Design and fabrication of a smart sensor using in silico epitope mapping and electro-responsive imprinted polymer nanoparticles for determination of insulin levels in human plasma

García‐Cruz, Álvaro; Haq, Isma; Cowen, Todd; Masi, Sabrina Di; Trivedi, Samir; Alanazi, Kaseb; Piletska, Elena; Mujahid, Adnan; Piletsky, Sergey A.

doi:10.1016/j.bios.2020.112536

Cited by 39 publications

(23 citation statements)

References 31 publications

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“…SPEs, like thermocouples, exhibit a rough surface, which enables immobilization of the nanoMIPs between groves in the different graphite sheets by drop casting. While drop casting is traditionally used in combination with electrochemistry measurements, , the advantage of using thermal analysis is that it can monitor binding over the whole surface including the binder areas (a significant proportion of the electrode) which are not conductive.…”

Section: Resultsmentioning

confidence: 99%

Immobilization of Molecularly Imprinted Polymer Nanoparticles onto Surfaces Using Different Strategies: Evaluating the Influence of the Functionalized Interface on the Performance of a Thermal Assay for the Detection of the Cardiac Biomarker Troponin I

McClements

Tchekwagep

Strapazon

et al. 2021

ACS Appl. Mater. Interfaces

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We demonstrate that a novel functionalized interface, where molecularly imprinted polymer nanoparticles (nanoMIPs) are attached to screen-printed graphite electrodes (SPEs), can be utilized for the thermal detection of the cardiac biomarker troponin I (cTnI). The ultrasensitive detection of the unique protein cTnI can be utilized for the early diagnosis of myocardial infraction (i.e., heart attacks), resulting in considerably lower patient mortality and morbidity. Our developed platform presents an innovative route to develop accurate, low-cost, and disposable sensors for the diagnosis of cardiovascular diseases, specifically myocardial infraction. A reproducible and advantageous solid-phase approach was utilized to synthesize high-affinity nanoMIPs (average size = 71 nm) for cTnI, which served as synthetic receptors in a thermal sensing platform. To assess the performance and commercial potential of the sensor platform, various approaches were used to immobilize nanoMIPs onto thermocouples or SPEs: dip coating, drop casting, and a covalent approach relying on electrografting with an organic coupling reaction. Characterization of the nanoMIP-functionalized surfaces was performed with electrochemical impedance spectroscopy, atomic force microscopy, and scanning electron microscopy. Measurements from an in-house designed thermal setup revealed that covalent functionalization of nanoMIPs onto SPEs led to the most reproducible sensing capabilities. The proof of application was provided by measuring buffered solutions spiked with cTnI, which demonstrated that through monitoring changes in heat transfer at the solid–liquid interface, we can measure concentrations as low as 10 pg L–1, resulting in the most sensitive test of this type. Furthermore, preliminary data are presented for a prototype platform, which can detect cTnI with shorter measurement times and smaller sample volumes. The excellent sensor performance, versatility of the nanoMIPs, and reproducible and low-cost nature of the SPEs demonstrate that this sensor platform technology has a clear commercial route with high potential to contribute to sustainable healthcare.

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Section: Resultsmentioning

confidence: 99%

Immobilization of Molecularly Imprinted Polymer Nanoparticles onto Surfaces Using Different Strategies: Evaluating the Influence of the Functionalized Interface on the Performance of a Thermal Assay for the Detection of the Cardiac Biomarker Troponin I

McClements

Tchekwagep

Strapazon

et al. 2021

ACS Appl. Mater. Interfaces

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“…Several successful examples of nanoMIPs have been exploited in bioanalysis for the development of optical and electrochemical sensors [ 137 , 149 , 150 ]. The company MIP Diagnostics has developed the first commercial nanoMIPs against different SARS-CoV-2 variants, addressing the RBD of SARS-CoV-2.…”

Section: Mip-based Biomimetic Sensors For Sars-cov-2 Detectionmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Sensors for SARS-CoV-2: Where Are We Now?

Yarman

Kurbanoğlu

2022

Biomimetics

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Since the first reported case of COVID-19 in 2019 in China and the official declaration from the World Health Organization in March 2021 as a pandemic, fast and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has played a major role worldwide. For this reason, various methods have been developed, comprising reverse transcriptase-polymerase chain reaction (RT-PCR), immunoassays, clustered regularly interspaced short palindromic repeats (CRISPR), reverse transcription loop-mediated isothermal amplification (RT-LAMP), and bio(mimetic)sensors. Among the developed methods, RT-PCR is so far the gold standard. Herein, we give an overview of the MIP-based sensors utilized since the beginning of the pandemic.

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“…The LOD of 26 and 81 fM was obtained in buffer and human plasma, respectively. 72 Selection of the right peptide epitope can significantly improve the sensor performance. Lee et al compared three epitopes for preparing EIP targeting alpha-synuclein, the biomarker of the Parkinson's disease.…”

Section: Advances In Protein Sensing and Separationmentioning

confidence: 99%