Design and Fabrication of a Dielectrophoretic Cell Trap Array

Velmanickam, Logeeshan; Nawarathna, Dharmakeerthi

doi:10.25046/aj020110

Cited by 3 publications

(3 citation statements)

References 29 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We patterned cells as single-files between IDEs to apply electric fields (DC or AC) uniformly across all the cells in the sample. The details of the cell patterning can be found elsewhere [26]. To electroporate cells using DC electric fields, a DC pulse of 12 V (or electric field of 5 × 10 5 V m −1 ) was applied for about 1 ms.…”

Section: Methodsmentioning

confidence: 99%

“…To manufacture the microfluidics device, we used standard micro-fabrication methods such as photolithography, metal evaporation, lift-off and wafer dicing. The details of micro-fabrication protocols are reported elsewhere [26]. Briefly, the IDE electrodes were fabricated on a glass substrate, and the flow channel was fabricated in polydimethylsiloxane (PDMS) and bonded together using the traditional oxygen plasma bonding technique [26].…”

Section: Methodsmentioning

confidence: 99%

“…The details of micro-fabrication protocols are reported elsewhere [26]. Briefly, the IDE electrodes were fabricated on a glass substrate, and the flow channel was fabricated in polydimethylsiloxane (PDMS) and bonded together using the traditional oxygen plasma bonding technique [26]. The cell purification device had an inlet and an outlet (figure 3(c)).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Label-free purification of viable human T-lymphocyte cells from a mixture of viable and non-viable cells after transfection by electroporation

Jayasooriya

Nawarathna

2019

J. Phys. D: Appl. Phys.

Self Cite

View full text Add to dashboard Cite

Recent developments in chimeric antigen receptor (CAR) T-cell therapy for cancer have shown promising results. CAR T-cells are engineered mainly by AC or DC electroporation to express CAR molecules on their surfaces. Electroporation has detrimental effects on the majority of T-cells and causes cell death via apoptosis or necrosis. These non-viable cells have potentially harmful effects on other healthy T-cells. Because there are no unique biomarkers that pinpoint the apoptosis and necrosis of T-cells, currently available cell separation methods are incapable of purifying healthy T-cells after electroporation. To address this critical issue, we have used dielectrophoretic cell isolation in a simple microfluidics chip. Our results indicate that it is possible to purify cell samples after electroporation and achieve ~100% purity and >90% target cell recovery. Therefore, this work presents a viable solution to a critical need in CAR T-cell manufacturing.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Label-free purification of viable human T-lymphocyte cells from a mixture of viable and non-viable cells after transfection by electroporation

Jayasooriya

Nawarathna

2019

J. Phys. D: Appl. Phys.

Self Cite

View full text Add to dashboard Cite

show abstract

Label Free MicroRNA Biomarker Detection in Serum Samples for Diagnosis Applications at Point-of Care

Velmanickam

Pokharel

Lima

et al. 2019

2019 IEEE Research and Applications of Photonics in Defense Conference (RAPID)

View full text Add to dashboard Cite

Recent progress in microRNA detection using integrated electric fields and optical detection methods

Velmanickam,

Nawarathna

2024

Front. Lab. Chip. Technol.

Self Cite

View full text Add to dashboard Cite

Low-cost, highly-sensitivity, and minimally invasive tests for the detection and monitoring of life-threatening diseases and disorders can reduce the worldwide disease burden. Despite a number of interdisciplinary research efforts, there are still challenges remaining to be addressed, so clinically significant amounts of relevant biomarkers in body fluids can be detected with low assay cost, high sensitivity, and speed at point-of-care settings. Although the conventional proteomic technologies have shown promise, their ability to detect all levels of disease progression from early to advanced stages is limited to a limited number of diseases. One potential avenue for early diagnosis is microRNA (miRNA). Due to their upstream positions in regulatory cascades, blood-based miRNAs are sensitive biomarkers that are detectable earlier than those targeted by other methods. Therefore, miRNA is a promising diagnostic biomarker for many diseases, including those lacking optimal diagnostic tools. Electric fields have been utilized to develop various biomedical assays including cell separation, molecules detection and analysis. Recently, there has been a great interest in the utility of electric fields with optical detection methods, including fluorescence and surface plasmons toward biomarker detection. This mini review first summarizes the recent development of miRNA as a biomarker. Second, the utility of electric fields and their integration with fluorescence detection methods will be discussed. Next, recent studies that utilized electric fields and optical detection methods will be discussed. Finally, in conclusion, technology gaps and improvements needed to enable low-cost and sensitive biomarker detection in point-of-care settings will be discussed.

show abstract

Design and Fabrication of a Dielectrophoretic Cell Trap Array

Abstract: We present a design and fabrication of an integrated micro-fabricated dielectrophoretic (DEP)

Cited by 3 publications

References 29 publications

Label-free purification of viable human T-lymphocyte cells from a mixture of viable and non-viable cells after transfection by electroporation

Label-free purification of viable human T-lymphocyte cells from a mixture of viable and non-viable cells after transfection by electroporation

Label Free MicroRNA Biomarker Detection in Serum Samples for Diagnosis Applications at Point-of Care

Recent progress in microRNA detection using integrated electric fields and optical detection methods

Contact Info

Product

Resources

About