Design and analysis of a burst strength device for testing vascular grafts

Pérez-Aranda, C.; Gamboa, F.; Castillo-Cruz, O.; Cauich-Rodríguez, Juan Valerio; Avilés, F.

doi:10.1063/1.5037578

Cited by 5 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The testing device that provides the measurements of burst pressure and compliance is designed and developed in cooperation with the company (Inovenso, Turkey) in accordance with ISO 7198:2016 standard . The burst strength of the vascular graft is described as the pressure at which it fails, and circumferential compliance, which is used as a flexibility or stiffness metric, is expressed as the percentage of diameter change for every 100 mmHg (%/100 mmHg) . Normally, in the case of textile fabrics, the burst strength analysis is often applied in a planar form .…”

Section: Methodsmentioning

confidence: 99%

Optimization of Electrospun Bilayer Vascular Grafts through Assessment of the Mechanical Properties of Monolayers

Ozdemir,

Oztemur,

Sezgin

et al. 2024

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Small-diameter vascular grafts must be obtained with the most appropriate materials and design selection to harmoniously display a variety of features, including adequate tensile strength, compliance, burst strength, biocompatibility, and biodegradability against challenging physiological and hemodynamic conditions. In this study, monolayer vascular grafts with randomly distributed or radially oriented fibers are produced using neat, blended, and copolymer forms of polycaprolactone (PCL) and poly(lactic acid) (PLA) via the electrospinning technique. The blending ratio is varied by increasing 10 in the range of 50−100%. Bilayer graft designs are realized by determining the layers with a random fiber distribution for the inner layer and radial fiber orientation for the outer layer. SEM analysis, wall thickness and fiber diameter measurements, tensile strength, elongation, burst strength, and compliance tests are done for both mono-and bilayer scaffolds. The findings revealed that the scaffolds made of neat PCL show more flexibility than the neat PLA samples, which possess higher tensile strength values than neat PCL scaffolds. Also, in blended samples, the tensile strength values do not show a significant improvement, whereas the elongation values are enhanced in tubular samples, depending on the blending ratio. Also, neat poly(L-lactide-co-caprolactone) (PLCL) samples have both higher elongation and strength values than neat and blended scaffolds, with some exceptions. The blended specimens comprising a combination of PCL and PLA, with blending ratios of 80/20 and 70/30, exhibited the most elevated burst pressures. Conversely, the PLCL scaffolds demonstrated superior compliance levels. These findings suggest that the blending approach and fiber orientation offer enhanced burst strength, while copolymer utilization in PLCL scaffolds without fiber alignment enhances their compliance properties. Thus, it is evident that using a copolymer instead of blending PCL and PLA and combining the PLCL layer with PCL and PLA monolayers in bilayer vascular graft design is promising in terms of mechanical and biological properties.

show abstract

Section: Methodsmentioning

confidence: 99%

Optimization of Electrospun Bilayer Vascular Grafts through Assessment of the Mechanical Properties of Monolayers

Ozdemir,

Oztemur,

Sezgin

et al. 2024

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…The burst strength test was conducted using a custom-made piece of equipment designed and manufactured for this type of test [41], as shown in Figure 2c. An 80 mm long vascular graft was pressurized (0.3 psi/s, ~15.5 mmHg/s, for all tests herein) using compressed air.…”

Section: Burst Strengthmentioning

confidence: 99%

A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method

Zumbardo-Bacelis,

Peponi,

Vargas-Coronado

et al. 2024

Polymers

View full text Add to dashboard Cite

This study used the roto-evaporation technique to engineer a 6 mm three-layer polyurethane vascular graft (TVG) that mimics the architecture of human coronary artery native vessels. Two segmented polyurethanes were synthesized using lysine (SPUUK) and ascorbic acid (SPUAA), and the resulting materials were used to create the intima and adventitia layers, respectively. In contrast, the media layer of the TVG was composed of a commercially available polyurethane, Pearlbond 703 EXP. For comparison purposes, single-layer vascular grafts (SVGs) from individual polyurethanes and a polyurethane blend (MVG) were made and tested similarly and evaluated according to the ISO 7198 standard. The TVG exhibited the highest circumferential tensile strength and longitudinal forces compared to single-layer vascular grafts of lower thicknesses made from the same polyurethanes. The TVG also showed higher suture and burst strength values than native vessels. The TVG withstood up to 2087 ± 139 mmHg and exhibited a compliance of 0.15 ± 0.1%/100 mmHg, while SPUUK SVGs showed a compliance of 5.21 ± 1.29%/100 mmHg, akin to coronary arteries but superior to the saphenous vein. An indirect cytocompatibility test using the MDA-MB-231 cell line showed 90 to 100% viability for all polyurethanes, surpassing the minimum 70% threshold needed for biomaterials deemed cytocompatibility. Despite the non-cytotoxic nature of the polyurethane extracts when grown directly on the surface, they displayed poor fibroblast adhesion, except for SPUUK. All vascular grafts showed hemolysis values under the permissible limit of 5% and longer coagulation times.

show abstract

“…Both components are positioned at an angle of 30 with respect to the perpendicular axis of the incidence plane. 13 This test allowed direct measurement of burst strength from the average of at least five specimens where the burst strength was defined as the maximum internal pressure at which the SDVGs burst.…”

Section: Burst Strengthmentioning

confidence: 99%

On arginine‐based polyurethane‐blends specific to vascular prostheses

Zumbardo-Bacelis

Meza-Villegas

Pérez-Aranda

et al. 2021

J of Applied Polymer Sci

View full text Add to dashboard Cite

Polymer blends based on Tecoflex™ and an experimental aliphatic polyurethane (HMDI-PCL-arginine stands for 4,4 (metylenebiscyclohexyl) isocyanate -poly (ε caprolactone) diol, SPUUR stands for segmented poly(urea)urethanes using amino acid of L-Arginine as chain extender) were obtained by solvent casting, and further studied by fourier transform infrared (FTIR) and Raman spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis, and X-ray diffraction (XRD). Their biological performances were assessed in terms of hemocompatibility and Human umbilical vein endothelial cell (HUVEC) cytotoxicity. Tensile properties of dumbbell specimens were compared to longitudinal and circumferential tensile properties of tubular vascular graft. FTIR showed that as the SPUUR content increased in the blend, absorptions at 2860 cm À1 increased, carbonyl absorptions at 1724 cm À1 broaden and the small peak at 2796 cm À1 , typical of Tecoflex™ disappeared. Raman spectroscopy showed that the low intensity carbonyl absorption at 1724 cm À1 also increased with SPUUR content. DSC allowed detection of PCL soft segment melting (T m = 50 C) in agreement with X-ray reflections at 21.3 and 23.6 , assigned to SPUUR. However, no improvements in thermal stability were detected by TGA by blending. The addition of SPUUR to Tecoflex™ improved hemocompatibility and HUVEC cytotoxicity. The vascular grafts performance showed that 40% SPUUR blends exhibited the highest force in the longitudinal test whereas 50% SPUUR blends showed the highest circumferential force. Pressure burst strength was higher than 1000 mmHg for all blends. Overall, these blends can be used for high caliber vascular grafts.

show abstract

Design and analysis of a burst strength device for testing vascular grafts

Cited by 5 publications

References 26 publications

Optimization of Electrospun Bilayer Vascular Grafts through Assessment of the Mechanical Properties of Monolayers

Optimization of Electrospun Bilayer Vascular Grafts through Assessment of the Mechanical Properties of Monolayers

A Comparison of Three-Layer and Single-Layer Small Vascular Grafts Manufactured via the Roto-Evaporation Method

On arginine‐based polyurethane‐blends specific to vascular prostheses

Contact Info

Product

Resources

About