Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy

Landau, Sara; Liang, Chunjun; Kester, Robert T.; Tkaczyk, Tomasz S.; Descour, Michael R.

doi:10.1364/oe.18.004758

Cited by 17 publications

(17 citation statements)

References 14 publications

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“…Landau et al 16 reported that maintaining telecentricity in image space for such narrow objectives would compromise transverse magnification and performance at the edge of the FOV. Varying the principle ray angle from 0 deg, though it leads to some vignetting, helps to maintain higher image quality than if the design was doubly telecentric.…”

Section: Specifications For the Achromatic Ultra-slim Objectivementioning

confidence: 99%

Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective

et al. 2013

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Abstract. In order to diagnose cancer, a sample must be removed, prepared, and examined under a microscope, which is expensive, invasive, and time consuming. Fiber optic fluorescence endomicroscopy, where an image guide is used to obtain high-resolution images of tissue in vivo, has shown promise as an alternative to conventional biopsies. However, the resolution of standard endomicroscopy is limited by the fiber bundle sampling frequency and out-of-focus light. A system is presented which incorporates a plastic, achromatic objective to increase the sampling and which provides optical sectioning via structured illumination to reject background light. An image is relayed from the sample by a fiber bundle with the custom 2.1-mm outer diameter objective lens integrated to the distal tip. The objective is corrected for the excitation and the emission wavelengths of proflavine (452 and 515 nm). It magnifies the object onto the fiber bundle to improve the system's lateral resolution by increasing the sampling. The plastic lenses were fabricated via single-point diamond turning and assembled using a zero alignment technique. Ex vivo images of normal and neoplastic murine mammary tissues stained with proflavine are captured. The system achieves higher contrast and resolves smaller features than standard fluorescence endomicroscopy.

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Section: Specifications For the Achromatic Ultra-slim Objectivementioning

confidence: 99%

Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective

et al. 2013

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“…Compared to a previous ultra-slim objective design [25], this objective has been modified for use with multiphoton imaging and to improve tolerancing of the design based on assembly procedures. The design has been incorporated into a 1.27 mm O.D.…”

Section: Overview Of the Ultra-slim Endomicroscope Objectivementioning

confidence: 99%

Ultra-slim plastic endomicroscope objective for non-linear microscopy

Kyrish¹,

Utzinger²,

Descour³

et al. 2011

Opt. Express

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Non-linear microscopy has the potential to provide clinically useful information on the structure of biological tissue in vivo via an endomicroscope. The ability to use plastic as the optical material in a multiphoton objective was evaluated based on several criteria including autofluorescence, injection molding induced birefringence, and pulse broadening due to group velocity dispersion. An all-plastic, refractive ultra-slim endoscope objective was built with design specifications of NA = 0.4, FOV = 250 μm, 1.27 mm outer diameter, and 0.8 mm clear aperture. Initial images of second-harmonic generation signal (illumination at 780 nm) in collagen fibers and two-photon excited fluorescence (illumination at 920 nm) of Convallaria rhizome are reported.

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“…Multi-photon fluorescence microscopy allows for inherent optical sectioning but requires expensive pico-or femto-second pulsed lasers and a bulky scanning mechanism incorporated in the head of the probe [1][2][3][4]39]. Approaches have been established, which externalize the scanning unit by utilizing a multicore fiber [40][41][42] and therewith tremendously miniaturized the size of the rigid tip. Yet, a finite bending radius of the flexible part comes along with a complex and costly coupling setup at the proximal side of the fiber probe and hence impede the transfer into the daily healthcare practice and research community.…”

Section: Introductionmentioning

confidence: 99%

Chip-on-the-tip compact flexible endoscopic epifluorescence video-microscope for in-vivo imaging in medicine and biomedical research

Matz

Messerschmidt

Göbel

et al. 2017

Biomed. Opt. Express

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Abstract:We demonstrate a 60 mg light video-endomicroscope with a cylindrical shape of the rigid tip of only 1.6 mm diameter and 6.7 mm length. A novel implementation method of the illumination unit in the endomicroscope is presented. It allows for the illumination of the biological sample with fiber-coupled LED light at 455 nm and the imaging of the red-shifted fluorescence light above 500 nm in epi-direction. A large numerical aperture of 0.7 leads to a sub-cellular resolution and yields to high-contrast images within a field of view of 160 µm. A miniaturized chip-on-the-tip CMOS image sensor with more than 150,000 pixels captures the multicolor images at 30 fps. Considering size, plug-and-play capability, optical performance, flexibility and weight, we hence present a probe which sets a new benchmark in the field of epifluorescence endomicroscopes. Several ex-vivo and in-vivo experiments in rodents and humans suggest future application in biomedical fields, especially in the neuroscience community, as well as in medical applications targeting optical biopsies or the detection of cellular anomalies.

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Design and evaluation of an ultra-slim objective for in-vivo deep optical biopsy

Cited by 17 publications

References 14 publications

Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective

Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective

Ultra-slim plastic endomicroscope objective for non-linear microscopy

Chip-on-the-tip compact flexible endoscopic epifluorescence video-microscope for in-vivo imaging in medicine and biomedical research

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