Design and evaluation of Konjac glucomannan-based bioactive interpenetrating network (IPN) scaffolds for engineering vascularized bone tissues

Kanniyappan, Hemalatha; Ponrasu, Thangavel; Chakraborty, Sudip; Arige, Vikas; Muthuvijayan, Vignesh

doi:10.1016/j.ijbiomac.2019.12.012

Cited by 32 publications

(17 citation statements)

References 56 publications

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“…The IPN scaffolds were found to have angiogenic activity, with details on the thickness and quantity of blood vessels seen, although histology is often recommended to reaffirm findings. 115 Biocompatibility of hydrophilic, porous, gelatin-polyvinylpyrrolidone (PVP) scaffolds was tested using the CAM assay by Mishra et al 45 Angiogenesis was not investigated or quantified in this study, with photographs taken at the time of material implantation and 3 days later, with no apparent indication of survival or normally developed chick numbers. It is reported that there was no effect on blood vessels compared with filter paper applied to each egg concurrently; therefore the material was deemed biocompatible due to lack of an obvious inflammatory response.…”

Section: Effect Of the Porosity And The Characteristics Of Angiogenicmentioning

confidence: 99%

Section: Application Of the Cam To Test Modified Polymer Materialsmentioning

confidence: 99%

“… 137 Other enhancements to aid vessel numeration include injecting an agent under the CAM from a distance to the scaffold, such as hand cream, providing a white background for contrast. 4 Software such as Photoshop, 4 Image J, 4 , 69 , 72 , 87 Angiotool 4 , 69 or AngioQuant (v1.33) 115 can be used to delineate the area of analysis, highlight or alter the image to demarcate the blood vessels more clearly, or perform automatic counting. Computer simulations have been investigated to automate the Chalkley counting process, making analysis more rapid and, critically, were observed to be as reliable as manual counting.…”

Section: Experimental Approaches To Quantify Angiogenesis and Assess mentioning

confidence: 99%

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Evolving applications of the egg: chorioallantoic membrane assay andex vivoorganotypic culture of materials for bone tissue engineering

2020

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The chick chorioallantoic membrane model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the chorioallantoic membrane assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the chorioallantoic membrane assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches, including timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo chorioallantoic membrane assay and the ex vivo organotypic culture to advance the field of bone tissue engineering, and highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field.

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Section: Effect Of the Porosity And The Characteristics Of Angiogenicmentioning

confidence: 99%

Section: Application Of the Cam To Test Modified Polymer Materialsmentioning

confidence: 99%

Section: Experimental Approaches To Quantify Angiogenesis and Assess mentioning

confidence: 99%

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Evolving applications of the egg: chorioallantoic membrane assay andex vivoorganotypic culture of materials for bone tissue engineering

2020

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“…KGM is suitable for the preparation of IPN due to its good biodegradability and gelling property. KGM is combined with synthetic polymers polyvinyl alcohol (PVA) and polycaprolactone (PCL) to produce bioactive blends that can be used as biological scaffolds for bone healing 35 …”

Section: Strategies For Strengthening the Functionality Of Kgmmentioning

confidence: 99%

Konjac glucomannan: A review of structure, physicochemical properties, and wound dressing applications

Zhou

Zheng

Xie

et al. 2021

J of Applied Polymer Sci

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Natural bio‐based materials used in wound dressings have been continuously developed in recent years, and have certain advantages in water retention, biocompatibility, and therapeutic properties. Konjac glucomannan (KGM) is a kind of plant polysaccharide with good cell compatibility and gel properties. Various gels obtained by modification of KGM through physical and chemical methods have great application potential in wound excipients. In the past few years, various KGM‐based wound barrier materials such as hydrogels, xerogel, microspheres, films, and fibers have been continuously researched and developed. The combination of KGM and other polymers can improve the problem of poor mechanical properties and give it new functional properties, which is beneficial to the moisturizing of the wound surface and the release of drugs to promote wound healing. This article reviews the physicochemical properties of KGM and the latest developments in KGM‐based composite gels, reveals the relationship between molecules in KGM‐based composite gels, and looks forward to the research and development prospects of KGM‐based composites in wound dressings.

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“…One of the strategies commonly used in the preparation of self-healing gels is the formation of interpenetrating polymer networks (IPNs). These are a type of hydrogel that comprise two or more networks that are at least partially interlaced at a polymer scale but are not covalently bound to each other [58,59]. More specifically, semi-IPNs (IPNs in which there is only one component of the assembly crosslinked, leaving the other in a linear form) can improve the self-healing capacity of the hydrogel by chains that flow through the interface at the damaged area [1,60,61].…”

Section: Mechanism Of Self-healing: Experimental and Theoretical Studiesmentioning

confidence: 99%

Autonomous self-healing hydrogel with anti-drying properties and applications in soft robotics

Naranjo

Martín

Lopez-Diaz

et al. 2020

Applied Materials Today

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Mimicking nature's self-healing ability has always been desired in science, especially when devices accumulate damage over time with performance, including the loss of function due to deterioration. SHAP (Self-Healing AETA([2-(acryloyloxy)ethyl]trimethylammonium chloride)based Polymer), a hydrogel with autonomous self-healing ability that can be applied for the development of a pneumatic artificial muscle, is presented here. Unlike other self-healing hydrogels, SHAP does not require any external stimulus to self-heal and it presents outstanding anti-drying properties. Few-layer graphene is also incorporated into the polymer network of the hydrogel in order to study the possible influence that the nanomaterial has on the properties of the scaffolds. The mechanical behavior and the self-healing abilities of the resulting hydrogels are analyzed. Moreover, the mechanism of self-healing is discussed in terms of experimental results and theoretical calculations. The data suggest a mechanism based on strong hydrogen-bonding interactions between the water molecules that remain inside SHAP, which keeps the material wet and soft under ambient conditions. Finally, the development of a SHAP-based artificial muscle is presented. The results show good performance of the healed artificial muscles after damage, even with healing periods as short as 10 minutes.

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Design and evaluation of Konjac glucomannan-based bioactive interpenetrating network (IPN) scaffolds for engineering vascularized bone tissues

Cited by 32 publications

References 56 publications

Evolving applications of the egg: chorioallantoic membrane assay andex vivoorganotypic culture of materials for bone tissue engineering

Evolving applications of the egg: chorioallantoic membrane assay andex vivoorganotypic culture of materials for bone tissue engineering

Konjac glucomannan: A review of structure, physicochemical properties, and wound dressing applications

Autonomous self-healing hydrogel with anti-drying properties and applications in soft robotics

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