Design and fabrication of PDMS microfluidics device for rapid and label-free DNA detection

Ayoib, A.; Hashim, U.; Gopinath, Subash C.B.; Thivina, V.; Arshad, M. K. Md

doi:10.1007/s00339-020-3337-7

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…The photolithography process was performed with some modifications to ensure high-quality fabrication [12]. Initially, the pattern transfer from the mask to the glass slide was accomplished to create the SU-8 master template.…”

Section: Fabrication Of Master Template Using Negative Photoresist Su-8mentioning

confidence: 99%

“…Polydimethylsiloxane (PDMS), a silicon-based bioorganic polymer, has gained attention as a suitable material for microfluidics fabrication due to its ability to produce devices quickly and inexpensively without requiring specialized clean room facilities [10]. PDMS offers several favorable properties, including biocompatibility, low cost, non-toxicity, thermo-tolerant, optical transparency, minimal autofluorescence, and the ability to be molded into complex shapes with high resolution [11], [12]. PDMS is also adaptable for integrating fluidic valves, establishing leakproof connections, detecting subtle forces like biomechanical interactions from cells and facilitating gas delivery for cell culture on microfluidics devices.…”

Section: Introductionmentioning

confidence: 99%

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Cost-Effective Fabrication of Polydimethylsiloxane (PDMS) Microfluidics for Point-of-Care Application

Adilah Ayoib,

Noor Amalina Aini Abdul Karim,

Uda Hashim

et al. 2024

IJNeaM

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Microfluidics fabrication pertains to the construction of small-scale devices and systems that manipulate and control small volumes of fluids. This process involves precise engineering and manufacturing procedures aimed at designing and producing these devices, which find applications in healthcare, environmental monitoring, and chemical analysis. The present study showcases an inexpensive approach to fabricate microfluidics channels using PDMS biopolymer and soft lithography technique to achieve laminar fluid flow. Initially, a robust and adhesive mold was created by fabricating a master template using several layers of SU-8 5 and SU-8 2015 negative photoresists. Subsequently, PDMS microfluidics channels were replicated and sealed onto a glass substrate through plasma bonding treatment. High-power microscopy images and profilometer analyses demonstrated successful fabrication with minimal deviation from the initial designs and the fabricated devices (less than 0.07 mm, less than 0.6°). Both the SU-8 master template and PDMS replicate displayed average microchannel height values and surface roughness of 100 μm and 0.26 μm or lower, respectively. Additionally, the fluid test confirmed laminar flow without any leakage post plasma oxidation, indicating the completion of an efficient and cost-effective fabrication process.

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Section: Fabrication Of Master Template Using Negative Photoresist Su-8mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cost-Effective Fabrication of Polydimethylsiloxane (PDMS) Microfluidics for Point-of-Care Application

Adilah Ayoib,

Noor Amalina Aini Abdul Karim,

Uda Hashim

et al. 2024

IJNeaM

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“…Current-voltage measurement results showed the accuracy of microfluidic devices for the real and synthesized samples of G. boninense . UV–vis studies and current-voltage measurements demonstrated effective DNA extraction ( Ayoib et al., 2020 ). With a pretreatment channel, inertial impactor, and low pressure collection chamber to enrich the airborne fungal spores, a microfluidic chip was created for the real-time monitoring of crop fungal disease.…”

Section: Modern Biosensor Technology For Plant Pathogen Detectionmentioning

confidence: 99%

A review of recent advances in plant-pathogen detection systems

Patel¹,

Mitra²,

Vinchurkar³

et al. 2022

Heliyon

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“…PDMS is one of the most preferred material in microfluidic biosensors [ 19 – 21 ]. Main reasons for its frequent use are its high biocompatibility, inexpensive, and non-toxic nature and exceptional fidelity of reproduction from micro-scale molding [ 22 ]. Other advantages include its ability to work well with aqueous media, high transparency, flexibility, and disallowance of non-specific adsorptions [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Pre-enrichment-free detection of hepatocellular carcinoma-specific ctDNA via PDMS and MEMS-based microfluidic sensor

Çağlayan Arslan,

Okan,

Külah

2024

Microchim Acta

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The growing interest in microfluidic biosensors has led to improvements in the analytical performance of various sensing mechanisms. Although various sensors can be integrated with microfluidics, electrochemical ones have been most commonly employed due to their ease of miniaturization, integration ability, and low cost, making them an established point-of-care diagnostic method. This concept can be easily adapted to the detection of biomarkers specific to certain cancer types. Pathological profiling of hepatocellular carcinoma (HCC) is heterogeneous and rather complex, and biopsy samples contain limited information regarding the tumor and do not reflect its heterogeneity. Circulating tumor DNAs (ctDNAs), which can contain information regarding cancer characteristics, have been studied tremendously since liquid biopsy emerged as a new diagnostic method. Recent improvements in the accuracy and sensitivity of ctDNA determination also paved the way for genotyping of somatic genomic alterations. In this study, three-electrode (Au-Pt–Ag) glass chips were fabricated and combined with polydimethylsiloxane (PDMS) microchannels to establish an electrochemical microfluidic sensor for detecting c.747G > T hotspot mutations in the TP53 gene of ctDNAs from HCC. The preparation and analysis times of the constructed sensor were as short as 2 h in total, and a relatively high flow rate of 30 µl/min was used during immobilization and hybridization steps. To the best of our knowledge, this is the first time a PDMS-based microfluidic electrochemical sensor has been developed to target HCC ctDNAs. The system exhibited a limit of detection (LOD) of 24.1 fM within the tested range of 2–200 fM. The sensor demonstrated high specificity in tests conducted with fully noncomplementary and one-base mismatched target sequences. The developed platform is promising for detecting HCC-specific ctDNA at very low concentrations without requiring pre-enrichment steps. Graphical Abstract

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Design and fabrication of PDMS microfluidics device for rapid and label-free DNA detection

Cited by 8 publications

References 22 publications

Cost-Effective Fabrication of Polydimethylsiloxane (PDMS) Microfluidics for Point-of-Care Application

Cost-Effective Fabrication of Polydimethylsiloxane (PDMS) Microfluidics for Point-of-Care Application

A review of recent advances in plant-pathogen detection systems

Pre-enrichment-free detection of hepatocellular carcinoma-specific ctDNA via PDMS and MEMS-based microfluidic sensor

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