Design and fabrication of an electrochemical aptasensor using Au nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite for rapid and sensitive detection of Staphylococcus aureus

Applications of Nanocellulose/Nanocarbon Composites: Focus on Biotechnology and Medicine

“…Anticancer drugs (doxorubicin) [72,95,96] Anticancer and other drugs [16] Anticancer drugs (doxorubicin) [97] Carbon quantum dots: Anticancer drugs (temozolomide) [28] (Bio)sensors Electrochemical: cholesterol [98]; glucose and bacteria [110]; avian leucosis virus [111]; organic liquids [112] Electrochemical: ATP metabolites [102]; oxygen [84] Electrochemical: Biotin [66] Carbon black: Electrochemical aptasensor for S. aureus [32]; electrochemical sensor for H 2 O 2 [33] Piezoelectric: strain, human motion [63,99,113] Piezoresistance and thermoelectric-based: pressure and temperature [103]; pressure [17]; strain, human motion [90,91,114];…”

“…At the same time, this material had electrochemical properties promising for the detection of hydrogen peroxide [31]. An electrochemical aptasensor for detecting Staphylococcus aureus, e.g., in human blood serum, was designed as a nanocomposite of Au nanoparticles, carbon black nanoparticles and cellulose nanofibers, and was endowed with a thiolated specific S. aureus aptamer as a sensing element [32].…”

“…In addition, nanocellulose/nanocarbon composites are promising for biomedical applications, though these applications are less frequent than industrial applications. Biomedical applications include radical scavenging [34,92], photothermal ablation of pathogenic bacteria [93], photodynamic and combined chemophotothermal therapy against cancer [35,94], drug delivery [16,28,65,72,[95][96][97], biosensorics [31][32][33]63,66,71,91,[98][99][100][101][102][103][104], and particularly tissue engineering and wound dressings. Hybrid materials containing nanocellulose and nanocarbons stimulated the growth and osteogenic differentiation of human bone marrow mesenchymal stem cells [37,59].…”

Rapid electrochemical detection of Staphylococcus aureus based on screen-printed gold electrodes modified with cysteine and Guinea grass (Panicum maximum) peroxidase

“…Glassy carbon electrode 1x10 1 -1x10 6 60 (Wei, et al, 2014) Amperometry Salmonella Glassy carbon electrode 1x10 1 -1x10 5 50 (Liebana, et al, 2009) EIS Staphylococcus aureus Gold nanoparticles 1.2x10 1 -1.2x10 8 30 (Shahrokhian, et al, 2018) Amperometry Escherichia coli Screen-printed carbon electrode 1x10 2 -1x10 7 - (Lin, et al, 2008) CV To test the ability of our SPGE-cys-GGP electrode in real samples, we used a brand of commercial milk from a local store. The milk sample was previously spiked with H 2 O 2 1 mM and S. aureus at a concentration of 10 4 CFU/mL -1 and we registered the electrocatalytic reduction current of H 2 O 2 by GGP.…”

Electrochemical immunosensor based on an antibody-hierarchical mesoporous SiO2 for the detection of Staphylococcus aureus

“…4 These methods have been applied and approved; however, most methods are expensive and cannot meet the fast, accurate, and highsensitivity requirements owing to the time and labor requirements. 5 Therefore, a rapid, convenient, and sensitive detection method for the quantitative detection of S. aureus concentration is required to prevent infection. 6 Electrochemical biosensors have been increasingly used in detection methods owing to their specicity, sensitivity, rapidity, and reproducibility.…”