Design and Application of Receptor-Targeted Fluorescent Probes Based on Small Molecular Fluorescent Dyes

Zhang, Yujie; Li, Shufeng; Zhang, Hang; Xu, Haiwei

doi:10.1021/acs.bioconjchem.0c00606

Cited by 44 publications

(27 citation statements)

References 126 publications

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“…As expected, the highest detection SNR for a given depth was obtained using NIR wavelengths. Most of the recent class of fluorescence guided surgery molecular contrast agents for cancer are NIR, 14 , 41 some of which may ultimately be used as a contrast agent for DiFC. Likewise, circulating fluorescent sensors 7 , 42 can be engineered to emit light in NIR wavelengths.…”

Section: Discussionmentioning

confidence: 99%

Signal and measurement considerations for human translation of diffuse in vivo flow cytometry

et al. 2022

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. Significance: “Diffuse in vivo flow cytometry” (DiFC) is an emerging technology for fluorescence detection of rare circulating cells directly in large deep-seated blood vessels in mice. Because DiFC uses highly scattered light, in principle, it could be translated to human use. However, an open question is whether fluorescent signals from single cells would be detectable in human-scale anatomies. Aim: Suitable blood vessels in a human wrist or forearm are at a depth of to 4 mm. The aim of this work was to study the impact of DiFC instrument geometry and wavelength on the detected DiFC signal and on the maximum depth of detection of a moving cell. Approach: We used Monte Carlo simulations to compute fluorescence Jacobian (sensitivity) matrices for a range of source and detector separations (SDS) and tissue optical properties over the visible and near infrared spectrum. We performed experimental measurements with three available versions of DiFC (488, 640, and 780 nm), fluorescent microspheres, and tissue mimicking optical flow phantoms. We used both computational and experimental data to estimate the maximum depth of detection at each combination of settings. Results: For the DiFC detection problem, our analysis showed that for deep-seated blood vessels, the maximum sensitivity was obtained with NIR light (780 nm) and 3-mm SDS. Conclusions: These results suggest that—in combination with a suitable molecularly targeted fluorescent probes—circulating cells and nanosensors could, in principle, be detectable in circulation in humans.

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Section: Discussionmentioning

confidence: 99%

Signal and measurement considerations for human translation of diffuse in vivo flow cytometry

et al. 2022

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“…As expected, the highest detected signal to noise ratio for a given depth was obtained using NIR wavelengths. Most of the recent class of fluorescence guided surgery molecular contrast agents for cancer are NIR 13,38 , some of which may ultimately be used as a contrast agent for DiFC. Likewise, circulating fluorescent sensors 7,39 can be engineered to emit light in NIR wavelengths.…”

Section: Discussionmentioning

confidence: 99%

Signal and Measurement Considerations for Human Translation of Diffuse in vivo Flow Cytometry

Ivich

Pace

Williams

et al. 2022

Preprint

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SignificanceDiffuse in vivo flow cytometry (DiFC) is an emerging technology for fluorescence detection of rare circulating cells directly in large deep-seated blood vessels in mice. Because DiFC uses highly scattered light, in principle it could be translated to human use. However, an open question is whether fluorescent signals from single cells would be detectable in human-scale anatomies.AimSuitable blood vessels in a human wrist or forearm are at a depth of approximately 2-4 mm. The aim of this work was to study the impact of DiFC instrument geometry and wavelength on the detected DiFC signal and on the maximum depth of detection of a moving cell.ApproachWe used Monte Carlo simulations to compute Jacobian (sensitivity) matrices for a range of source-detector separations and tissue optical properties over the visible and near infrared (NIR) spectrum. We performed experimental measurements with three available versions of DiFC (488 nm, 640 nm, and 780 nm), fluorescent microspheres, and tissue mimicking optical flow phantoms. We used both computational and experimental data to estimate the maximum depth of detection at each combination of settings.Results and ConclusionsFor the DiFC detection problem, our analysis showed that for deep-seated blood vessels, the maximum sensitivity was obtained with NIR light (780 nm) and 3 mm source-and-detector separation. These results suggest that - in combination with a suitable molecularly targeted fluorescent probes - circulating cells and nanosensors could in principle be detectable in circulation in humans.

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“…The fluorescent molecules have been widely applied in various areas including analyzing proteins, immunoassays, visual recognition, efficient isolation of cells, etc. [ 1 , 2 , 3 , 4 , 5 ]. The selective labeling of such molecules onto proteins or other biomolecules draws attention, accompanied with the development of bioorthogonal chemistry, including Staudinger ligation and click chemistry, which also enables molecules with fluorescence to be applied as reporter groups [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Fluorescent Properties of Aminopyridines and the Application in “Click and Probing”

Chang

et al. 2022

Molecules

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Unsubstituted pyridin-2-amine has a high quantum yield and is a potential scaffold for a fluorescent probe. However, the facile access to conjugated highly substituted aminopyridines and the study of their fluorescent properties is scarce. In this paper, synthesis and fluorescent properties of multisubstituted aminopyridines were studied based on a recently developed Rh-catalyzed coupling of vinyl azide with isonitrile to form a vinyl carbodiimide intermediate, following tandem cyclization with an alkyne. An aminopyridine substituted with an azide group as a potential probe was further designed, synthesized, and evaluated. The “clicking-and-probing” experiment of it on BSA protein showed the potential of aminopyridine as a scaffold of a biological probe.

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Design and Application of Receptor-Targeted Fluorescent Probes Based on Small Molecular Fluorescent Dyes

Cited by 44 publications

References 126 publications

Signal and measurement considerations for human translation of diffuse in vivo flow cytometry

Signal and measurement considerations for human translation of diffuse in vivo flow cytometry

Signal and Measurement Considerations for Human Translation of Diffuse in vivo Flow Cytometry

Synthesis and Fluorescent Properties of Aminopyridines and the Application in “Click and Probing”

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