Design and development of portable fluorescence reader using silicon photo multiplier (SiPM) sensor

Makkar, Roshan; Aliya, Sana Syeda; Borse, Vivek; Srivastava, Rohit

doi:10.1117/12.2305846

“…The SiPM was used to measure the output signal from a fluorescent lateral flow assay (FLFA). The FLFA contains cadmium telluride quantum dots capped with mercaptopropionic acid (MPA-CdTe QDs) to detect the QD concentration in a range of 60–420 μg/mL . However, the applicability of the device was not explored for pH or urea sensing.…”

Section: Introductionmentioning

confidence: 99%

“…The FLFA contains cadmium telluride quantum dots capped with mercaptopropionic acid (MPA-CdTe QDs) to detect the QD concentration in a range of 60−420 μg/mL. 21 However, the applicability of the device was not explored for pH or urea sensing. In another similar work, Vaghela et al developed an LED-based urea biosensor to detect urea levels in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Translation of Carbon Dot Biosensors into an Embedded Optical Setup for Spoilage and Adulteration Detection

Choudhary

¹

,

Joshi

²

,

Joshi

³

2021

ACS Food Sci. Technol.

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This research demonstrates a carbon quantum dot (CQD) based fluorescence technique for milk quality assessment and detection of urea adulteration using a commercialized optical fiber spectrometer (OFS) and a developed color sensor device (CSD). The change in pH from the normal pH of milk (∼6.7) indicates spoilage. The CQDs were synthesized using phthalic acid (carbon source) and triethylenediamine (TED) (passivant). The CQDs were found to be sensitive in the pH range 3−10 with a quantum yield of about 18.9%. The accuracy of spoilage detection using CQDs was expressed in the form of mean percent recovery and found to be 99.2% (R 2 = 0.97) by the OFS and 99.59% (R 2 = 0.98) by the CSD. The mean percent recovery obtained for urea adulteration in milk was 103.0% (R 2 = 0.98) by the OFS and 97.9% (R 2 = 0.97) by the CSD. The results indicate that CQDs have excellent potential for use as biosensors for spoilage and adulteration detection.

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“…15,16 Low frequency or constant power applications have also begun to emerge for projects focused on providing affordable instrumentation. 11,17,18 We sought to apply a simple circuit for the SiPM detector for use with a detection apparatus and signal processing workflow designed for on-column epifluorescence detection. By doing so, we found the SiPM can obtain a molar LOD of 10 ■ EXPERIMENTAL SECTION Materials and Reagents.…”

mentioning

confidence: 99%

“…Each microcell produces a digital current signal when struck by a photon, with the entire array producing an analog signal at higher light densities . Several methods have emerged for deploying a SiPM in experiments for photon counting where a pulsed light source and a high bandwidth analog to digital converter (ADC) or oscilloscope are used. , Low frequency or constant power applications have also begun to emerge for projects focused on providing affordable instrumentation. ,, We sought to apply a simple circuit for the SiPM detector for use with a detection apparatus and signal processing workflow designed for on-column epifluorescence detection. By doing so, we found the SiPM can obtain a molar LOD of 10 –21 moles and span a linear dynamic range of 5–6 orders of magnitude, thus, justifying SiPM use in affordable chemical cytometry instrumentation and particularly CE-based instruments.…”

mentioning

confidence: 99%

Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

Petersen

¹

,

Gallion

²

,

Allbritton

³

2020

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Capillary electrophoresis (CE) is a highly efficient separation method capable of handling small sample volumes (~pL) and low (~yoctomole) detection limits, and as such is ideal for applications that require high sensitivity such as single-cell analysis. Low-cost CE instrumentation is quickly expanding but low-cost, open-source fluorescence detectors with ultra-sensitive detection limits are lacking. Silicon photomultipliers (SiPM) are inexpensive, low-footprint detectors with the potential to fill the role as a detector when cost, size, and customization are important. In this work we demonstrate the use of a SiPM in CE with zeptomolar detection limits and a dynamic range spanning five orders of magnitude, comparable to photomultiplier detectors. We characterize the performance of the SiPM as a highly sensitive detector by measuring enzyme activity in single cells. This simple, small footprint, and low-cost (<$130) light detection circuit will be beneficial for open-source, portable, and budget friendly instrumentation requiring high sensitivity. File list (2) download file view on ChemRxiv SiPM_CE_Detector.pdf (342.26 KiB) download file view on ChemRxiv Supplemental_Information.pdf (308.35 KiB)

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“…17,18 Low frequency or constant power applications have also begun to emerge for projects focused on providing affordable instrumentation. 13,19,20 We sought to apply a simple circuit for the SiPM detector for use with a detection apparatus and signal processing workflow designed for oncolumn epifluorescence detection. By doing so, we found the SiPM can obtain a molar LOD of 10 -21 moles and span a linear dynamic range of 5-6 orders of magnitude, thus justifying.…”

mentioning

confidence: 99%

Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

Petersen

¹

,

Gallion²,

Allbritton³

2020

Preprint

View full text Add to dashboard Cite

Capillary electrophoresis (CE) is a highly efficient separation method capable of handling small sample volumes (~pL) and low (~yoctomole) detection limits, and as such is ideal for applications that require high sensitivity such as single-cell analysis. Low-cost CE instrumentation is quickly expanding but low-cost, open-source fluorescence detectors with ultra-sensitive detection limits are lacking. Silicon photomultipliers (SiPM) are inexpensive, low-footprint detectors with the potential to fill the role as a detector when cost, size, and customization are important. In this work we demonstrate the use of a SiPM in CE with zeptomolar detection limits and a dynamic range spanning five orders of magnitude, comparable to photomultiplier detectors. We characterize the performance of the SiPM as a highly sensitive detector by measuring enzyme activity in single cells. This simple, small footprint, and low-cost (<$130) light detection circuit will be beneficial for open-source, portable, and budget friendly instrumentation requiring high sensitivity.<br>

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Design and development of portable fluorescence reader using silicon photo multiplier (SiPM) sensor

Cited by 8 publications

References 22 publications

Translation of Carbon Dot Biosensors into an Embedded Optical Setup for Spoilage and Adulteration Detection

Translation of Carbon Dot Biosensors into an Embedded Optical Setup for Spoilage and Adulteration Detection

Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis

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