Design and characterization of a porous pouch to prevent peritoneal adhesions during in vivo vascular graft maturation

Shojaee, Mozhgan; Sameti, Mahyar; Vuppuluri, Kranthi; Ziff, Matthew; Carriero, Alessandra; Bashur, Chris A.

doi:10.1016/j.jmbbm.2019.103461

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…[ 19 ] Even though HFIP is toxic, the residual is removed from scaffolds during sterilization and other processing steps, and we have not shown any toxicity of constructs in prior studies. [ 20,21 ]…”

Section: Resultsmentioning

confidence: 99%

“…Cells spreading and density on the different scaffolds were evaluated using fluorescent stains. [ 20 ] Polymer meshes were fixed in 4% w/v paraformaldehyde, permeabilized with 0.1% v/v Triton X‐100 (VWR, Randor, PA), and then blocked with 5% v/v goat serum. Cell nuclei were stained with DAPI contained within the mounting media (Vectashield, Vector Labs, CA), and their cytoskeleton was stained with Alexa 488‐Phalloidin.…”

Section: Methodsmentioning

confidence: 99%

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Reduced Platelet Adhesion for Blended Electrospun Meshes with Low Amounts of Collagen Type I

Sameti

Clarke

Dewan

et al. 2021

Macromolecular Bioscience

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A clinically approved, tissue engineered graft is needed as an alternative for small‐diameter artery replacement. Collagen type I is commonly investigated for naturally derived grafts. However, collagen promotes thrombosis, currently requiring a graft pre‐seeding step. This study investigates unique impacts of blending low collagen amounts with synthetic polymers on scaffold platelet response, which would allow for viable acellular grafts that can endothelialize in vivo. While platelet adhesion and activation are confirmed to be high with 50% collagen samples, low collagen ratios surprisingly exhibit the opposite, anti‐thrombogenic effect. Different platelet interactions in these blended materials can be related to collagen structure. Low collagen ratios show homogenous distribution of the components within individual fibers. Importantly, blended collagen scaffolds exhibit significant differences from gelatin scaffolds, including retaining percentage of collagen after incubation. These findings correlate with functional benefits including better endothelial cell spreading on collagen versus gelatin blended materials. This appears to differ from the current paradigm that processing with harsh solvents will irreversibly denature collagen into less desirable gelatin, but an important distinction is the interaction between collagen and synthetic materials during processing. Overall, excellent anti‐thrombogenic properties of low collagen blends and benefits after grafting show promise for this vascular graft strategy.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Reduced Platelet Adhesion for Blended Electrospun Meshes with Low Amounts of Collagen Type I

Sameti

Clarke

Dewan

et al. 2021

Macromolecular Bioscience

Self Cite

View full text Add to dashboard Cite

show abstract

“…Immunofluorescence staining was performed as in a previous study [26]. Cell nuclei were stained with mounting medium with 4 ′ ,6-diamino-2-phenylindole dihydrochloride (DAPI).…”

Section: Histological and Immunofluorescence Analysismentioning

confidence: 99%

Biomechanical Characterization of Abdominal Aortic Aneurysm: The Rupture Mechanism

Zhai,

Delgado,

Sameti

et al. 2024

Applied Sciences

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In this work, a four-week-old male C57Bl/6 mouse model of abdominal aortic aneurysm (AAA) was developed to examine the AAA rupture mechanism. Immunofluorescence staining was adopted for quantifying the degradation of elastin, and Picrosirius Red staining was adopted for evaluating the density of collagen. Atomic force microscopy with two probe tip sizes of 5 µm and 20 nm was adopted for mechanical characterization of the AAA. The microstructure changes and stiffness changes in both AAA samples and controlled samples were inspected. The degradation of elastin, wall thickening, formation of micro vessels, and increased density of collagen were observed in the AAA samples. The AAA samples also exhibited fragmented texture from AFM scanning. The histogram of stiffness measurements of the AAA samples with a 20 nm tip demonstrated two unique peak frequencies of stiffness intervals (0–10 kPa and 40–50 kPa). The stiffer regions were correlated with the increased density of collagen, as shown in the immunofluorescence images. The softer regions, combined with the fragmented texture, could be the key index contributing to the initiation and propagation of AAA rupture. Overall, the AAA group showed a higher stiffness than the control group (50.77 ± 62.4 kPa vs. 40.6 ± 51.86 kPa). The findings from this work may help in explaining ruptures in small AAA (<5.5 mm), which account for ten percent of all AAA ruptures. Additionally, the observations in this study may help develop early detection methods and innovative treatments for AAA.

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Peritoneal Pre-conditioning Method for In Vivo Vascular Graft Maturation Utilizing a Porous Pouch

Sameti

Bashur

2021

Methods in Molecular Biology

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Design and characterization of a porous pouch to prevent peritoneal adhesions during in vivo vascular graft maturation

Cited by 5 publications

References 28 publications

Reduced Platelet Adhesion for Blended Electrospun Meshes with Low Amounts of Collagen Type I

Reduced Platelet Adhesion for Blended Electrospun Meshes with Low Amounts of Collagen Type I

Biomechanical Characterization of Abdominal Aortic Aneurysm: The Rupture Mechanism

Peritoneal Pre-conditioning Method for In Vivo Vascular Graft Maturation Utilizing a Porous Pouch

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