Design and fabrication method of bi-layered fibrous scaffold for cartilage regeneration

Dabasinskaite, Lauryna; Krugly, Edvinas; Baniukaitiene, Odeta; Čiužas, Darius; Martuzevičius, Dainius; Jankauskaitė, Lina; Malinauskas, Mantas; Ūsas, Arvydas

doi:10.1016/j.bej.2022.108413

Cited by 6 publications

(7 citation statements)

References 76 publications

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“…However, in cartilage regeneration, such factors as scaffolds' mechanical strength are crucial. Our previous study demonstrated good mechanical and other properties of this electrospun PCL scaffold [27]. Different proteins can facilitate hMDSC differentiation as well as subsequent chondrocyte growth.…”

Section: Discussionmentioning

confidence: 72%

“…Poly(e-caprolactone) (PCL) scaffolds were prepared as described previously [27]. Briefly, the scaffolds were fabricated of PCL fibers with the addition of cellulose acetate using the custom-made solution cryo-electrospinning setup [28].…”

Section: Scaffold Fabrication Functionalization and Protein Bindingmentioning

confidence: 99%

“…Briefly, the scaffolds were fabricated of PCL fibers with the addition of cellulose acetate using the custom-made solution cryo-electrospinning setup [28]. The scaffolds were posttreated to convert cellulose acetate to cellulose and to introduce functional groups for the binding of the growth factor [27]. The fabricated fibrous mats were cut into 0.5 g specimens and subjected to the solution of 0.05 M NaOH/water-ethanol at 25 • C for 24 h. Afterwards, the scaffolds were placed in a glass reactor containing water (20 ± 1 • C) and treated by bubbling O 3 from an ex situ generator (GL-3188A, Shenzhen Guanglei, Shenzhen, China) into the reactor at a mass flow of 400 mg/h.…”

Section: Scaffold Fabrication Functionalization and Protein Bindingmentioning

confidence: 99%

“…The fabricated fibrous mats were cut into 0.5 g specimens and subjected to the solution of 0.05 M NaOH/water-ethanol at 25 • C for 24 h. Afterwards, the scaffolds were placed in a glass reactor containing water (20 ± 1 • C) and treated by bubbling O 3 from an ex situ generator (GL-3188A, Shenzhen Guanglei, Shenzhen, China) into the reactor at a mass flow of 400 mg/h. Subsequently, the samples were stored in a vacuum dryer at 21 ± 2 • C for 12 h. The morphology, chemical composition, hydrophilicity, absorption, and thermal and mechanical properties of the scaffold were evaluated and described in previous research [27]. The scaffold featured a random fibrous structure with a mean fiber diameter of 9.3 ± 4.1 µm, and a pore size ranging from 50 to 300 µm, having an overall scaffold porosity of 90.7%.…”

Section: Scaffold Fabrication Functionalization and Protein Bindingmentioning

confidence: 99%

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Functionalized Electrospun Scaffold–Human-Muscle-Derived Stem Cell Construct Promotes In Vivo Neocartilage Formation

et al. 2022

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Polycaprolactone (PCL) is a non-cytotoxic, completely biodegradable biomaterial, ideal for cartilage tissue engineering. Despite drawbacks such as low hydrophilicity and lack of functional groups necessary for incorporating growth factors, it provides a proper environment for different cells, including stem cells. In our study, we aimed to improve properties of scaffolds for better cell adherence and cartilage regeneration. Thus, electrospun PCL–scaffolds were functionalized with ozone and loaded with TGF-β3. Together, human-muscle-derived stem cells (hMDSCs) were isolated and assessed for their phenotype and potential to differentiate into specific lineages. Then, hMDSCs were seeded on ozonated (O) and non-ozonated (“naïve” (NO)) scaffolds with or without protein and submitted for in vitro and in vivo experiments. In vitro studies showed that hMDSC and control cells (human chondrocyte) could be tracked for at least 14 days. We observed better proliferation of hMDSCs in O scaffolds compared to NO scaffolds from day 7 to day 28. Protein analysis revealed slightly higher expression of type II collagen (Coll2) on O scaffolds compared to NO on days 21 and 28. We detected more pronounced formation of glycosaminoglycans in the O scaffolds containing TGF-β3 and hMDSC compared to NO and scaffolds without TGF-β3 in in vivo animal experiments. Coll2-positive extracellular matrix was observed within O and NO scaffolds containing TGF-β3 and hMDSC for up to 8 weeks after implantation. These findings suggest that ozone-treated, TGF-β3-loaded scaffold with hMDSC is a promising tool in neocartilage formation.

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Section: Discussionmentioning

confidence: 72%

Section: Scaffold Fabrication Functionalization and Protein Bindingmentioning

confidence: 99%

Section: Scaffold Fabrication Functionalization and Protein Bindingmentioning

confidence: 99%

Section: Scaffold Fabrication Functionalization and Protein Bindingmentioning

confidence: 99%

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Functionalized Electrospun Scaffold–Human-Muscle-Derived Stem Cell Construct Promotes In Vivo Neocartilage Formation

et al. 2022

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“…In this study, we aimed to evaluate the formation of cartilage tissue using rabbit muscle-derived stem cells (rMDSCs) on electrospun bilayer PCL ozone-treated scaffolds with loaded TGFβ3. The latter has been developed and validated in vitro during our earlier investigations ( Dabasinskaite et al, 2022 ; Jankauskaite et al, 2022 ). First, we investigated the scaffold’s potential in vitro to provide and maintain a microenvironment for rMDSC proliferation and differentiation into chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

Cartilage regeneration using improved surface electrospun bilayer polycaprolactone scaffolds loaded with transforming growth factor-beta 3 and rabbit muscle-derived stem cells

Malinauskas

Jankauskaitė²,

Aukstikalne³

et al. 2022

Front. Bioeng. Biotechnol.

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Polycaprolactone (PCL) has recently received significant attention due to its mechanical strength, low immunogenicity, elasticity, and biodegradability. Therefore, it is perfectly suitable for cartilage tissue engineering. PCL is relatively hydrophobic in nature, so its hydrophilicity needs to be enhanced before its use in scaffolding. In our study, first, we aimed to improve the hydrophilicity properties after the network of the bilayer scaffold was formed by electrospinning. Electrospun bilayer PCL scaffolds were treated with ozone and further loaded with transforming growth factor-beta 3 (TGFβ3). In vitro studies were performed to determine the rabbit muscle-derived stem cells’ (rMDSCs) potential to differentiate into chondrocytes after the cells were seeded onto the scaffolds. Statistically significant results indicated that ozonated (O) scaffolds create a better environment for rMDSCs because collagen-II (Coll2) concentrations at day 21 were higher than non-ozonated (NO) scaffolds. In in vivo studies, we aimed to determine the cartilage regeneration outcomes by macroscopical and microscopical/histological evaluations at 3- and 6-month time-points. The Oswestry Arthroscopy Score (OAS) was the highest at both mentioned time-points using the scaffold loaded with TGFβ3 and rMDSCs. Evaluation of cartilage electromechanical quantitative parameters (QPs) showed significantly better results in cell-treated scaffolds at both 3 and 6 months. Safranin O staining indicated similar results as in macroscopical evaluations—cell-treated scaffolds revealed greater staining with safranin, although an empty defect also showed better results than non-cell-treated scaffolds. The scaffold with chondrocytes represented the best score when the scaffolds were evaluated with the Mankin histological grading scale. However, as in previous in vivo evaluations, cell-treated scaffolds showed better results than non-cell-treated scaffolds. In conclusion, we have investigated that an ozone-treated scaffold containing TGFβ3 with rMDSC is a proper combination and could be a promising scaffold for cartilage regeneration.

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Nanostructured Biopolymer‐Based Constructs for Cartilage Regeneration: Fabrication Techniques and Perspectives

Sharma,

Satapathy

2024

Macromolecular Bioscience

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The essential functions of cartilage, such as shock absorption and resilience, are hindered by its limited regenerative capacity. Although current therapies alleviate symptoms, novel strategies for cartilage regeneration are desperately needed. Recent developments in 3D constructs aim to address this challenge by mimicking the intrinsic characteristics of native cartilage using biocompatible materials, with a significant emphasis on both functionality and stability. Through fabrication methods like 3D printing and electrospinning, researchers are making progress in cartilage regeneration; nevertheless, it is still very difficult to translate these advances into clinical practice. The review emphasizes the importance of integrating various fabrication techniques to create stable 3D constructs. Meticulous design and material selection are required to achieve seamless cartilage integration and durability. The review outlines the need to address these challenges and focuses on the latest developments in the production of hybrid 3D constructs based on biodegradable and biocompatible polymers. Furthermore, it acknowledges the limitations of current research and provides perspectives on potential avenues for effectively regenerating cartilage defects in the future.This article is protected by copyright. All rights reserved

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Design and fabrication method of bi-layered fibrous scaffold for cartilage regeneration

Cited by 6 publications

References 76 publications

Functionalized Electrospun Scaffold–Human-Muscle-Derived Stem Cell Construct Promotes In Vivo Neocartilage Formation

Functionalized Electrospun Scaffold–Human-Muscle-Derived Stem Cell Construct Promotes In Vivo Neocartilage Formation

Cartilage regeneration using improved surface electrospun bilayer polycaprolactone scaffolds loaded with transforming growth factor-beta 3 and rabbit muscle-derived stem cells

Nanostructured Biopolymer‐Based Constructs for Cartilage Regeneration: Fabrication Techniques and Perspectives

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