Design and Control of a Piezo Drill for Robotic Piezo-Driven Cell Penetration

Dai, Changsheng; Sun, Yu; Xin, Liming; Zhang, Zhuoran; Shan, Guanqiao; Wang, Tiancong; Zhang, Kaiwen; Wang, Xian; Chu, Lap-Tak; Ru, Changhai

doi:10.1109/lra.2019.2958734

Cited by 22 publications

(16 citation statements)

References 19 publications

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“…Cell types (such as suspension cells and adherent cells) and cargo properties (such as size, polarity, and morphology) are not limited by this method. The advantages of microinjection technology include precise operation at the micro-nanometer scale (Nadappuram et al, 2019), precise control of injection location and injection volume, high injection success rate, high cell survival rate, and safety (Capecchi, 1980;Dai et al, 2020;Rybouchkin et al, 1995).…”

Section: Individual Injectionmentioning

confidence: 99%

“…2d ). It can automatically detect penetrating cell membrane and stop piezo stack in time to reduce cell damage caused by vibration (Dai et al, 2020). The device is structurally designed to meet standard clinical settings and the piezo stack section has also been improved.…”

Section: Penetrating the Barriersmentioning

confidence: 99%

“…Batch transferring methods include chemical methods (Ali and Ahmed, 2018;Bernkop-Schnürch and Dünnhaupt, 2012;Nguyen et al, 2017;Niu et al, 2019), virus transfection methods (Nayak and Herzog, 2010;Russell and Hirata, 1998;Schaffer et al, 2008;Thomas et al, 2003), and mechanical methods (Kotnik et al, 2015;López-Marín et al, 2018;Mykhaylyk et al, 2007;Peng Pan et al, 2014;Yang et al, 2020;Zhao et al, 2017). Individual cell operation is mainly conducted by microinjection (Dai et al, 2020;Pan et al, 2020;Yoshida and Perry, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Review of Delivering Foreign Substances into Live Cells by Microinjection: Key Technologies and Applications

Chen

Wang

et al. 2022

Preprint

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A key operation in modern genetic engineering is to deliver foreign substances (like modified subcellular organelles, DNA molecules, and drugs) into live cells. Compared with the traditional methods like polyethyleneimine (PEI), cationic liposomes, viral vectors, electroporation, gene gun, etc., microinjection is a rising star due to the advantages of excellent safety, full controllability, high precision, and high survival rate. This review focuses on the recent development of microinjection technology and its applications in animal cells, yeast, fungal cells, and plant cells. Guidelines are provided for selecting the most appropriate microinjection method and system configuration for different application scenarios. When the outer membrane of the host cell is difficult to break, the piezo-driven penetrator could assist the penetration significantly and causes less damage to the host cell during microinjection process. The major existing challenges in microinjection are also analyzed. Overall, microinjection could become a standard technology for modern genetic engineering and microbiology.

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Section: Individual Injectionmentioning

confidence: 99%

Section: Penetrating the Barriersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Review of Delivering Foreign Substances into Live Cells by Microinjection: Key Technologies and Applications

Chen

Wang

et al. 2022

Preprint

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“…In the puncture experiment of mouse oocytes (n=45), the puncture success rate reached 100% and only caused a 3.4 µm deformation of the oocyte during the puncture process. As shown in Figure 9 C , Dai et al 107 installed the piezoelectric actuator next to the holder of the microcapillary by adopting the eccentric configuration of the piezoelectric actuator, which has the advantage of not affecting the installation of the standard microcapillary. At the same time, these authors also developed a technology to correlate filters and motion history images based on angular feature probability data to automatically detect the cell membrane rupture through piezoelectric drilling.…”

Section: Oocyte Micromanipulationmentioning

confidence: 99%

“…(C) Microinjector with piezoelectric actuator eccentric configuration. Adapted with permission from 104 , copyright 2011 Spring Nature, and 106 , copyright 2018 IEEE, and 107 , copyright 2020 IEEE.…”

Section: Figurementioning

confidence: 99%

Recent advances in critical nodes of embryo engineering technology

Ma¹,

Gu²,

Chen³

et al. 2021

Theranostics

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The normal development and maturation of oocytes and sperm, the formation of fertilized ova, the implantation of early embryos, and the growth and development of foetuses are the biological basis of mammalian reproduction. Therefore, research on oocytes has always occupied a very important position in the life sciences and reproductive medicine fields. Various embryo engineering technologies for oocytes, early embryo formation and subsequent developmental stages and different target sites, such as gene editing, intracytoplasmic sperm injection (ICSI), preimplantation genetic diagnosis (PGD), and somatic cell nuclear transfer (SCNT) technologies, have all been established and widely used in industrialization. However, as research continues to deepen and target species become more advanced, embryo engineering technology has also been developing in a more complex and sophisticated direction. At the same time, the success rate also shows a declining trend, resulting in an extension of the research and development cycle and rising costs. By studying the existing embryo engineering technology process, we discovered three critical nodes that have the greatest impact on the development of oocytes and early embryos, namely, oocyte micromanipulation, oocyte electrical activation/reconstructed embryo electrofusion, and the in vitro culture of early embryos. This article mainly demonstrates the efforts made by researchers in the relevant technologies of these three critical nodes from an engineering perspective, analyses the shortcomings of the current technology, and proposes a plan and prospects for the development of embryo engineering technology in the future.

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A Review of Single‐Cell Pose Adjustment and Puncture

Guo

Zhang

Jin

et al. 2022

Advanced Intelligent Systems

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Single‐cell manipulation technology is an essential method of cell research, with wide applications in biological engineering, medical engineering, agricultural engineering, and other precise manipulation fields. Cell pose adjustment and cell puncture are considered as two basic operations of single‐cell manipulation. Cell pose adjustment can be used for cell sorting, cell trapping, cell orientation, cell transportation, etc. It consists of direct contact manipulation methods and noncontact manipulation methods (e.g., mechanical contact method, electric field, optical field, magnetic field, acoustic field, and hydrodynamic methods). Cell puncture consists of ordinary glass needle puncture methods, piezoelectric‐assisted puncture methods, and rotational oscillatory drill puncture methods. It aims to achieve directional and quantified injection or sampling with minimal damage. Herein, the recent research progress on single‐cell pose adjustment and puncture methods is systematically summarized and discussed, which involves the basic mechanism, characteristics, limitations, and applications. Some potential directions for future research are proposed in accordance with the above analysis. It is hoped that this review can provide a reference for academic research and industrial applications of single‐cell manipulation technology.

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Design and Control of a Piezo Drill for Robotic Piezo-Driven Cell Penetration

Cited by 22 publications

References 19 publications

Review of Delivering Foreign Substances into Live Cells by Microinjection: Key Technologies and Applications

Review of Delivering Foreign Substances into Live Cells by Microinjection: Key Technologies and Applications

Recent advances in critical nodes of embryo engineering technology

A Review of Single‐Cell Pose Adjustment and Puncture

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