Design and economics of industrial production of fructooligosaccharides

Abstract

A process for industrial production of fructooligosaccharides (FOS’s) based on the conversion of sucrose by immobilized fructosyltransferase (FTase) from the cells of Aureobasidium pullulans CCY 27-1-94 was developed. Particular process operations and conditions were designed employing results of laboratory and semi-pilot scale experiments. The process flowsheet comprised three sections: FTase production, which included fermentation, isolation and purification of the enzyme, FTase immobilization and FOS’s production where a product with a high content of FOS’s was prepared by the removal of glucose, fructose and unreacted sucrose from the reaction mixture using simulated moving-bed chromatography. Two alternative process flowsheets were proposed for the annual production of 10 000 t of FOS’s: one for a powdery product and the second one for syrup. The economic analysis provided the costs for the production of immobilized FTase and FOS’s using two different price estimates for sucrose.

An efficient continuous flow process for the synthesis of a non-conventional mixture of fructooligosaccharides

“…Industrial scale production of FOS is commonly performed by either soluble enzymes in batch reactions (Hidaka, Eida, Adachi, & Saitoh, 1987) or by immobilized enzymes using continuous fixed-bed reactors (Park, Lim, Kim, Park, & Kim, 2005;Yun, Kang, & Song, 1995). Immobilized FTase from Aureobasidium pullulans and the immobilized whole cells have been used in packed bed reactors for the continuous production of FOS at a plant scale (Jung, Bang, Oh, & Park, 2011;Vaň ková, Onderková, Antošová, & Polakovič, 2008).…”

Microbial production of fructosyltransferases for synthesis of pre-biotics

“…Concerning studies about scale-up and economic feasibility, a batch production process of purified FTase from Aureobasidium pullulans was designed (Vaňková et al 2005). The plant was designed for the annual production capacity of 80 kg enzyme powder with a specific FTase activity of 1,143,000 U/g and contained 28.4% FTase.…”

Comparison Between Systems for Synthesis of Fructooligosaccharides from Sucrose Using Free Inulinase from Kluyveromyces marxianus NRRL Y-7571

“…The overall stoichiometry of the inulinase action on sucrose can be characterized by two parallel reaction paths (Vanková et al 2008). A set of disproportionate reactions provides FOS and glucose (as a by-product) from the fructosyltransferase activity whereas the enzyme's hydrolytic activity results in the formation of the glucose and the fructose as by-products (Aboudzadeh et al 2006).…”