Design and construction of immune phage antibody library against Tetanus neurotoxin: Production of single chain antibody fragments

Abstract

Our results demonstrated production of functional IgG1 derived from TTX15 scFv and might be an appropriate replacement for polyclonal Tetabulin but it needs further characterization.

Structural basis of tetanus toxin neutralization by native human monoclonal antibodies

“…Numerous reports have described the development of TeNT neutralizing mAbs from either murine or human origin (Fitzsimmons et al, 2000;Scott et al, 2010;Wang et al, 2016). Most, if not all, of the neutralizing mAbs reported could not individually alone completely neutralize the TeNT in vivo (Fitzsimmons et al, 2000;Matsuda et al, 1992;Sadreddini et al, 2015;Scott et al, 2010;Wang et al, 2016;Yousefi et al, 2014bYousefi et al, , 2016 unless high doses of single antibodies were used (Arunachalam et al, 1992;Chin et al, 2003;Kamei et al, 1990;Lang et al, 1993;Volk et al, 1984;Yousefi et al, 2014a). To date, development of a safer and highly efficacious mAb that provides effective protection against tetanus remains a challenging goal.…”

Characterization of neutralizing chimeric heavy-chain antibodies against tetanus toxin

“…Numerous reports have described either murine or human TeNT neutralizing antibodies. 8 , 37 Some of the neutralizing antibodies, when used alone, did not completely neutralize TeNT, but only prolonged the survival of the mice, 8 , 15 , 38 unless used in combination or at high doses. 15 , 39 , 40 The tetanus-neutralizing antibodies 4E12, 3A7, and 4C1, which were developed by Li et al, could completely neutralize 10 × LD 50 using 18 μg of antibody (6 μg each) when used in combination.…”

Developing and characterization of single chain variable fragment (scFv) antibody against frizzled 7 (Fzd7) receptor

“…The titer of outputs and inputs were also determined. 35,47 Five rounds of panning were performed for isolation of specific scFv-phage clones (Fig. 2).…”