaccine is also still relevant. Here, we summarize the information on the designing of vaccines based on recombinant proteins against highly pathogenic human betacoronaviruses SARS-CoV, MERS-CoV and SARS-CoV-2.

show abstract

Design and Immunological Properties of the Novel Subunit Virus-like Vaccine against SARS-CoV-2

Cited by 11 publications

References 46 publications

Effect of Different Forms of Silver on Biological Objects

Effect of Different Forms of Silver on Biological Objects

SARS-CoV-2 Subunit Virus-Like Vaccine Demonstrates High Safety Profile and Protective Efficacy: Preclinical Study

Recombinant Protein Vaccines against Human Betacoronaviruses: Strategies, Approaches and Progress

Contact Info

Product

Resources

About