Design and Development of Photonic Biosensors for Swine Viral Diseases Detection

Griol, Amadeu; Peransi, Sergio; Rodrigo, Manuel A.; Hurtado, Juan; Bellières, Laurent; Ivanova, T.Y.; Zurita, David; Sánchez, Carles; Recuero, S.; Hernández, Adolfo; Simón, Santiago; Balka, Gyula; Bossis, Ioannis; Capo, Alessandro; Camarca, Alessandra; D’Auria, Sabato; Varriale, Antonio; Giusti, Alessandro

doi:10.3390/s19183985

Cited by 11 publications

(14 citation statements)

References 25 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wave-length-interrogation is adopted to monitor the shifts that the notch resonance undergoes when the environment near the surface of the sensor changes. The biosensor Photonic Integrated Circuit (PIC) consists of eight ring resonators (RRs) [ 13 ], as shown in Figure 1 a. The whole PIC is covered by a SiO 2 layer (1000 nm width) to optimize the performance of the Grating Couplers (GC), and, in addition, as a protective layer.…”

Section: Methodsmentioning

confidence: 99%

“…A window is opened around the RRs to allow access for the functionalization of their surfaces and their exposure to the sample, as shown in Figure 1 b. The fabrication details of the developed PICs are further described in an authors’ previous work [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…RRs were fabricated in silicon nitride and functionalized with antibodies to capture the targeted viral antigens. The objectives of this study were (i) to assess the performance of commercial antibodies used as Molecular Recognition Elements (MREs), (ii) to biofunctionalize RRs with the selected MREs, and (iii) to detect the targeted viruses with the novel biosensors using reference samples (cell culture supernatants) as proof of concept [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases

Navarro-Arenas

Sánchez²,

Peransi-Llopis³

et al. 2022

Sensors

Self Cite

View full text Add to dashboard Cite

In this paper we present the development of photonic integrated circuit (PIC) biosensors for the label-free detection of six emerging and endemic swine viruses, namely: African Swine Fever Virus (ASFV), Classical Swine Fever Virus (CSFV), Porcine Reproductive and Respiratory Syndrome Virus (PPRSV), Porcine Parvovirus (PPV), Porcine Circovirus 2 (PCV2), and Swine Influenza Virus A (SIV). The optical biosensors are based on evanescent wave technology and, in particular, on Resonant Rings (RRs) fabricated in silicon nitride. The novel biosensors were packaged in an integrated sensing cartridge that included a microfluidic channel for buffer/sample delivery and an optical fiber array for the optical operation of the PICs. Antibodies were used as molecular recognition elements (MREs) and were selected based on western blotting and ELISA experiments to ensure the high sensitivity and specificity of the novel sensors. MREs were immobilized on RR surfaces to capture viral antigens. Antibody–antigen interactions were transduced via the RRs to a measurable resonant shift. Cell culture supernatants for all of the targeted viruses were used to validate the biosensors. Resonant shift responses were dose-dependent. The results were obtained within the framework of the SWINOSTICS project, contributing to cover the need of the novel diagnostic tools to tackle swine viral diseases.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases

Navarro-Arenas

Sánchez²,

Peransi-Llopis³

et al. 2022

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…PICs were provided by Universitat Politècnica de València Nanophotonics and Technology Center and Lumensia Sensors S.L. and were fabricated and functionalized as previously described by Griol et al [ 30 ]. The dimensions of the microfluidic board of PICs were 7 × 3 cm, and the board was fabricated in cyclic olefin polymer (COP).…”

Section: Methodsmentioning

confidence: 99%

Integration of Microfluidics, Photonic Integrated Circuits and Data Acquisition and Analysis Methods in a Single Platform for the Detection of Swine Viral Diseases

Manessis

Mourouzis

Griol

et al. 2021

Animals

Self Cite

View full text Add to dashboard Cite

Viral diseases challenge the health and welfare of pigs and undermine the sustainability of swine farms. Their efficient control requires early and reliable diagnosis, highlighting the importance of Point of Care (POC) diagnostics in veterinary practice. The objective of this study was to validate a novel POC system that utilizes Photonic Integrated Circuits (PICs) and microfluidics to detect swine viral pathogens using oral fluids and Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV-2) as proofs of concept. The sensitivity and specificity of the device were calculated for both viruses, and Receiver Operating Characteristic (ROC) curves were drawn. PPV had an Area Under Curve (AUC) value of 0.820 (95% CI: 0.760 to 0.880, p < 0.0001), and its optimal efficiency threshold of detection shifts was equal to 4.5 pm (68.6% sensitivity, 77.1% specificity and Limit of Detection (LOD) value 106 viral copies/mL). PCV-2 had an AUC value of 0.742 (95% CI: 0.670 to 0.815, p < 0.0001) and an optimal efficiency threshold of shifts equal to 6.5 pm (69.5% sensitivity, 70.3% specificity and LOD 3.3 × 105 copies/mL). In this work, it was proven that PICs can be exploited for the detection of swine viral diseases. The novel device can be directly deployed on farms as a POC diagnostics tool.

show abstract

“…The author also performed the simulation studies, where the simulation results confirm the SPP's tighter spatial confinement and higher local field strength, which aids in detecting slight changes in the refractive index on the sensor surface. A field diagnostic system based on a combination of advanced bio-sensing and photonics technologies for the detection of swine virus was proposed by Griol et al [206]. The plan was focused on the use of CMOS (Complementary Metal Oxide Semiconductor) compatible microring resonators fabricated in silicon nitride.…”

Section: Photonic Sensorsmentioning

confidence: 99%

Optical Biosensors for Diagnostics of Infectious Viral Disease: A Recent Update

et al. 2021

View full text Add to dashboard Cite

The design and development of biosensors, analytical devices used to detect various analytes in different matrices, has emerged. Biosensors indicate a biorecognition element with a physicochemical analyzer or detector, i.e., a transducer. In the present scenario, various types of biosensors have been deployed in healthcare and clinical research, for instance, biosensors for blood glucose monitoring. Pathogenic microbes are contributing mediators of numerous infectious diseases that are becoming extremely serious worldwide. The recent outbreak of COVID-19 is one of the most recent examples of such communal and deadly diseases. In efforts to work towards the efficacious treatment of pathogenic viral contagions, a fast and precise detection method is of the utmost importance in biomedical and healthcare sectors for early diagnostics and timely countermeasures. Among various available sensor systems, optical biosensors offer easy-to-use, fast, portable, handy, multiplexed, direct, real-time, and inexpensive diagnosis with the added advantages of specificity and sensitivity. Many progressive concepts and extremely multidisciplinary approaches, including microelectronics, microelectromechanical systems (MEMSs), nanotechnologies, molecular biology, and biotechnology with chemistry, are used to operate optical biosensors. A portable and handheld optical biosensing device would provide fast and reliable results for the identification and quantitation of pathogenic virus particles in each sample. In the modern day, the integration of intelligent nanomaterials in the developed devices provides much more sensitive and highly advanced sensors that may produce the results in no time and eventually help clinicians and doctors enormously. This review accentuates the existing challenges engaged in converting laboratory research to real-world device applications and optical diagnostics methods for virus infections. The review’s background and progress are expected to be insightful to the researchers in the sensor field and facilitate the design and fabrication of optical sensors for life-threatening viruses with broader applicability to any desired pathogens.

show abstract

Design and Development of Photonic Biosensors for Swine Viral Diseases Detection

Cited by 11 publications

References 25 publications

Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases

Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases

Integration of Microfluidics, Photonic Integrated Circuits and Data Acquisition and Analysis Methods in a Single Platform for the Detection of Swine Viral Diseases

Optical Biosensors for Diagnostics of Infectious Viral Disease: A Recent Update

Contact Info

Product

Resources

About