Design and fabrication of electrospun polycaprolactone/chitosan scaffolds for ligament regeneration

Saatcioglu, Elif; Ulag, Songul; Şahin, Ali; Yılmaz, Baran; Ekren, Nazmi; İnan, Ahmet Talat; Palacı, Yüksel; Üstündağ, Cem Bülent; Gündüz, Oğuzhan

doi:10.1016/j.eurpolymj.2021.110357

Cited by 16 publications

(9 citation statements)

References 30 publications

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“…In recent years, electrospun PCL/CS has received a great deal of attention for the preparation of nanofiber scaffolds (Saatcioglu et al, 2021). PCL/CS nanofibers loaded with tetracycline hydrochloride (TCH) (Ghazalian et al, 2022), azithromycin (Alimohammadi et al, 2022), and metformin (Zhu et al, 2020) and its wound dressing containing aloe vera (Yin and Xu, 2020), Melilotus officinalis (Shahrousvand et al, 2021), and Jaft (Hashemi et al, 2021) have been recently reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of polycaprolactone/chitosan nanofibers containing antibacterial agents of curcumin and ZnO nanoparticles for use as wound dressing

Mosallanezhad

Nazockdast

Ahmadi

et al. 2022

Front. Bioeng. Biotechnol.

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The potential of the nanoscale structure is utilized by electrospun nanofibers, which are promising materials for wound dressings. Here, we prepared wound dressings constituting polycaprolactone (PCL) and chitosan (CS). Curcumin (Cur) and zinc oxide nanoparticles (ZnO) as antibacterial agents were embedded in PCL/CS electrospun nanofibers and different properties including morphology, physicomechanical, interaction with water, antibacterial efficiency, and in vitro studies were investigated. SEM images confirmed the nanofibrous structure of samples with 100 ± 5 to 212 ± 25 nm in average diameter. Elemental analysis of nanofibers showed a good distribution of ZnO along nanofibers which not only caused decreasing in nanofiber diameter but also increased tensile strength of nanofibers up to 2.9 ± 0.5 MPa and with good elongation at break of 39 ± 2.9. ZnO nanoparticles also facilitated the interaction of nanofibers with water, and this led to the highest water vapor transition rate, which was equal to 0.28 ± 0.02 g cm−2 day−1. The sample containing 3 wt% Cur had the highest water uptake value (367 ± 15%) and the lowest water contact angle (78 ± 3.7°), although Cur has a hydrophobic nature. The release profile of Cur showed a two-stage release and the Peppas model predicted a non-fickian diffusion. Simultaneous incorporation of CS, ZnO, and Cur effectively inhibited bacterial growth. In addition, in vitro studies represented that high content of Cur decreases cell viability and cell attachment. The outcomes from the fabricated nanofibrous scaffolds demonstrated appropriate properties for application as a wound dressing.

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Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of polycaprolactone/chitosan nanofibers containing antibacterial agents of curcumin and ZnO nanoparticles for use as wound dressing

Mosallanezhad

Nazockdast

Ahmadi

et al. 2022

Front. Bioeng. Biotechnol.

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“…Adding 1 wt% chitosan to a 10 wt% PCL solution significantly increased tensile strength, but higher concentrations of chitosan led to decreased tensile strength. Higher chitosan content improved the elongation at the point of break [ 87 ]. Surucu and Sasmazel created coaxial electrospun scaffolds with PCL and chitosan.…”

Section: Discussionmentioning

confidence: 99%

The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions

Osanloo,

Noori,

Varaa

et al. 2024

BMC Complement Med Ther

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Aims Thymus plant is a very useful herbal medicine with various properties such as anti-inflammatory and antibacterial. Therefore, the properties of this plant have made this drug a suitable candidate for wound healing. In this study, hydroxypropyl methylcellulose (HPMC) gel containing Zataria multiflora volatile oil nanoemulsion (neZM) along with polycaprolactone/chitosan (PCL-CS) nanofibrous scaffold was used, and the effect of three experimental groups on the wound healing process was evaluated. The first group, HPMC gel containing neZM, the second group, PCL-CS nanofibers, and the third group, HPMC gel containing neZM and bandaged with PCL-CS nanofibers (PCL-CS/neZM). Wounds bandaged with common sterile gas were considered as control. Methods The nanoemulsion was synthesized by a spontaneous method and loaded into a hydroxypropyl methylcellulose (HPMC) gel. The DLS test investigated the size of these nanoemulsions. A PCL-CS nanofibrous scaffold was also synthesized by electrospinning method then SEM and contact angle tests investigated morphology and hydrophilicity/hydrophobicity of its surface. The animal study was performed on full-thickness skin wounds in rats, and the process of tissue regeneration in the experimental and control groups was evaluated by H&E and Masson's trichrome staining. Results The results showed that the nanoemulsion has a size of 225±9 nm and has an acceptable dispersion. The PCL-CS nanofibers synthesized by the electrospinning method also show non-beaded smooth fibers and due to the presence of chitosan with hydrophilic properties, have higher surface hydrophobicity than PCL fibers. The wound healing results show that the PCL-CS/neZM group significantly reduced the wound size compared to the other groups on the 7th, 14th, and 21st days. The histological results also show that the PCL-CS/neZM group could significantly reduce the parameters of edema, inflammation, and vascularity and increase the parameters of fibrosis, re-epithelialization, and collagen deposition compared to other groups on day 21. Conclusion The results of this study show that the PCL-CS/neZM treatment can effectively improve wound healing.

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“…This behavior is related to the results obtained in the degradation test. In addition, by varying the length of the polymeric chain of the chitosan contained in the membranes, an increase in amino and hydroxyl groups occurs, which, when interacting with a neutral medium (pH ≈ 7.4), could decrease the viability of fibroblasts due to the protonation of functional groups and increase in free radicals that cause oxidative stress in the cellular model [36,51].…”

Section: Cell Viabilitymentioning

confidence: 99%

Effect of Molecular Weight and Nanoarchitecture of Chitosan and Polycaprolactone Electrospun Membranes on Physicochemical and Hemocompatible Properties for Possible Wound Dressing

et al. 2021

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Tissue engineering has focused on the development of biomaterials that emulate the native extracellular matrix. Therefore, the purpose of this research was oriented to the development of nanofibrillar bilayer membranes composed of polycaprolactone with low and medium molecular weight chitosan, evaluating their physicochemical and biological properties. Two-bilayer membranes were developed by an electrospinning technique considering the effect of chitosan molecular weight and parameter changes in the technique. Subsequently, the membranes were evaluated by scanning electron microscopy, Fourier transform spectroscopy, stress tests, permeability, contact angle, hemolysis evaluation, and an MTT test. From the results, it was found that changes in the electrospinning parameters and the molecular weight of chitosan influence the formation, fiber orientation, and nanoarchitecture of the membranes. Likewise, it was evidenced that a higher molecular weight of chitosan in the bilayer membranes increases the stiffness and favors polar anchor points. This increased Young’s modulus, wettability, and permeability, which, in turn, influenced the reduction in the percentage of cell viability and hemolysis. It is concluded that the development of biomimetic bilayer nanofibrillar membranes modulate the physicochemical properties and improve the hemolytic behavior so they can be used as a hemocompatible biomaterial.

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Design and fabrication of electrospun polycaprolactone/chitosan scaffolds for ligament regeneration

Cited by 16 publications

References 30 publications

Fabrication and characterization of polycaprolactone/chitosan nanofibers containing antibacterial agents of curcumin and ZnO nanoparticles for use as wound dressing

Fabrication and characterization of polycaprolactone/chitosan nanofibers containing antibacterial agents of curcumin and ZnO nanoparticles for use as wound dressing

The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions

Effect of Molecular Weight and Nanoarchitecture of Chitosan and Polycaprolactone Electrospun Membranes on Physicochemical and Hemocompatible Properties for Possible Wound Dressing

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