Design and Fabrication a Microfluidic Device for Fetal Cells Dielectrophoretic Properties Characterization

Xu, Guolin; Chan, Marcus; Yang, Chun; Sukumar, Pavithra; Choolani, Mahesh; Ying, Jackie Y.

doi:10.1088/1742-6596/34/1/182

Cited by 9 publications

(8 citation statements)

References 6 publications

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“…DEP offers a great potential for fetal cell discrimination and isolation without the use of antibody‐antigen interaction. The crossover frequency of fetal NRBC, measured using a microfluidic device with interdigitated electrodes, was found to be 50 KHz when the cell suspending buffer conductivity is 15 mS/m . Borgatti et al.…”

Section: Applications Of Dep In Poc Testingmentioning

confidence: 99%

Dielectrophoresis: Applications and future outlook in point of care

2013

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Dielectrophoresis (DEP) is a label free, noninvasive, stand alone, rapid, and sensitive particle manipulation and characterization technique. Improvements in micro-electro-mechanical systems technology have enabled the biomedical applications of DEP over the past decades. By this way, integration of DEP into lab-on-a-chip systems has become achievable, creating a potential tool for point-of-care (POC) systems. DEP can be utilized in many different POC applications including early detection and prognosis of various cancer types, diagnosis of infectious diseases, blood cell analysis, and stem cell therapy. However, there are still some challenges to be resolved to have DEP-based devices available in POC market. Today, researchers have focused on these challenges to have this powerful theory as a solution for many POC applications. Here, DEP theory, cell modeling, and most common device structures are introduced briefly. Next, POC applications of DEP theory, such as cell (blood, cancer, stem, and fetal) and microorganism separation, manipulation, and enrichment for diagnosis and prognosis, are explained. Integration of DEP with other detection techniques to have more sensitive systems is summarized. Finally, future outlook for DEP-based systems are discussed with some challenges, which are currently preventing these systems to be a common tool for POC applications, and possible solutions.

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Section: Applications Of Dep In Poc Testingmentioning

confidence: 99%

Dielectrophoresis: Applications and future outlook in point of care

2013

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“…An attempt to study fetal cell properties using microfluidic devices was presented by Xu et al 76 wherein dielectrophoresis (DEP) was used to trap the fNRBCs and to determine the crossover frequency of fetal cells. DEP cell separation is possible because different types of cell in blood respond differently to an electrical field, depending on the frequency applied (Fig.…”

Section: Microfluidic Approaches To Isolate Fetal Nucleated Cellsmentioning

confidence: 99%

“…4 (A) Dielectrophoresis chip to isolate fNRBCs from sparse samples. 76 (B) DEPArray™ technology from Silicon Biosystems. 82 (Reproduced with permission from Silicon Biosystems Inc.).…”

Section: Commercial Entities In Nipdmentioning

confidence: 99%

Lab-on-a-chip technology: impacting non-invasive prenatal diagnostics (NIPD) through miniaturisation

et al. 2014

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This paper aims to provide a concise review of non-invasive prenatal diagnostics (NIPD) to the lab-on-a-chip and microfluidics community. Having a market of over one billion dollars to explore and a plethora of applications, NIPD requires greater attention from microfluidics researchers. In this review, a complete overview of conventional diagnostic procedures including invasive as well as non-invasive (fetal cells and cell-free fetal DNA) types are discussed. Special focus is given to reviewing the recent and past microfluidic approaches to NIPD, as well as various commercial entities in NIPD. This review concludes with future challenges and ethical considerations of the field.

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“…Simple interdigitated electrodes have also been used to isolate rare circulating cells . Becke et al reported the isolation of cancer cells from blood cells using an electroaffinity column containing an interdigitated microelectrode array.…”

Section: Various Approaches To Isolate Rare Cells Using Microfluidic mentioning

confidence: 99%

Microfluidic devices for the isolation of circulating rare cells: A focus on affinity‐based, dielectrophoresis, and hydrophoresis

Hyun

Jung

2013

Electrophoresis

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Circulating rare cells have attracted interest because they can be good indicators of various types of diseases. For example, enumeration of circulating tumor cells is used for cancer diagnosis and prognosis, while DNA analysis or enumeration of nucleated red blood cells is useful for prenatal diagnosis or hypoxic anemia, and that of circulating stem cells to diagnose cancer metastasis. Isolation of these cells and their downstream analyses can provide significant information such as the origin and characteristics of a disease. Novel approaches based on microfluidics have many advantages, including the continuous process and integration with other components for analysis. For these reasons, a variety of microfluidic devices have been developed to isolate and characterize rare cells. In this article, we review several microfluidic devices, with a focus on affinity-based isolation (e.g. antigen-antibody reaction) and label-free separation (DEP and hydrophoresis).

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Design and Fabrication a Microfluidic Device for Fetal Cells Dielectrophoretic Properties Characterization

Cited by 9 publications

References 6 publications

Dielectrophoresis: Applications and future outlook in point of care

Dielectrophoresis: Applications and future outlook in point of care

Lab-on-a-chip technology: impacting non-invasive prenatal diagnostics (NIPD) through miniaturisation

Microfluidic devices for the isolation of circulating rare cells: A focus on affinity‐based, dielectrophoresis, and hydrophoresis

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