Design and applications of a novel fluorescent probe for detecting glutathione in biological samples

Label-free colorimetric detection of glutathione by autocatalytic oxidation of o-phenylenediamine based on Au3+ regulation and its application

“…Given that the mechanism of OPD-based sensor is redox reaction, GSH has a better reduction activity than Cys and Hcy; 33 thus, GSH is more likely to respond to the sensing system. Compared with Cys and Hcy, GSH contains more electron-rich group, 38 has a longer distance between the sulfhydryl group and amido group, 39 and has a larger steric hindrance effect; 40 thus, when biological thiols coat the surface of Au NPs and weaken the catalytic effect of Au NPs, GSH can exhibit a better inhibition capability on the autocatalytic reaction.…”

Untitled

“…Thus, we can easily use this probe for glutathione detection in serum samples, nematodes and living cells model. This approach can also be used for designing the other fluorescent probes for GSH detection (Li et al, 2020). Lui et al (2018) reported a heptamethinine cyanine probe (DNIR) with emission and excitation (NIR)excitation 832nm and 804nm, respectively, for the reduced and oxidized glutathione detection (GSH and GSSG) in blood.…”

Use of Fluorescent Probe for the Fast and Quantitative Detection of Chemicals in the Blood

“…Glutathione is a thiol of prime importance present mainly in the form of GSH in the body. Glutathione is a crucial antioxidant within the body (Li et al, 2020). Imbalance of GSH leads to a number of pathologies including Parkinson's disease, cystic fibrosis, HIV, cancer.…”

“…Figure 4: GSH detection in the serum sample, living cell the s and nematodes with CBF probe (Li et al,2020).…”