Design and evaluation of mesenchymal stem cells seeded chitosan/glycosaminoglycans quaternary hydrogel scaffolds for wound healing applications

Soriano-Ruiz, José L.; Gálvez‐Martín, Patricia; López‐Ruiz, Elena; Suñer-Carbó, Joaquim; Calpena-Campmany, Ana C.; Marchal, Juan Antonio; Clares, Beatriz

doi:10.1016/j.ijpharm.2019.118632

Cited by 23 publications

(9 citation statements)

References 44 publications

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“…Chitosan-based hydrogels have been further modified or functionalized to increase the biological activities of encapsulated cells ( Dhillon et al, 2019 ). Since it can also provide analgesic effect and hemostatic activity, much current research focuses on its application in wound healing ( Xu H. et al, 2019 ; Soriano-Ruiz et al, 2019 ). For example, injectable thermo-sensitive hydrogel loaded MSCs from umbilical cord blood was found to successfully accelerate wound closure and support tissue remodeling and regeneration of skin appendages for cutaneous wound healing.…”

Section: Polysaccharide Hydrogelsmentioning

confidence: 99%

“…For example, injectable thermo-sensitive hydrogel loaded MSCs from umbilical cord blood was found to successfully accelerate wound closure and support tissue remodeling and regeneration of skin appendages for cutaneous wound healing. ( Xu Y. et al, 2019 ; Soriano-Ruiz et al, 2019 ). Furthermore, chitosan-based hydrogel encapsulating ESC-derived endothelial cells and VEGF induced robust cell retention and promoted neovascularization through vasculogenesis and angiogenesis ( Lee et al, 2015 ).…”

Section: Polysaccharide Hydrogelsmentioning

confidence: 99%

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Polysaccharide-Based Hydrogels for Microencapsulation of Stem Cells in Regenerative Medicine

Lee

Khoo

et al. 2021

Front. Bioeng. Biotechnol.

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Stem cell-based therapy appears as a promising strategy to induce regeneration of damaged and diseased tissues. However, low survival, poor engraftment and a lack of site-specificity are major drawbacks. Polysaccharide hydrogels can address these issues and offer several advantages as cell delivery vehicles. They have become very popular due to their unique properties such as high-water content, biocompatibility, biodegradability and flexibility. Polysaccharide polymers can be physically or chemically crosslinked to construct biomimetic hydrogels. Their resemblance to living tissues mimics the native three-dimensional extracellular matrix and supports stem cell survival, proliferation and differentiation. Given the intricate nature of communication between hydrogels and stem cells, understanding their interaction is crucial. Cells are incorporated with polysaccharide hydrogels using various microencapsulation techniques, allowing generation of more relevant models and further enhancement of stem cell therapies. This paper provides a comprehensive review of human stem cells and polysaccharide hydrogels most used in regenerative medicine. The recent and advanced stem cell microencapsulation techniques, which include extrusion, emulsion, lithography, microfluidics, superhydrophobic surfaces and bioprinting, are described. This review also discusses current progress in clinical translation of stem-cell encapsulated polysaccharide hydrogels for cell delivery and disease modeling (drug testing and discovery) with focuses on musculoskeletal, nervous, cardiac and cancerous tissues.

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Section: Polysaccharide Hydrogelsmentioning

confidence: 99%

Section: Polysaccharide Hydrogelsmentioning

confidence: 99%

Polysaccharide-Based Hydrogels for Microencapsulation of Stem Cells in Regenerative Medicine

Lee

Khoo

et al. 2021

Front. Bioeng. Biotechnol.

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“…Given its biocompatibility, chitosan is widely used in pharmacological formulations and applications in tissue engineering, particularly for bone tissue. CS [158][159][160][161], HA [160,162,163], HEP [164], HS [161] and DS [158][159][160] have been combined with chitosan and proposed for skin wound healing [160], cartilage [158,159], bone [162] and vascular [164] applications.…”

Section: Chitosanmentioning

confidence: 99%

Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks

et al. 2020

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Glycosaminoglycans (GAG) are long, linear polysaccharides that display a wide range of relevant biological roles. Particularly, in the extracellular matrix (ECM) GAG specifically interact with other biological molecules, such as growth factors, protecting them from proteolysis or inhibiting factors. Additionally, ECM GAG are partially responsible for the mechanical stability of tissues due to their capacity to retain high amounts of water, enabling hydration of the ECM and rendering it resistant to compressive forces. In this review, the use of GAG for developing hydrogel networks with improved biological activity and/or mechanical properties is discussed. Greater focus is given to strategies involving the production of hydrogels that are composed of GAG alone or in combination with other materials. Additionally, approaches used to introduce GAG-inspired features in biomaterials of different sources will also be presented.

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“…Hydrogel systems have been broadly used in the development of delivery systems for drugs [17,18] and protein [19]. Because of their hydrophilic structure [10], natural polysaccharides in hydrogel form such as chitosan [6,20,21], alginate [10,22], starch [23], hyaluronic acid, and pectin [24] have been widely used in the fabrication of dressings for burned, exuding, and bleeding wounds. Pectin, one of the most abundant polysaccharides, is a promising biomaterial for tissue engineering and drug delivery systems due to its good biocompatibility, biodegradability, and antimicrobial activity [25].…”

Section: Introductionmentioning

confidence: 99%

Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release

et al. 2022

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Hypoalbuminemia can lead to poor and delayed wound healing, while it is also associated with acute myocardial infarction, heart failure, malignancies, and COVID-19. In elective surgery, patients with low albumin have high risks of postoperative wound complications. Here, we propose a novel cost-effective wound dressing material based on low-methoxy pectin and NaA-zeolite particles with controlled albumin release properties. We focused on both albumin adsorption and release phenomena for wounds with excess exudate. Firstly, we investigated albumin dynamics and calculated electrostatic surfaces at experimental pH values in water by using molecular dynamics methods. Then, we studied in detail pectin–zeolite hydrogels with both adsorption and diffusion into membrane methods using different pH values and albumin concentrations. To understand if uploaded albumin molecules preserved their secondary conformation in different formulations, we monitored the effect of pH and albumin concentration on the conformational changes in albumin after it was released from the hydrogels by using CD-UV spectroscopy analyses. Our results indicate that at pH 6.4, BSA-containing films preserved the protein’s folded structure while the protein was being released to the external buffer solutions. In vitro wound healing assay indicated that albumin-loaded hydrogels showed no toxic effects on the fibroblast cells.

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Design and evaluation of mesenchymal stem cells seeded chitosan/glycosaminoglycans quaternary hydrogel scaffolds for wound healing applications

Cited by 23 publications

References 44 publications

Polysaccharide-Based Hydrogels for Microencapsulation of Stem Cells in Regenerative Medicine

Polysaccharide-Based Hydrogels for Microencapsulation of Stem Cells in Regenerative Medicine

Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks

Pectin–Zeolite-Based Wound Dressings with Controlled Albumin Release

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