Design and demonstration of multimodal optical scanning microscopy for confocal and two-photon imaging

Chun, Wanhee; Do, Dukho; Gweon, Dae−Gab

doi:10.1063/1.4773232

Cited by 7 publications

(9 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For a conventional laser scanning system [26] with an optics-limited field of view of FOV OL , we can redefine the minimum effective voxel rate, and hence the minimum laser-repetition-rate required for a given laser scanning MPM system to be aliasing-free. Following Equation (3), and considering FOV max = FOV OL , we obtain where, θ ± is the fast-axis scan-angle by the scanning mirror with respect to the optical axis, f o , f s , and f t are the effective focal lengths (EFLs) of the objective lens, scan lens, and tube lens, respectively.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Consequences of the Nyquist-Shannon sampling criterion in Mesoscopic Multiphoton Microscopy to avail full-field sub-micron resolution resolvability

et al. 2021

Preprint

View full text Add to dashboard Cite

With a limited effective voxel rate, to date, each laser-scanning mesoscopic multiphoton microscope (MPM), despite securing an ultra-large field of view (FOV) and an ultra-high optical resolution simultaneously, experiences a fundamental issue with digitization; i.e., inability to satisfy the Nyquist-Shannon sampling criterion to resolve the optics-limited sub-micron resolution over the whole FOV. Such a system either neglects the criterion degrading the digital resolution to twice the pixel size, or significantly reduces the imaging area and/or the imaging speed to respect the digitization. Here we introduce a Nyquist figure of merit parameter to assess this issue, further to comprehend a maximum aliasing-free FOV and a cross-over excitation wavelength for a laser scanning MPM system. Based on our findings we demonstrate an ultra-high voxel rate acquisition in a custom-built mesoscopic MPM system to exceed the Nyquist-rate for a >3800 FOV-resolution ratio while not compromising the imaging speed as well as the photon-budget.

show abstract

Section: Resultsmentioning

confidence: 99%

“…For a conventional laser scanning system [26] with an optics-limited field of view of FOV , we can redefine the minimum effective voxel rate, and hence the minimum laser-repetition-rate required for a given laser scanning MPM system to be aliasing-free. Following Equation (3), and considering FOV = FOV , we obtain…”

Section: Resultsmentioning

confidence: 99%

Consequences of the Nyquist-Shannon sampling criterion in Mesoscopic Multiphoton Microscopy to avail full-field sub-micron resolution resolvability

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…An effective way to simultaneously visualize fibrous and adipose tissue of the liver is to combine MPM, SHG, and hyperspectral microscopy into one imaging platform called multimodal nonlinear optical microscopy [117]. Such an aggregated system has a higher potential for assessing molecular changes than the individual modalities.…”

Section: Promising Methods For Assessing the Structure Function Andmentioning

confidence: 99%

Modern Methods for Assessing the Regenerative Potential of the Liver after Partial Hepatectomy (Review)

Rodimova¹,

Kuznetsova²,

Bobrov³

et al. 2019

Sovrem Tehnol Med

View full text Add to dashboard Cite

The review addresses the main methods for assessing the function and regenerative potential of the liver. They include both the traditional methods, commonly used in clinical practice, and the latest promising techniques suitable for the analysis of cellular and tissue pathology and having a proven diagnostic value.It is known that the dynamics of liver regeneration is reflected in the metabolic status of liver cells, their morphology, and the molecular rearrangement. Therefore, by looking at these parameters we will be able to assess the regenerative potential of the liver as a whole.At present, the most promising method is represented by multiphoton microscopy able to generate the second harmonic; there are also such techniques as coherent anti-stokes Raman spectroscopy (CARS), stimulated Raman scattering (SRS) microscopy, and fluorescence lifetime imaging microscopy (FLIM). In addition, a number of options for analyzing metabolic and structural changes are provided by mass spectrometry, in particular time-of-flight secondary ion mass spectrometry (ToF-SIMS). Studies using these methods with in vivo models and with human biopsy samples demonstrate their relevance in biomedical research and in clinical practice alike.

show abstract

“…The frequency-doubled EDF laser centers around 780 nm, which can be utilized as the excitation source for stainingfree MPM imaging. [25][26][27] Unruh et al 25 and Chun et al 26 used commercial EDF lasers with a frequency-doubled output as the excitation source in benchtop MPM systems, where the fiber lasers were used as a free-space laser source. Recently, a miniature MPM endoscopy system was reported by Huland et al, 27 which utilized a commercial EDF laser with a frequencydoubled output.…”

Section: Introductionmentioning

confidence: 99%

Optimization of frequency-doubled Er-doped fiber laser for miniature multiphoton endoscopy

2018

View full text Add to dashboard Cite

Frequency-doubled femtosecond Er-doped fiber laser is a low-cost and portable excitation source suitable for multiphoton endoscopy. The frequency-doubled wavelength at 780 nm is used to excite the intrinsic fluorescence signal. The frequency-doubling with a periodically poled MgO∶LiNbO 3 (PPLN) is integrated in the distal end of the imaging head to achieve fiber connection. The imaging speed is further improved by optimizing the excitation laser source. A 0.3-mm length of PPLN crystal is selected and the Er-doped fiber laser is manipulated to match its bandwidth with the acceptance bandwidth of the PPLN. Through this optimization, a reduced pulsewidth of 80 fs of the frequency-doubled pulse is achieved. All-fiber dispersion compensation and pulse compression by single mode fiber is conducted, which makes the fiber laser directly fiber-coupled to the imaging head. An imaging speed of 4 frames∕s is demonstrated on ex vivo imaging of unstained biological tissues, which is 10 times faster than our previous study using a 1-mm-long PPLN. The results show that miniature multiphoton endoscopy using frequency-doubled Er-doped fiber laser has great potential for clinical applications. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Design and demonstration of multimodal optical scanning microscopy for confocal and two-photon imaging

Cited by 7 publications

References 22 publications

Consequences of the Nyquist-Shannon sampling criterion in Mesoscopic Multiphoton Microscopy to avail full-field sub-micron resolution resolvability

Consequences of the Nyquist-Shannon sampling criterion in Mesoscopic Multiphoton Microscopy to avail full-field sub-micron resolution resolvability

Modern Methods for Assessing the Regenerative Potential of the Liver after Partial Hepatectomy (Review)

Optimization of frequency-doubled Er-doped fiber laser for miniature multiphoton endoscopy

Contact Info

Product

Resources

About