Design and development of biomimetic electrospun sulphonated polyether ether ketone nanofibrous scaffold for bone tissue regeneration applications: <i>in vitro</i> and <i>in vivo</i> study

Ekambaram, Rajalakshmi; Dharmalingam, Sangeetha

doi:10.1080/09205063.2022.2025637

Cited by 2 publications

(2 citation statements)

References 75 publications

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“…3 Calcium phosphate, ceramics, Polyetheretherketone, and polymers are some common tissue engineering materialsthat have been used as bone replacement materials. 4,5 However, these replacement materials do not function as filler-like materials to induce bone regeneration in situ, and the techniques of these methods face problems of immune rejection, poor degradability, and poor bioactivity, making it difficult to achieve the expected efficacy. 6 Chitosan, polylactic acid, alginate and other bone-inducing materials have problems such as weak osteogenic activity, insufficient mechanical strength, and poor biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

VEGF-modified PLA/HA nanocomposite fibrous membrane for cranial defect repair in rats

Wang,

Li,

Zhao

et al. 2023

J Biomater Appl

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A major obstacle to bone tissue repair is the difficulty in establishing a rapid blood supply areas of bone defects. Vascular endothelial growth factor (VEGF)-infused tissue-engineered scaffolds offer a possible therapeutic option for these types of injuries. Their role is to accelerate angiogenesis and improve bone healing. In this study, we used electrostatic spinning and biofactor binding to construct polylactic acid (PLA)/hydroxyapatite (HA)-VEGF scaffold materials and clarify their pro-vascular role in bone defect areas for efficient bone defect repair. PLA/HA nanocomposite fibrous membranes were manufactured by selecting suitable electrostatic spinning parameters. Heparin and VEGF were bound sequentially, and then the VEGF binding and release curves of the fiber membranes were calculated. A rat cranial defect model was constructed, and PLA/HA fiber membranes bound with VEGF and unbound with VEGF were placed for treatment. Finally, we compared bone volume recovery and vascular recovery in different fibrous membrane sites. A VEGF concentration of 2.5 µg/mL achieved the maximum binding and uniform distribution of PLA/HA fibrous membranes. Extended-release experiments showed that VEGF release essentially peaked at 14 days. In vivo studies showed that PLA/HA fibrous membranes bound with VEGF significantly increased the number of vessels at the site of cranial defects, bone mineral density, bone mineral content, bone bulk density, trabecular separation, trabecular thickness, and the number of trabeculae at the site of defects in rats compared with PLA/HA fibrous membranes not bound with VEGF. VEGF-bound PLA/HA fibrous membranes demonstrate the slow release of VEGF. The VEGF binding process does not disrupt the morphology and structure of the fibrous membranes. The fibrous membranes could stimulate both osteogenesis and angiogenesis. Taken together, this research provides a new strategy for critical-sized bone defects repairing.

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Section: Introductionmentioning

confidence: 99%

VEGF-modified PLA/HA nanocomposite fibrous membrane for cranial defect repair in rats

Wang,

Li,

Zhao

et al. 2023

J Biomater Appl

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“…Considering bone tissue engineering, scaffolds are more suitable than membranes, and an ideal scaffold should exhibit properties and an architecture that mimic the extracellular matrix, creating a favorable environment for tissue growth [ 21 , 22 ]. Scaffolds can be created using an electrospinning technique, which is a simple and low-cost method of producing fibers at the micro and nanoscale that generate structures with increased surface area based on the material volume [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO3 Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects

Adolpho

Ribeiro

Freitas

et al. 2023

JFB

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Background: Tissue engineering and cell therapy have been the focus of investigations on how to treat challenging bone defects. This study aimed to produce and characterize a P(VDF-TrFE)/BaTiO3 scaffold and evaluate the effect of mesenchymal stem cells (MSCs) combined with this scaffold and photobiomodulation (PBM) on bone repair. Methods and results: P(VDF-TrFE)/BaTiO3 was synthesized using an electrospinning technique and presented physical and chemical properties suitable for bone tissue engineering. This scaffold was implanted in rat calvarial defects (unilateral, 5 mm in diameter) and, 2 weeks post-implantation, MSCs were locally injected into these defects (n = 12/group). Photobiomodulation was then applied immediately, and again 48 and 96 h post-injection. The μCT and histological analyses showed an increment in bone formation, which exhibited a positive correlation with the treatments combined with the scaffold, with MSCs and PBM inducing more bone repair, followed by the scaffold combined with PBM, the scaffold combined with MSCs, and finally the scaffold alone (ANOVA, p ≤ 0.05). Conclusions: The P(VDF-TrFE)/BaTiO3 scaffold acted synergistically with MSCs and PBM to induce bone repair in rat calvarial defects. These findings emphasize the need to combine a range of techniques to regenerate large bone defects and provide avenues for further investigations on innovative tissue engineering approaches.

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Design and development of biomimetic electrospun sulphonated polyether ether ketone nanofibrous scaffold for bone tissue regeneration applications: in vitro and in vivo study

Cited by 2 publications

References 75 publications

VEGF-modified PLA/HA nanocomposite fibrous membrane for cranial defect repair in rats

VEGF-modified PLA/HA nanocomposite fibrous membrane for cranial defect repair in rats

Mesenchymal Stem Cells Combined with a P(VDF-TrFE)/BaTiO3 Scaffold and Photobiomodulation Therapy Enhance Bone Repair in Rat Calvarial Defects

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