Design and 3D Printing of Hydrogel Scaffolds with Fractal Geometries

Warner, John J.; Soman, Pranav; Zhu, Wei; Tom, Matthew; Chen, Shaochen

doi:10.1021/acsbiomaterials.6b00140

Cited by 88 publications

(72 citation statements)

References 42 publications

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“…Namely, digital micro-mirror device projection printing (DMD-PP) is a stereolithographic method that uses an array of micro-mirrors to spatially modulate a pulsed UV laser, in order to achieve additive crosslinking patterns in a photo-curable material. [17,[39][40][41][42] Similarly, it can also be used for subtractive laser ablation within previously crosslinked layers of the material. An alternative approach is to 3D print a sacrificial mold (a.k.a.…”

Section: Discussionmentioning

confidence: 99%

An Automated Addressable Microfluidics Device for Minimally Disruptive Manipulation of Cells and Fluids within Living Cultures

Tong

Pham

Shah

et al. 2019

Preprint

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According to the U.S. Department of Health & Human Services, nearly 115,000 people in the U.S needed a lifesaving organ transplant in 2018, while only ~10% of them have received it. Yet, almost no artificial FDA-approved products are commercially available todaythree decades after the inception of tissue engineering. It is hypothesized that the major bottlenecks restricting its progress stem from lack of access to the inner pore space of the scaffolds. Specifically, the inability to deliver nutrients to, and clear waste from, the center of the scaffolds limits the size of the products that can be cultured. Likewise, the inability to monitor, and control, the cells after seeding them into the scaffold results in nonviable tissue, with an unacceptable product variability. To resolve these bottlenecks, we present a prototype addressable microfluidics device capable of minimally-invasive fluid and cell manipulation within living cultures. As proof-of-concept, we demonstrate its ability to perform additive manufacturing by seeding cells in spatial patterns (including co-culturing multiple cell types); and subtractive manufacturing, by removing surface adherent cells via targeted trypsin release. Additionally, we show that the device is capable of sampling fluids and performing cell "biopsies" (which can be subsequently sent for ex-situ testing), from any location within its culture chamber. Finally, the on-chip plumbing is completely automated using external electronics. This opens up the possibility to perform long-term computer-driven tissue engineering experiments, where the cell behavior is modulated in response to the minimally-invasive observations (e.g. fluid sampling and cell biopsies) throughout the whole duration of the cultures. It is expected that the proofof-concept technology will eventually be scaled up to 3D addressable microfluidic scaffolds, capable of overcoming the limitations bottlenecking the transition of tissue engineering technologies to the clinical setting.

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Section: Discussionmentioning

confidence: 99%

An Automated Addressable Microfluidics Device for Minimally Disruptive Manipulation of Cells and Fluids within Living Cultures

Tong

Pham

Shah

et al. 2019

Preprint

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“…The third method for 3D-bioprinting is Light-Based Bioprinting (LBB), also commonly referred to as laser-based bioprinting. LBB, which includes laser-assisted printing and stereolithography, configures as the fastest and most resolute method among all bioprinting strategies, with no limitations associated with the material viscosity [ 34 , 178 , 179 ].…”

Section: Bioprinting Processmentioning

confidence: 99%

Advances on Bone Substitutes through 3D Bioprinting

Genova

Roato

Carossa

et al. 2020

IJMS

101

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Reconstruction of bony defects is challenging when conventional grafting methods are used because of their intrinsic limitations (biological cost and/or biological properties). Bone regeneration techniques are rapidly evolving since the introduction of three-dimensional (3D) bioprinting. Bone tissue engineering is a branch of regenerative medicine that aims to find new solutions to treat bone defects, which can be repaired by 3D printed living tissues. Its aim is to overcome the limitations of conventional treatment options by improving osteoinduction and osteoconduction. Several techniques of bone bioprinting have been developed: inkjet, extrusion, and light-based 3D printers are nowadays available. Bioinks, i.e., the printing materials, also presented an evolution over the years. It seems that these new technologies might be extremely promising for bone regeneration. The purpose of the present review is to give a comprehensive summary of the past, the present, and future developments of bone bioprinting and bioinks, focusing the attention on crucial aspects of bone bioprinting such as selecting cell sources and attaining a viable vascularization within the newly printed bone. The main bioprinters currently available on the market and their characteristics have been taken into consideration, as well.

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“…[ 277,278 ] Compared to conventional maskless photolithography, projection‐based stereolithography (PSL) offers the advantages of increased throughput and complexity, and precise control over scaffold thickness and feature resolution. [ 279,280 ] As proof‐of‐concept, hydrogel‐encapsulated ECs were spatially positioned in single or multiple layers within defined geometries which resulted in correlated vascular geometries with a mature phenotype and cord‐like structures. [ 281 ] Cell‐laden hydrogels surrounded by multiple perfusable lumenized vascular channels have also been fabricated using this technique.…”

Section: Fabrication Strategies To Generate Microfluidic and Vascularmentioning

confidence: 99%

Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology

Pradhan

Banda

Farino

et al. 2020

Adv Healthcare Materials

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The vascular system is integral for maintaining organ‐specific functions and homeostasis. Dysregulation in vascular architecture and function can lead to various chronic or acute disorders. Investigation of the role of the vascular system in health and disease has been accelerated through the development of tissue‐engineered constructs and microphysiological on‐chip platforms. These in vitro systems permit studies of biochemical regulation of vascular networks and parenchymal tissue and provide mechanistic insights into the biophysical and hemodynamic forces acting in organ‐specific niches. Detailed understanding of these forces and the mechanotransductory pathways involved is necessary to develop preventative and therapeutic strategies targeting the vascular system. This review describes vascular structure and function, the role of hemodynamic forces in maintaining vascular homeostasis, and measurement approaches for cell and tissue level mechanical properties influencing vascular phenomena. State‐of‐the‐art techniques for fabricating in vitro microvascular systems, with varying degrees of biological and engineering complexity, are summarized. Finally, the role of vascular mechanobiology in organ‐specific niches and pathophysiological states, and efforts to recapitulate these events using in vitro microphysiological systems, are explored. It is hoped that this review will help readers appreciate the important, but understudied, role of vascular‐parenchymal mechanotransduction in health and disease toward developing mechanotherapeutics for treatment strategies.

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Design and 3D Printing of Hydrogel Scaffolds with Fractal Geometries

Cited by 88 publications

References 42 publications

An Automated Addressable Microfluidics Device for Minimally Disruptive Manipulation of Cells and Fluids within Living Cultures

An Automated Addressable Microfluidics Device for Minimally Disruptive Manipulation of Cells and Fluids within Living Cultures

Advances on Bone Substitutes through 3D Bioprinting

Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology

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