Design and characterization of a biosensor with lipase immobilized nanoparticles in polymer film for the detection of triglycerides

Protein–polymer bioconjugation, immobilization, and encapsulation: a comparative review towards applicability, functionality, activity, and stability

“…Biomaterials Science or manufacturing biosensors. 208,209 Kumar et al immobilized Lipase T100, a commercial protein, onto silica via physical adsorption. 12 The group employed an approach of cyclic adsorption followed by glutaraldehyde cross-linking.…”

Polymeric Membranes for Biomedical Applications

“…A sensor for triglyceride detection was developed by immobilization of lipase on a polyethyleneimine film (using glutaraldehyde as a linker molecule) deposited on a glassy carbon electrode. The sensor showed a detection limit in the range of 100–500 mg/dL with average recovery values from 95.47 % to 101.05 % [ 250 ]. Introduced by Wulff in 1972 [ 251 ], molecularly imprinted polymers found their applicability in a wide range of biomedical applications, such as the controlled release of drugs or even sensors and biosensors [ 252 ] due to the remarkable properties of molecular or even species with larger dimensions recognition.…”