Design and analysis of a microfluidic lab-on-chip utilizing dielectrophoresis mechanism for medical diagnosis and liquid biopsy

Pakhira, Writtick; Kumar, Rajesh; Ibrahimi, Khalid Mohd; Bhattacharjee, Rituraj

doi:10.1007/s40430-022-03793-4

Cited by 7 publications

(7 citation statements)

References 27 publications

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“…Technologies based on the dielectrophoresis effects are effective for the analysis of fatigue, force, and stress at the cellular level. For example, cell manipulation systems have been widely employed to measure cellular biomechanics using microfluidic platforms, including studies on cell stretching and manipulation [135,136], electrical property changes of stored RBC [137], label-free and noninvasive characterization for the viscoelastic properties of RBC [138],benchmarking dielectrophoretic separation metrics of unknown types of RBC (healthy, modified, …) [139], the oxidative stress analysis for RBCs (Figure 9A) [140], dynamic fatigue measurements [141], detecting circadian rhythms in RBCs [142], nonlinear viscoelastic analyses (Figure 9B) [143], biomechanics of erythrocyte membrane failures [144], liquid metal electrode-based dielectrophoretic schemes [145], and a portable system with multiple dielectrophoretic applications for RBC analyses [146].…”

Section: Rbc Dielectrophoretic Analysismentioning

confidence: 99%

Section: Rbc Dielectrophoretic Analysismentioning

confidence: 99%

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Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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The utilizations of microfluidic chips for single RBC (red blood cell) studies have attracted great interests in recent years to filter, trap, analyze, and release single erythrocytes for various applications. Researchers in this field have highlighted the vast potential in developing micro devices for industrial and academia usages, including lab-on-a-chip and organ-on-a-chip systems. This article critically reviews the current state-of-the-art and recent advances of microfluidics for single RBC analyses, including integrated sensors and microfluidic platforms for microscopic/tomographic/spectroscopic single RBC analyses, trapping arrays (including bifurcating channels), dielectrophoretic and agglutination/aggregation studies, as well as clinical implications covering cancer, sepsis, prenatal, and Sickle Cell diseases. Microfluidics based RBC microarrays, sorting/counting and trapping techniques (including acoustic, dielectrophoretic, hydrodynamic, magnetic, and optical techniques) are also reviewed. Lastly, organs on chips, multi-organ chips, and drug discovery involving single RBC are described. The limitations and drawbacks of each technology are addressed and future prospects are discussed.

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Section: Rbc Dielectrophoretic Analysismentioning

confidence: 99%

Section: Rbc Dielectrophoretic Analysismentioning

confidence: 99%

Advances in Microfluidics for Single Red Blood Cell Analysis

et al. 2023

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“…Platelet separation and purification are required in a variety of medical applications ranging from the detection and treatment of hemorrhagic and thrombotic diseases to blood transfusions [ 1 , 2 ]. Low platelet concentration can cause hemorrhaging, whereas high concentration can lead to thrombosis and related complications such as infarction, embolism, or stroke.…”

Section: Introductionmentioning

confidence: 99%

Continuous Flow Separation of Red Blood Cells and Platelets in a Y-Microfluidic Channel Device with Saw-Tooth Profile Electrodes via Low Voltage Dielectrophoresis

Hewlin

Edwards

2023

CIMB

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Cell counting and sorting is a vital step in the purification process within the area of biomedical research. It has been widely reported and accepted that the use of hydrodynamic focusing in conjunction with the application of a dielectrophoretic (DEP) force allows efficient separation of biological entities such as platelets from red blood cell (RBC) samples due to their size difference. This paper presents computational results of a multiphysics simulation modelling study on evaluating continuous separation of RBCs and platelets in a microfluidic device design with saw-tooth profile electrodes via DEP. The theoretical cell particle trajectory, particle cell counting, and particle separation distance study results reported in this work were predicted using COMSOL v6.0 Multiphysics simulation software. To validate the numerical model used in this work for the reported device design, we first developed a simple y-channel microfluidic device with square “in fluid” electrodes similar to the design reported previously in other works. We then compared the obtained simulation results for the simple y-channel device with the square in fluid electrodes to the reported experimental work done on this simple design which resulted in 98% agreement. The design reported in this work is an improvement over existing designs in that it can perform rapid separation of RBCs (estimated 99% purification) and platelets in a total time of 6–7 s at a minimum voltage setting of 1 V and at a minimum frequency of 1 Hz. The threshold for efficient separation of cells ends at 1000 kHz for a 1 V setting. The saw-tooth electrode profile appears to be an improvement over existing designs in that the sharp corners reduced the required horizontal distance needed for separation to occur and contributed to a non-uniform DEP electric field. The results of this simulation study further suggest that this DEP separation technique may potentially be applied to improve the efficiency of separation processes of biological sample scenarios and simultaneously increase the accuracy of diagnostic processes via cell counting and sorting.

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“…Blood plays a crucial role in the transport of substances within the human body and contains a variety of cells, such as red blood cells, white blood cells, lymphocytes, or circulating tumor cells [1]. The quantity, type, morphology, and ratio of different cell populations are intricately linked to the overall state of an individual's health [2]. For example, low platelet concentration can lead to bleeding, whereas high concentration can lead to thrombosis, stroke, and other diseases [3].…”

Section: Introductionmentioning

confidence: 99%

Numerical simulation on performance improvement for blood cell separation under sheath-assisted dielectrophoresis

Hu,

Wang,

Tong

2023

Preprint

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Dielectrophoresis (DEP), known as an attractive and frugal technique, can be used to manipulate biological or non-biological particles in microfluidics. This paper presents a three-dimensional sheath-assisted microfluidic chip for focusing cells and separating red blood cells (RBCs) from white blood cells (WBCs) in continuous flow. Based on the control variables, a simulation model using COMSOL Multiphysics 6.0 is calculated to obtain the favorable flow rate ratio under an electric potential as low as 14 Vpp, at the frequency of 175 kHz. Both RBCs and WBCs respond to negative dielectrophoresis forces and the performance of the separation process are analyzed by evaluating the purity and separation efficiency. The results reveal that the optimal flow rate ratio of the device is suitable to effectively separate RBCS from WBCs with high purity and cell separation efficiency factors up to 88% and 97%, at the throughput of 8 µL/h. The current research provides valuable insights into the design of microchip devices for the effective and selective separation regarding different cells in biological applications.

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Design and analysis of a microfluidic lab-on-chip utilizing dielectrophoresis mechanism for medical diagnosis and liquid biopsy

Cited by 7 publications

References 27 publications

Advances in Microfluidics for Single Red Blood Cell Analysis

Advances in Microfluidics for Single Red Blood Cell Analysis

Continuous Flow Separation of Red Blood Cells and Platelets in a Y-Microfluidic Channel Device with Saw-Tooth Profile Electrodes via Low Voltage Dielectrophoresis

Numerical simulation on performance improvement for blood cell separation under sheath-assisted dielectrophoresis

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