Design and Fabrication of Concentration‐Gradient Generators with Two and Three Inlets in Microfluidic Chips

Hu, Zengliang; Chen, Xueye; Wang, Lei

doi:10.1002/ceat.201700287

Cited by 12 publications

(11 citation statements)

References 29 publications

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“…To examine the performance of the device, we used two colours of food dyes (please refer to ESI for dye preparation protocol). The concentration profile of the fabricated CGG is illustrated in Figure 7D which is similar to those reported by the literature [54] . Since the velocity in CGG devices is small [55] , surface roughness cannot impose problems on the binding of PDMS.…”

Section: Biological Applicationssupporting

confidence: 84%

“…This type of CGG is usually used for cancer cell cultures as these CGGs transfer more oxygen and nutrients to cells as they develop a convective mass flux. Among various tree-like CGGs proposed in the literature, we chose the S-shaped CGG design developed by Hu et al [54] . The authors used micromilling to fabricate the CGG on a polymethylmethacrylate (PMMA) substrate.…”

Section: Biological Applicationsmentioning

confidence: 99%

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Rapid Softlithography Using 3D‐Printed Molds

Bazaz

Kashaninejad

Azadi

et al. 2019

Adv Materials Technologies

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most prevalent and conventional method for fabrication of PDMS-based microchips relies on softlithography, the main drawback of which is the preparation of a master mold which is costly and time-consuming. To prevent the attachment of PDMS to the master mold, silanization is necessary which can be detrimental for cellular studies. Also, using cell-compatible surfactant for mold coating adds extra pre-processing time. Recent advances in 3D printing have shown great promise in expediting the microfabrication process. Nevertheless, current 3D printing techniques are suboptimal for PDMS softlithography. In this paper, using a newly developed 3D printing resin, we have demonstrated the feasibility of producing master molds suitable for rapid softlithography. Moreover, we have showcased the utility of this technique for a number of widely used applications such as concentration gradient generation, particle separation, cell culture (to show biocompatibility of the process) and fluid mixing. The present study can open new opportunities for biologists and scientists with minimum knowledge of microfabrication to build functional microfluidic devices for their basic and applied research.

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Section: Biological Applicationssupporting

confidence: 84%

Section: Biological Applicationsmentioning

confidence: 99%

Rapid Softlithography Using 3D‐Printed Molds

Bazaz

Kashaninejad

Azadi

et al. 2019

Adv Materials Technologies

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“…Furthermore, microfluidic systems can robustly reduce the volume of chemical reagents and precious drugs because of the microscale size of microfluidic model. Microfluidic chips can provide an excellent platform to precisely control the concentration gradients of drug candidates, for studying how cellular phenotypes respond to the chemical and physical signals in the simulative extracellular microenvironment. ,, In the past few years, biochemical concentration gradients generated and precisely controlled by microfluidic systems have been widely used in the field of drug screening, including bacterial chemotaxis, cancer cell migration, and axon growth. − …”

Section: Main Advantages Of Microfluidic Cell-based Platforms For Dru...mentioning

confidence: 99%

“…Compared with conventional systems for drug screening, microfluidic platforms can dramatically reduce the volumes of high-cost compounds and save the costs of drug discovery. Concentration gradient generator on a microchip provides an excellent platform to evaluate the toxicity and the optimal concentration of different drugs in a large scale and quantity (Figure A). , Single-cell chips have great significance to monitor the single cell responses to chemical compounds, producing greater statistical information than conventional methods by small numbers of cells (Figure B). For the evaluation of drug candidates in vitro, the microenvironment in Petri dish is far from physiological conditions, while microfluidic devices can construct microenvironment which is closer to the in vivo physiological conditions by coculturing different cells in a natural way (Figure C). , Presently, many researchers rely on animal experiments.…”

mentioning

confidence: 99%

Cell-Based Assays on Microfluidics for Drug Screening

2019

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Microfluidics is an appealing platform for drug screening and discovery. Compared with the conventional drug screening methods based on Petri dishes and experimental animals, microfluidic devices have many advantages including miniaturized size, ease-to-use, high sensitivity, and high throughput. More importantly, bioassays on microfluidics can avoid ethical issues which can be a big obstacle hindering the performance of the experiments on animals or human being. Furthermore, three-dimensional (3D) microchips can recapitulate various biochemical and biophysical conditions in vivo and mimic the natural microenvironment of the tissues/organs, providing versatile in vitro models for biomedical applications. In this Perspective, we will focus on the cell-based microfluidic assays for drug screening. Meanwhile, we also propose potential solutions for the difficulties in this field and discuss the prospects of microfluidics-based technologies for drug screening.

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“…The numerical simulation method has been widely used in the research and development of microfluidic chips [23]. By solving the governing equations of fluid dynamics, the flow field, solution concentration distribution, and particle trajectory within the microchannel can be simulated to optimize the chip structure [24,25]. By solving the thermodynamic equations to understand the temperature change of the microfluidic chip under electrothermal conditions, the experimental cost of chip fabrication can be reduced [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Design and Modeling of a Microfluidic Coral Polyps Culture Chip with Concentration and Temperature Gradients

Zhou

Chen

et al. 2022

Micromachines

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Traditional methods of cultivating polyps are costly and time-consuming. Microfluidic chip technology makes it possible to study coral polyps at the single-cell level, but most chips can only be analyzed for a single environmental variable. In this work, we addressed these issues by designing a microfluidic coral polyp culture chip with a multi-physical field for multivariable analyses and verifying the feasibility of the chip through numerical simulation. This chip used multiple serpentine structures to generate the concentration gradient and used a circuit to form the Joule effect for the temperature gradient. It could generate different temperature gradients at different voltages for studying the growth of polyps in different solutes or at different temperatures. The simulation of flow field and temperature showed that the solute and heat could be transferred evenly and efficiently in the chambers, and that the temperature of the chamber remained unchanged after 24 h of continuous heating. The thermal expansion of the microfluidic chip was low at the optimal culture temperature of coral polyps, which proves the feasibility of the use of the multivariable microfluidic model for polyp culture and provides a theoretical basis for the actual chip processing.

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Design and Fabrication of Concentration‐Gradient Generators with Two and Three Inlets in Microfluidic Chips

Cited by 12 publications

References 29 publications

Rapid Softlithography Using 3D‐Printed Molds

Rapid Softlithography Using 3D‐Printed Molds

Cell-Based Assays on Microfluidics for Drug Screening

Design and Modeling of a Microfluidic Coral Polyps Culture Chip with Concentration and Temperature Gradients

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