Design and development of plastic antibodies against SARS-CoV-2 RBD based on molecularly imprinted polymers that inhibit<i>in vitro</i>virus infection

Parisi, Ortensia Ilaria; Dattilo, Marco; Patitucci, Francesco; Malivindi, Rocco; Delbue, Serena; Ferrante, Pasquale; Parapini, Silvia; Galeazzi, Roberta; Cavarelli, Mariangela; Cilurzo, Francesco; Franzè, Silvia; Perrotta, Ida; Pezzi, Vincenzo; Selmin, Francesca; Ruffo, Mariarosa; Puoci, Francesco

doi:10.1039/d1nr03727g

Cited by 28 publications

(21 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, MIPs are potentially applicable for the treatment of infectious pathologies because they can recognize and block components of etiological agents, such as bacteria, viruses, fungi and protista, involved in infection mechanisms. For example, Parisi et al [ 183 ] used non-covalent imprinting to prepare a MIP able to recognize and bind the receptor-binding domain of the spike protein of SARS-CoV-2 (COVID-19 etiological agent), a surface glycoprotein involved in the recognition and the attachment to host cells [ 184 ]. The obtained imprinted nanoparticles were able to significantly inhibit virus replication in Vero cells culture.…”

Section: Molecularly Imprinted Polymers (Mips)mentioning

confidence: 99%

The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart

Parisi

Francomano

Dattilo

et al. 2022

JFB

Self Cite

View full text Add to dashboard Cite

Molecular recognition is a useful property shared by various molecules, such as antibodies, aptamers and molecularly imprinted polymers (MIPs). It allows these molecules to be potentially involved in many applications including biological and pharmaceutical research, diagnostics, theranostics, therapy and drug delivery. Antibodies, naturally produced by plasma cells, have been exploited for this purpose, but they present noticeable drawbacks, above all production cost and time. Therefore, several research studies for similar applications have been carried out about MIPs and the main studies are reported in this review. MIPs, indeed, are more versatile and cost-effective than conventional antibodies, but the lack of toxicity studies and their scarce use for practical applications, make it that further investigations on this kind of molecules need to be conducted.

show abstract

Section: Molecularly Imprinted Polymers (Mips)mentioning

confidence: 99%

The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart

Parisi

Francomano

Dattilo

et al. 2022

JFB

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nevertheless, the synthesis of molecularly imprinted polymers (MIPs) was boosted by Wulff and Mosbach later in the 1970s [ 22 , 23 ]. Since then, a broad spectrum of analytes including low-molecular-weight molecules, such as pharmaceuticals, sugars, toxins, narcotic drugs, pesticides, and biomacromolecules such as proteins, nucleic acids, bacteria, and viruses have been described [ 20 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ].…”

Section: Molecularly Imprinted Polymersmentioning

confidence: 99%

Molecularly Imprinted Polymer-Based Sensors for SARS-CoV-2: Where Are We Now?

Yarman

Kurbanoğlu

2022

Biomimetics

View full text Add to dashboard Cite

Since the first reported case of COVID-19 in 2019 in China and the official declaration from the World Health Organization in March 2021 as a pandemic, fast and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has played a major role worldwide. For this reason, various methods have been developed, comprising reverse transcriptase-polymerase chain reaction (RT-PCR), immunoassays, clustered regularly interspaced short palindromic repeats (CRISPR), reverse transcription loop-mediated isothermal amplification (RT-LAMP), and bio(mimetic)sensors. Among the developed methods, RT-PCR is so far the gold standard. Herein, we give an overview of the MIP-based sensors utilized since the beginning of the pandemic.

show abstract

“…MIPs have demonstrated selective recognition and the ability to selectively rebind a model mammalian virus [ 34 ]. Subsequent to this research, Parisi et al [ 35 ] demonstrated that nanoparticle-based MIPs were able to inhibit binding of SARS-CoV-2 to its cellular receptor, ACE2. Such examples of virus neutralisation and inhibition open opportunities for MIPs to be used as antiviral agents.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of electropolymerized molecularly imprinted polymers (E-MIPs) on disposable electrodes for detection of SARS-CoV-2 in saliva

EL-Sharif

Dennison

Tully

et al. 2022

Analytica Chimica Acta

View full text Add to dashboard Cite

Design and development of plastic antibodies against SARS-CoV-2 RBD based on molecularly imprinted polymers that inhibitin vitrovirus infection

Abstract: The present research study reports the development of plastic antibodies based on Molecularly Imprinted Polymers (MIPs) able to selectively bind a portion of the novel coronavirus SARS-CoV-2 spike protein. Indeed,...

Cited by 28 publications

References 42 publications

The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart

The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart

Molecularly Imprinted Polymer-Based Sensors for SARS-CoV-2: Where Are We Now?

Evaluation of electropolymerized molecularly imprinted polymers (E-MIPs) on disposable electrodes for detection of SARS-CoV-2 in saliva

Contact Info

Product

Resources

About