Design and development of low cost Nano drop UV-Vis spectrometer

Abstract

The ultimate aim of the project is to develop a low-cost spectrometer that analyses the samples in the Nanoscale range and to minimise the usage of samples for diagnostic application. The reflection, absorption or transmission phenomenon alters the incident light during the interaction with the sample. A spectrometer measures this change over a range of incident wavelengths of electromagnetic radiation that has interacted with a sample. The light that acts as a source is passed through the sample. The prepared test sample is analysed in order to validate the developed system. Following the test samples, urine is analysed. 10 volunteers were involved as subjects. The light from the sample passes through a slit and then reflected by a collimating mirror. The wavelength of the light from the sample is analysed by Thermino software in the UV and visible range. The input to this software is provided by the webcam. A 1000 lines/mm diffraction grating is utilized to split the light into its constituent wavelength. This is a low-cost system than the available commercial spectrometers used in the laboratory.