Design and development of caffeic acid conjugated with Bombyx mori derived peptide biomaterials for anti-aging skin care applications

Abstract

This study examined the significant features of caffeic acid conjugated with peptide (APPPKK) to determine if it meets the requirements for a cosmetic anti-aging biomolecule.

Antioxidant and anti-aging potential of a peptide formulation (Gal2–Pep) conjugated with gallic acid

“…Some studies have shown that ascorbic acid is stabilized by binding it with peptides. 12,25 We believe that GA may be stabilized by binding it with a peptide in the same way.…”

“…DPPH assay were performed following a previously reported methodology with minor modification. 12,14–16 First, a 0.12 mg mL −1 DPPH solution was prepared in methanol, then 100 μL of DPPH solution and 100 μL of the synthesized peptides or GA were mixed at different concentrations (0.1 mM, 0.05 mM, 0.01 mM, and 1 μM) in 96-well microplates. In an orbital shaking incubator, the mixtures were reacted for 30 min at 37 °C and 100 rpm without light.…”

Metasequoia glyptostroboides potentiates anticancer effect against cervical cancer via intrinsic apoptosis pathway

“…The detection of ROS generation assay was according to previous studies with some modification 16 . To detect ROS at chemical level, a 500 µl sample of 1 mM 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA; 287810, MERCK MILLIPORE, German) was reacted with 10 mM NaOH (2 ml) for 30 min to achieve complete deacetylation in the darkroom.…”

Anti-Aging Potential of Bioactive Phytoconstituents Found in Edible Medicinal Plants: A Review

“…By synthesizing caffeic acid with a bioactive peptide (APPPKK), Lee et al [136] examined the effect of the conjugate on skin aging. The caffeic acid and peptide conjugate were found to be biocompatible as the cell viability essay revealed the 90% viability of fibroblast cells, even at a concentration of 100 µM.…”