Design and characterization of adipose-derived mesenchymal stem cell loaded alginate/pullulan/hyaluronic acid hydrogel scaffold for wound healing applications

Khandan-Nasab, Niloofar; Mahdipour, Elahe; Askarian, Saeede; Kalantari, Mahmoud Reza; Ramezanian, Navid; Oskuee, Reza Kazemi

doi:10.1016/j.ijbiomac.2023.124556

Cited by 17 publications

(7 citation statements)

References 60 publications

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“…ADSC-Ex are small membrane-bound particles secreted by various cell types, including adipose-derived mesenchymal stem cells (ADSCs). ADSC-Ex plays a crucial role in intercellular communication, as they can transfer bioactive molecules, such as proteins, lipids, and nucleic acids, to recipient cells [ 24 ]. In recent years, there has been a growing interest in the therapeutic potential of ADSC-Ex in various diseases, including tissue injury, inflammation, and cancer [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Roles of adipose-derived stem cells and derived exosomes in therapeutic applications to testicular injury caused by cisplatin

Wu,

Lv,

Zheng

et al. 2024

PLoS ONE

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In recent years, adipose-derived stem cells (ADSCs) and derived exosomes (ADSC-Ex) have been investigated for their therapeutic potential in various diseases due to their satisfactory differentiation and regeneration ability. We aimed to explore the potential treatment of ADSCs and ADSC-Ex for testicular injury caused by cisplatin. ADSCs and ADSC-Ex s were identified and extracted to treat the rat model with testicular injury caused by cisplatin. Then the immunohistochemistry and Enzyme linked immunosorbent assay (ELISA) were used to detect the potential treatment of ADSCs and ADSC-Ex. We found that ADSCs and ADSC-Ex significantly improved the testicular tissue damage, increased the number of germ cells, and improved the arrangement of the seminiferous tubules. The levels of malondialdehyde and testosterone were also improved. We speculated that ADSCs and ADSC-Ex may alleviate the testicular injury caused by cisplatin.

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Section: Discussionmentioning

confidence: 99%

Roles of adipose-derived stem cells and derived exosomes in therapeutic applications to testicular injury caused by cisplatin

Wu,

Lv,

Zheng

et al. 2024

PLoS ONE

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“…For instance, hypoxic stimuli were found to enhance the proliferation and growth factor secretion of diabetic ASCs [ 24 , 29 ]. Other attempts included pretreatment with a mitochondrially targeted antioxidant [ 42 ] or lipopolysaccharide [ 43 ], application of ASCs in a SDF-1α gene-activated scaffold [ 44 , 45 ] or hyaluronic acid scaffold [ 15 , 46 ], and combining ASC treatment with PRP [ 6 , 7 , 11 ]. In this study, we discovered that the impaired proliferative and migratory abilities of diabetic ASCs could be partially restored along with decreased senescence by subjecting them to LG culture condition in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…The easy accessibility and low donor site morbidity have made ASCs good candidates for a broad range of cell-based therapeutics. They can be applied alone or in combination with some other biomaterials, such as PRP or hydrogel scaffolds containing hyaluronic acid [ 7 , 11 , 15 ]. Particularly, ASCs secrete several growth factors that stimulate angiogenesis and cell growth, including vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF2) and hepatocyte growth factor (HGF) [ 4 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Low-glucose culture environment can enhance the wound healing capability of diabetic adipose-derived stem cells

Li,

Young,

Wang

et al. 2023

Stem Cell Res Ther

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Background Application of autologous adipose-derived stem cells (ASC) for diabetic chronic wounds has become an emerging treatment option. However, ASCs from diabetic individuals showed impaired cell function and suboptimal wound healing effects. We proposed that adopting a low-glucose level in the culture medium for diabetic ASCs may restore their pro-healing capabilities. Methods ASCs from diabetic humans and mice were retrieved and cultured in high-glucose (HG, 4.5 g/L) or low-glucose (LG, 1.0 g/L) conditions. Cell characteristics and functions were investigated in vitro. Moreover, we applied diabetic murine ASCs cultured in HG or LG condition to a wound healing model in diabetic mice to compare their healing capabilities in vivo. Results Human ASCs exhibited decreased cell proliferation and migration with enhanced senescence when cultured in HG condition in vitro. Similar findings were noted in ASCs derived from diabetic mice. The inferior cellular functions could be partially recovered when they were cultured in LG condition. In the animal study, wounds healed faster when treated with HG- or LG-cultured diabetic ASCs relative to the control group. Moreover, higher collagen density, more angiogenesis and cellular retention of applied ASCs were found in wound tissues treated with diabetic ASCs cultured in LG condition. Conclusions In line with the literature, our study showed that a diabetic milieu exerts an adverse effect on ASCs. Adopting LG culture condition is a simple and effective approach to enhance the wound healing capabilities of diabetic ASCs, which is valuable for the clinical application of autologous ASCs from diabetic patients.

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“…39 Compared to stem cell therapies, exosomes exhibit lower immunogenicity, greater stability, and easier storage. 40,41 Furthermore, exosomes possess the ability to participate in immune regulation, inhibit apoptosis, and promote tissue regeneration, making them a treatment alternative with a favorable performance-risk ratio compared to whole cells. 42,43 The conventional approach involves direct injection of exosomes into the wound site.…”

Section: Introductionmentioning

confidence: 99%

“…Exosomes are extracellular vesicles released by various types of cells that can regulate intercellular communication by delivering their biologically active molecules (nucleic acids, proteins, and lipids) . Compared to stem cell therapies, exosomes exhibit lower immunogenicity, greater stability, and easier storage. , Furthermore, exosomes possess the ability to participate in immune regulation, inhibit apoptosis, and promote tissue regeneration, making them a treatment alternative with a favorable performance-risk ratio compared to whole cells. , The conventional approach involves direct injection of exosomes into the wound site. However, the limited retention and ease of removal hinder their capacity to enhance wound healing.…”

Section: Introductionmentioning

confidence: 99%

Visible-Light Cross-Linkable Multifunctional Hydrogels Loaded with Exosomes Facilitate Full-Thickness Skin Defect Wound Healing through Participating in the Entire Healing Process

Lv,

Li,

Zhang

et al. 2024

ACS Appl. Mater. Interfaces

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The management of severe full-thickness skin defect wounds remains a challenge due to their irregular shape, uncontrollable bleeding, high risk of infection, and prolonged healing period. Herein, an all-in-one OD/GM/QCS@Exo hydrogel was prepared with catecholmodified oxidized hyaluronic acid (OD), methylacrylylated gelatin (GM), and quaternized chitosan (QCS) and loaded with adipose mesenchymal stem cell-derived exosomes (Exos). Cross-linking of the hydrogel was achieved using visible light instead of ultraviolet light irradiation, providing injectability and good biocompatibility. Notably, the incorporation of catechol groups and multicross-linked networks in the hydrogels conferred strong adhesion properties and mechanical strength against external forces such as tensile and compressive stress. Furthermore, our hydrogel exhibited antibacterial, anti-inflammatory, and antioxidant properties along with wound-healing promotion effects. Our results demonstrated that the hydrogel-mediated release of Exos significantly promotes cellular proliferation, migration, and angiogenesis, thereby accelerating skin structure reconstruction and functional recovery during the wound-healing process. Overall, the all-in-one OD/GM/QCS@Exo hydrogel provided a promising therapeutic strategy for the treatment of full-thickness skin defect wounds through actively participating in the entire process of wound healing.

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Design and characterization of adipose-derived mesenchymal stem cell loaded alginate/pullulan/hyaluronic acid hydrogel scaffold for wound healing applications

Cited by 17 publications

References 60 publications

Roles of adipose-derived stem cells and derived exosomes in therapeutic applications to testicular injury caused by cisplatin

Roles of adipose-derived stem cells and derived exosomes in therapeutic applications to testicular injury caused by cisplatin

Low-glucose culture environment can enhance the wound healing capability of diabetic adipose-derived stem cells

Visible-Light Cross-Linkable Multifunctional Hydrogels Loaded with Exosomes Facilitate Full-Thickness Skin Defect Wound Healing through Participating in the Entire Healing Process

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