Design and development of PCR-free highly sensitive electrochemical assay for detection of telomerase activity using Nano-based (liposomal) signal amplification platform

Alizadeh-Ghodsi, Mohammadreza; Zavari-Nematabad, Ali; Hamishehkar, Hamed; Akbarzadeh, Abolfazl; Mahmoudi-Badiki, Tohid; Zarghami, Faraz; Pourhassan‐Moghaddam, Mohammad; Alipour, Esmaeel; Zarghami, Nosratollah

doi:10.1016/j.bios.2016.01.090

Cited by 45 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, liposomes have been used to increase water solubility of 17-AAG. Liposomes are nanocarriers composed of lipid bilayers surrounding aqueous compartments (Alizadeh-Ghodsi et al, 2016;Zavari-Nematabad et al, 2017); they are able to carry both hydrophilic and hydrophobic molecules (Heneweer, Gendy, & Peñate-Medina, 2012). The unique advantages of liposomes including biodegradability, biocompatibility, protection of drug from degradation, and targeting of the drug to the site of action.…”

Section: Encapsulated 17-aag In Liposomementioning

confidence: 99%

Spotlight on 17‐AAG as an Hsp90 inhibitor for molecular targeted cancer treatment

et al. 2019

Self Cite

View full text Add to dashboard Cite

Hsp90 is a ubiquitous chaperone with important roles in the organization and maturation of client proteins that are involved in the progression and survival of cancer cells. Multiple oncogenic pathways can be affected by inhibition of Hsp90 function through degradation of its client proteins. That makes Hsp90 a therapeutic target for cancer treatment. 17‐allylamino‐17‐demethoxy‐geldanamycin (17‐AAG) is a potent Hsp90 inhibitor that binds to Hsp90 and inhibits its chaperoning function, which results in the degradation of Hsp90's client proteins. There have been several preclinical studies of 17‐AAG as a single agent or in combination with other anticancer agents for a wide range of human cancers. Data from various phases of clinical trials show that 17‐AAG can be given safely at biologically active dosages with mild toxicity. Even though 17‐AAG has suitable pharmacological potency, its low water solubility and high hepatotoxicity could significantly restrict its clinical use. Nanomaterials‐based drug delivery carriers may overcome these drawbacks. In this paper, we review preclinical and clinical research on 17‐AAG as a single agent and in combination with other anticancer agents. In addition, we highlight the potential of using nanocarriers and nanocombination therapy to improve therapeutic effects of 17‐AAG.

show abstract

Section: Encapsulated 17-aag In Liposomementioning

confidence: 99%

Spotlight on 17‐AAG as an Hsp90 inhibitor for molecular targeted cancer treatment

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Dopamine-loaded biotinylated liposome was used to construct a highly sensitive electrochemical assay for the detection of telomerase activity in A549 cells 31. In the study, carbon nanotubes-decorated GCE was used to enhance the sensitivity of the dopamine detection.…”

Section: Assays For the Detection Of Telomerase Activity Using Isothementioning

confidence: 99%

Advances in the detection of telomerase activity using isothermal amplification

Zhang¹,

Lou²,

Xia³

2017

Theranostics

View full text Add to dashboard Cite

Telomerase plays a significantly important role in keeping the telomere length of a chromosome. Telomerase overexpresses in nearly all tumor cells, suggesting that telomerase could be not only a promising biomarker but also a potential therapeutic target for cancers. Therefore, numerous efforts focusing on the detection of telomerase activity have been reported from polymerase chain reaction (PCR)-based telomeric repeat amplification protocol (TRAP) assays to PCR-free assays such as isothermal amplification in recent decade. In this review, we highlight the strategies for the detection of telomerase activity using isothermal amplification and discuss some of the challenges in designing future telomerase assays as well.

show abstract

“…According to evidence, the first example of peptide-functionalized GNPs is hydrolytically active against carboxylate esters. The active units are constituted by His-Phe-OH terminating thiols [41,42]. A highly sensitive and selective fluorescent assay for the detection of acetylcholine (ACh) was developed based on enzyme mimics of Au/Ag NPs [43].…”

Section: Esterase Mimicmentioning

confidence: 99%

Gold nanoparticles applications: from artificial enzyme till drug delivery

Golchin

Ghaderi

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

View full text Add to dashboard Cite

Today, nano-medicine promotes new therapeutics and diagnostics tools, including sensing of biomolecules as a biosensor, cancer chemotherapy and drug or gene delivery. Because of small size and biocompatibility of gold nanoparticles (GNPs), they become a good candidate for biological application. Also, thanks to their biological and chemical properties, they can mimic function of some enzymes including super oxide dismutase (SOD), esterase, etc. Also, biomaterials and bioengineering have grown so fast since the last decade for many therapeutic applications such as tissue regeneration. Among these cutting edge technology, nanomaterials find the way to becoming a very powerful tool for using in many fields of researchers including biosensing, gene therapy and chemotherapy. In this review, we focused on some biological applications of GNPs in biology and medicine.

show abstract

Design and development of PCR-free highly sensitive electrochemical assay for detection of telomerase activity using Nano-based (liposomal) signal amplification platform

Cited by 45 publications

References 26 publications

Spotlight on 17‐AAG as an Hsp90 inhibitor for molecular targeted cancer treatment

Spotlight on 17‐AAG as an Hsp90 inhibitor for molecular targeted cancer treatment

Advances in the detection of telomerase activity using isothermal amplification

Gold nanoparticles applications: from artificial enzyme till drug delivery

Contact Info

Product

Resources

About