Design and Evaluation of an Osteogenesis-on-a-Chip Microfluidic Device Incorporating 3D Cell Culture

Bahmaee, Hossein; Owen, Robert; Boyle, Liam; Perrault, Cécile M.; García-Granada, A. A.; Reilly, Gwendolen C.; Claeyssens, Frederik

doi:10.3389/fbioe.2020.557111

Cited by 52 publications

(59 citation statements)

References 71 publications

(90 reference statements)

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“…Claeyssens’ group prepared such a scaffold based on EHA, IBOA and TMPTA. This polyHIPE can be used as an osteogenesis-on-a-chip device for long-term cell culture, which can potentially replace animal testings, as it can mimic the 3-D environment present in tissues and organs [ 70 ].…”

Section: Combined and Advanced Applicationsmentioning

confidence: 99%

Porous Polymers from High Internal Phase Emulsions as Scaffolds for Biological Applications

2021

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High internal phase emulsions (HIPEs), with densely packed droplets of internal phase and monomers dispersed in the continuous phase, are now an established medium for porous polymer preparation (polyHIPEs). The ability to influence the pore size and interconnectivity, together with the process scalability and a wide spectrum of possible chemistries are important advantages of polyHIPEs. In this review, the focus on the biomedical applications of polyHIPEs is emphasised, in particular the applications of polyHIPEs as scaffolds/supports for biological cell growth, proliferation and tissue (re)generation. An overview of the polyHIPE preparation methodology is given and possibilities of morphology tuning are outlined. In the continuation, polyHIPEs with different chemistries and their interaction with biological systems are described. A further focus is given to combined techniques and advanced applications.

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Section: Combined and Advanced Applicationsmentioning

confidence: 99%

Porous Polymers from High Internal Phase Emulsions as Scaffolds for Biological Applications

2021

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“…Single-organ MPS have been constructed to serve as in vitro models for several different organs, such as the gastrointestinal (GI) tract ( Kim et al, 2012 ; Lee et al, 2018 ), heart ( Abulaiti et al, 2020 ), kidney ( Wilmer et al, 2016 ), bladder ( Sharma et al, 2021 ), liver ( Deng et al, 2020 ), brain ( Ndyabawe et al, 2021 ), lung ( Huh et al, 2010 ), skin ( Wufuer et al, 2016 ), skeletal muscle ( Grosberg et al, 2012 ), bone ( Bahmaee et al, 2020 ), vasculature ( Wang et al, 2018 ), immune-system ( Mitra et al, 2013 ), adipose tissue ( Liu et al, 2019 ), and reproductive tract ( Park et al, 2020 ). Here, we briefly discuss liver MPS, due to its significant role in drug metabolism, and a combination of single organ systems to establish more complex multi-organ MPS.…”

Section: Examples Of Mpsmentioning

confidence: 99%

Critical Considerations for the Design of Multi-Organ Microphysiological Systems (MPS)

Malik

Yang

Fathi

et al. 2021

Front. Cell Dev. Biol.

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Identification and approval of new drugs for use in patients requires extensive preclinical studies and clinical trials. Preclinical studies rely on in vitro experiments and animal models of human diseases. The transferability of drug toxicity and efficacy estimates to humans from animal models is being called into question. Subsequent clinical studies often reveal lower than expected efficacy and higher drug toxicity in humans than that seen in animal models. Microphysiological systems (MPS), sometimes called organ or human-on-chip models, present a potential alternative to animal-based models used for drug toxicity screening. This review discusses multi-organ MPS that can be used to model diseases and test the efficacy and safety of drug candidates. The translation of an in vivo environment to an in vitro system requires physiologically relevant organ scaling, vascular dimensions, and appropriate flow rates. Even small changes in those parameters can alter the outcome of experiments conducted with MPS. With many MPS devices being developed, we have outlined some established standards for designing MPS devices and described techniques to validate the devices. A physiologically realistic mimic of the human body can help determine the dose response and toxicity effects of a new drug candidate with higher predictive power.

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“…For example, microfluidic chip platforms have shown advantages over other 3D cell culture approaches due to the improved control of physiologic flow velocities, shear stresses, or oxygen gradients. Of note, on-chip systems such as osteogenesis-on-a-chip, 37 bone perivascular-niche-on-a-chip, 6 and bone-marrow-on-a-chip 57 have been reported. For example, a photocurable scaffold embedded within a silicone-based polymer seeded with MSCs was placed into a microfluidic chip for up to three weeks under physiologic flow conditions.…”

Section: Bioengineered Bone Microenvironment-based Strategies: From Simplest To Highly Complex Approachesmentioning

confidence: 99%

New insights into the biomimetic design and biomedical applications of bioengineered bone microenvironments

2021

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The bone microenvironment is characterized by an intricate interplay between cellular and noncellular components, which controls bone remodeling and repair. Its highly hierarchical architecture and dynamic composition provide a unique microenvironment as source of inspiration for the design of a wide variety of bone tissue engineering strategies. To overcome current limitations associated with the gold standard for the treatment of bone fractures and defects, bioengineered bone microenvironments have the potential to orchestrate the process of bone regeneration in a self-regulated manner. However, successful approaches require a strategic combination of osteogenic, vasculogenic, and immunomodulatory factors through a synergic coordination between bone cells, bone-forming factors, and biomaterials. Herein, we provide an overview of (i) current three-dimensional strategies that mimic the bone microenvironment and (ii) potential applications of bioengineered microenvironments. These strategies range from simple to highly complex, aiming to recreate the architecture and spatial organization of cell–cell, cell-matrix, and cell-soluble factor interactions resembling the in vivo microenvironment. While several bone microenvironment-mimicking strategies with biophysical and biochemical cues have been proposed, approaches that exploit the ability of the cells to self-organize into microenvironments with a high regenerative capacity should become a top priority in the design of strategies toward bone regeneration. These miniaturized bone platforms may recapitulate key characteristics of the bone regenerative process and hold great promise to provide new treatment concepts for the next generation of bone implants.

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Design and Evaluation of an Osteogenesis-on-a-Chip Microfluidic Device Incorporating 3D Cell Culture

Cited by 52 publications

References 71 publications

Porous Polymers from High Internal Phase Emulsions as Scaffolds for Biological Applications

Porous Polymers from High Internal Phase Emulsions as Scaffolds for Biological Applications

Critical Considerations for the Design of Multi-Organ Microphysiological Systems (MPS)

New insights into the biomimetic design and biomedical applications of bioengineered bone microenvironments

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