Design and fabrication of Interdigitated Electrode (IDE) for detection of Ganoderma boninense

Thivina, V.; Hashim, U.; Arshad, M. K. Md; Ruslinda, A. Rahim; Ayoib, A.; Nordin, Norshahrizan

doi:10.1109/smelec.2016.7573588

Cited by 8 publications

(3 citation statements)

References 6 publications

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“…The difference was that they used quartz as a substrate instead of silicon. Therefore, these finding highlighted the importance of a substrate in the fabrication of functional electrodes [ 144 ].…”

Section: Micro and Nano Ideamentioning

confidence: 99%

Micro and Nano Interdigitated Electrode Array (IDEA)-Based MEMS/NEMS as Electrochemical Transducers: A Review

et al. 2022

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Micro and nano interdigitated electrode array (µ/n-IDEA) configurations are prominent working electrodes in the fabrication of electrochemical sensors/biosensors, as their design benefits sensor achievement. This paper reviews µ/n-IDEA as working electrodes in four-electrode electrochemical sensors in terms of two-dimensional (2D) planar IDEA and three-dimensional (3D) IDEA configurations using carbon or metal as the starting materials. In this regard, the enhancement of IDEAs-based biosensors focuses on controlling the width and gap measurements between the adjacent fingers and increases the IDEA’s height. Several distinctive methods used to expand the surface area of 3D IDEAs, such as a unique 3D IDEA design, integration of mesh, microchannel, vertically aligned carbon nanotubes (VACNT), and nanoparticles, are demonstrated and discussed. More notably, the conventional four-electrode system, consisting of reference and counter electrodes will be compared to the highly novel two-electrode system that adopts IDEA’s shape. Compared to the 2D planar IDEA, the expansion of the surface area in 3D IDEAs demonstrated significant changes in the performance of electrochemical sensors. Furthermore, the challenges faced by current IDEAs-based electrochemical biosensors and their potential solutions for future directions are presented herein.

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Section: Micro and Nano Ideamentioning

confidence: 99%

Micro and Nano Interdigitated Electrode Array (IDEA)-Based MEMS/NEMS as Electrochemical Transducers: A Review

et al. 2022

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“…Electrical biosensors, on the other hand, rely on the monitoring of changes in material electrical properties when bacteria bind in close proximity from the surface of, e.g., interdigital electrodes (IDE) designed on a solid substrate [ 11 , 12 ]. The signal response, often proportional to the number of bacteria, is used as an electrical fingerprint of the sample to provide fast, precise and quantified information about the bacteria presence in water.…”

Section: Introductionmentioning

confidence: 99%

Electrical Characterization of Cellulose-Based Membranes towards Pathogen Detection in Water

Brun¹,

Hauwaert²,

Leprince

et al. 2021

Biosensors

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Paper substrates are promising for development of cost-effective and efficient point-of-care biosensors, essential for public healthcare and environmental diagnostics in emergency situations. Most paper-based biosensors rely on the natural capillarity of paper to perform qualitative or semi-quantitative colorimetric detections. To achieve quantification and better sensitivity, technologies combining paper-based substrates and electrical detection are being developed. In this work, we demonstrate the potential of electrical measurements by means of a simple, parallel-plate electrode setup towards the detection of whole-cell bacteria captured in nitrocellulose (NC) membranes. Unlike current electrical sensors, which are mostly integrated, this plug and play system has reusable electrodes and enables simple and fast bacterial detection through impedance measurements. The characterized NC membrane was subjected to (i) a biofunctionalization, (ii) different saline solutions modelling real water samples, and (iii) bacterial suspensions of different concentrations. Bacterial detection was achieved in low conductivity buffers through both resistive and capacitive changes in the sensed medium. To capture Bacillus thuringiensis, the model microorganism used in this work, the endolysin cell-wall binding domain (CBD) of Deep-Blue, a bacteriophage targeting this bacterium, was integrated into the membranes as a recognition bio-interface. This experimental proof-of-concept illustrates the electrical detection of 107 colony-forming units (CFU) mL−1 bacteria in low-salinity buffers within 5 min, using a very simple setup. This offers perspectives for affordable pathogen sensors that can easily be reconfigured for different bacteria. Water quality testing is a particularly interesting application since it requires frequent testing, especially in emergency situations.

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“…To this end, novel ways of designing electronic elements on paper substrates, e.g., by printing common sensors such as interdigital electrodes (IDE), are being explored towards integrated devices with new functionalities [11][12][13]. IDE are typically used in applications such as moisture monitoring, chemical sensing, and biosensing [14,15].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Characterization of Nitrocellulose Membranes towards Bacterial Detection in Water

Brun¹,

Hauwaert²,

Leprince

et al. 2020

The 1st International Electronic Conference on Biosensors

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Paper substrates have shown a high potential for development of cost-effective and efficient point-of-care biosensors, essential for public healthcare and environmental diagnostics. Most paper-based biosensors rely on qualitative colorimetric detection schemes with high limits of detection. To overcome this limitation, technologies that combine paper-based substrates and electrochemical detection are being developed to allow for quantification and achieve better performances. In this work, we explore the potential of dielectric measurements towards electrical detection of whole-cell bacteria in nitrocellulose membranes, a paper-derivative. Impedance spectroscopy was considered to characterize the membranes with and without Bacillus thuringiensis cells, used as model microorganism. To specifically target this bacterial strain, phage endolysin cell-wall binding domain (CBD) encoded by a bacteriophage targeting B. thuringiensis were prepared and integrated into the membranes as recognition biointerface. The fluid sample containing the bacteria is conducted in the membrane through passive capillarity, and the bacteria are specifically immobilized in the test zone. Resulting changes of the dielectric properties of the membrane are sensed through impedance changes, highlighting the contribution of ions in the bacterial detection mechanism. This experimental proof-of-concept illustrates the electrical detection of 108 CFU/mL bacteria in low-salinity buffers within 5 min.

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Design and fabrication of Interdigitated Electrode (IDE) for detection of Ganoderma boninense

Cited by 8 publications

References 6 publications

Micro and Nano Interdigitated Electrode Array (IDEA)-Based MEMS/NEMS as Electrochemical Transducers: A Review

Micro and Nano Interdigitated Electrode Array (IDEA)-Based MEMS/NEMS as Electrochemical Transducers: A Review

Electrical Characterization of Cellulose-Based Membranes towards Pathogen Detection in Water

Electrochemical Characterization of Nitrocellulose Membranes towards Bacterial Detection in Water

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