Design and analysis of a squamous cell carcinoma in vitro model system

Abstract

Tissue-engineered skin equivalents based on primary isolated fibroblasts and keratinocytes have been shown to be useful tools for functional in vitro tests, including toxicological screenings and drug development. In this study, a commercially available squamous cell carcinoma (SCC) cell line SCC-25 was introduced into epidermal and full-thickness skin equivalents to generate human-based disease-in-a-dish model systems. Interestingly, when cultured either in the epidermis or dermis of full-thickness skin equivalents, SCC-25 cells formed hyper-keratinized tumor cell nests, a phenomenon that is frequently seen in the skin of patients afflicted with SCC. Raman spectroscopy was employed for the label-free cell phenotype characterization within the engineered skin equivalents and revealed the presence of differential protein patterns in keratinocytes and SCC-25 cells. To conclude, the here presented SSC disease-in-a-dish approaches offer the unique opportunity to model SSC in human skin in vitro, which will allow further insight into SSC disease progression, and the development of therapeutic strategies.

Cell death stages in single apoptotic and necrotic cells monitored by Raman microspectroscopy

“…To selectively collect and analyze Raman spectra of viable cells (Annexin V-FITC − /PI − population), early apoptotic cells (Annexin V-FITC + /PI − ), late apoptotic cells (Annexin V-FITC + /PI + ) and heat-treated primary necrotic cells (Annexin V-FITC + /PI + ), spectral acquisition and fluorescence imaging was combined. Raman spectroscopy and imaging of Annexin V-FITC and PI fluorescence were conducted simultaneously on stained cells using our custom-built Raman microspectrometer system825262728. Cellular uptake of the fluorescent dyes did not impact the Raman spectral profiles (Supplemental Figure S2).…”

FT-IR Spectroscopy Study in Early Diagnosis of Skin Cancer

“…Sun UV and ionizing radiation are responsible for sun burns, premature aging, and skin carcinogenesis. The mechanisms of skin carcinogenesis have been extensively studied (1)(2)(3)(4)(5)(6). Basal cell carcinoma (BCC), squamous-cell carcinoma (SCC), and skin melanoma (MM) are the three main types of skin cancer (7).…”

Organ-On-A-Chip: Development and Clinical Prospects Toward Toxicity Assessment with an Emphasis on Bone Marrow

“…In this context, many studies have focused on generating organ-on-a-chip models of the liver and kidney due to the importance of theses organs in drug metabolism and clearance [13][14][15]. Models of other important organs, e.g., lung [16,17], skin [18][19][20], and the gastrointestinal (GI) tract [21,22], have also been developed for toxicity testing, since those organs are closely related to allergic and immunogenic disorders caused by exogenous substances [23,24].…”