Design and characterization of a salicylic acid-inducible gene expression system for Jurkat cells

Doshi, Aarti; Bandey, Irfan N; Nevozhay, Dmitry; Varadarajan, Navin; Cirino, Patrick C.

doi:10.1016/j.jbiotec.2022.01.003

Cited by 1 publication

(2 citation statements)

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“…Salicylic acid (SA), which plays a role in plant defense mechanisms against environmental stresses, has been employed to control gene expression in human cells by regulating a bacterial repressor derived from E. coli. [36] Abscisic acid (ABA), also involved in environmental stress management as well as the regulation of growth and development, [35] is widely used in the synthetic biology community to mediate interactions between two ABA-responsive heterodomains (ABI/PYL). [17,37] Vanillic acid (VA) a food additive found in vanilla beans and certain plants, is employed in synthetic biology applications for the regulation of gene circuits at the transcription and posttranslation levels within an orthogonal setup.…”

Section: Plant-derived Compoundsmentioning

confidence: 99%

Section: Small Molecules Commonly Used In Synthetic Biologymentioning

confidence: 99%

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Small‐Molecule Regulators for Gene Switches to Program Mammalian Cell Behaviour

Mansouri,

Fussenegger

2024

ChemBioChem

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Synthetic or natural small molecules have been extensively employed as trigger signals or inducers to regulate engineered gene circuits introduced into living cells in order to obtain desired outputs in a controlled and predictable manner. Here, we provide an overview of small molecules used to drive synthetic‐biology‐based gene circuits in mammalian cells, together with examples of applications at different levels of control, including regulation of DNA manipulation, RNA synthesis and editing, and protein synthesis, maturation, and trafficking. We also discuss the therapeutic potential of these small‐molecule‐responsive gene circuits, focusing on the advantages and disadvantages of using small molecules as triggers, the mechanisms involved, and the requirements for selecting suitable molecules, including efficiency, specificity, orthogonality, and safety. Finally, we explore potential future directions for translation of these devices to clinical medicine.

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