Design and 3D Printing of Scaffolds and Tissues

An, Jia; Teoh, Joanne Ee Mei; Suntornnond, Ratima; Chua, Chee Kai

doi:10.15302/j-eng-2015061

Cited by 383 publications

(237 citation statements)

References 81 publications

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“…A new term known as 3D bioprinting has emerged recently and researchers are working towards the realization of printing functional human organs with this novel technology. An et al [20] reviewed vastly on various state-of-the-art 3D printing technologies for tissue engineering applications, limitations of the current technologies and the possible future improvements. Electrohydrodynamic Jetting, which is also known as EHD-Jetting or E-jetting is one type of bioprinting technology.…”

Section: Introductionmentioning

confidence: 99%

Investigation of process parameters of electrohydrodynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries

Wang¹,

Vijayavenkataraman²,

Wu³

et al. 2024

IJB

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Abstract:Tissue engineering is a promising technology in the field of regenerative medicine with its potential to create tissues de novo. Though there has been a good progress in this field so far, there still exists the challenge of providing a 3D micro-architecture to the artificial tissue construct, to mimic the native cell or tissue environment. Both 3D printing and 3D bioprinting are looked upon as an excellent solution due to their capabilities of mimicking the native tissue architecture layer-by-layer with high precision and appreciable resolution. Electrohydrodynamic jetting (E-jetting) is one type of 3D printing, in which, a high electric voltage is applied between the extruding nozzle and the substrate in order to print highly controlled fibres. In this study, an E-jetting system was developed in-house for the purpose of 3D printing of fibrous scaffolds. The effect of various E-jetting parameters, namely the supply voltage, solution concentration, nozzle-to-substrate distance, stage (printing) speed and solution dispensing feed rate on the diameter of printed fibres were studied at the first stage. Optimized parameters were then used to print Polycaprolactone (PCL) scaffolds of highly complex geometries, i.e., semi-lunar and spiral geometries, with the aim of demonstrating the flexibility and capability of the system to fabricate complex geometry scaffolds and biomimic the complex 3D micro-architecture of native tissue environment. The spiral geometry is expected to result in better cell migration during cell culture and tissue maturation.

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Section: Introductionmentioning

confidence: 99%

Investigation of process parameters of electrohydrodynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries

Wang¹,

Vijayavenkataraman²,

Wu³

et al. 2024

IJB

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show abstract

“…3D printing technology has addressed these problems by leveraging the magnetic resonance imaging (MRI) and computerized tomography (CT) data of patients . Due to its controllability and manufacturing capability, 3D printing has grown to be a definite part of tissue engineering during the second decade after its birth …”

Section: Introductionmentioning

confidence: 99%

Composite PLA/PEG/nHA/Dexamethasone Scaffold Prepared by 3D Printing for Bone Regeneration

Wang

et al. 2018

Macromolecular Bioscience

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3D printing has become an essential part of bone tissue engineering and attracts great attention for the fabrication of bioactive scaffolds. Combining this rapid manufacturing technique with chemical precipitation, biodegradable 3D scaffold composed of polymer matrix (polylactic acid and polyethylene glycol), ceramics (nano hydroxyapatite), and drugs (dexamethasone (Dex)) is prepared. Results of water contact angle, differential scanning calorimeter, and mechanical tests confirm that incorporation of Dex leads to significantly improved wettability, higher crystallinity degree, and tunable degradation rates. In vitro experiment with mouse MC3T3-E1 cells implies that Dex released from scaffolds is not beneficial for early cell proliferation, but it improves late alkaline phosphatase secretion and mineralization significantly. Anti-inflammation assay of murine RAW 264.7 cells proves that Dex released from all the scaffolds successfully suppresses lipopolysaccharide induced interleukin-6 and inducible nitric oxide synthase secretion by M1 macrophages. Further in vivo experiment on rat calvarial defects indicates that scaffolds containing Dex promote osteoinduction and osteogenic response and would be promising candidates for clinical applications.

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“…These ways include developing new materials for use with 'traditional' AM processes, as well as developing technology and devices that use existing materials such as the paste-like materials of interest in our research, in an AM process. In regards to the latter, advances have been made in 3D printing materials that had been previously restricted to conventional manufacturing processes, such as scaffolds for tissue engineering [3], cell cultures [4], live tissue [5], food [6], medical implants [7], ceramics [8], cements [9], and composites [10] with the use of specially designed paste extruders. It is therefore important that AM equipment keeps up with these new developments.…”

Section: Introductionmentioning

confidence: 99%

Paste Extruder—Hardware Add-On for Desktop 3D Printers

2017

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This paper presents the design, development and testing of a paste/clay extrusion device intended to be used as a drop-in replacement for the conventional thermoplastic extruder of a desktop filament-based 3D printer. A plastic cylinder loaded with gel, paste or clay material is placed into the device. Feedstock is pressed through an extrusion nozzle by a piston driven by an electrically actuated drive-screw and nut mechanism. The device allows the build material to heat up to 80 • C. Forced air cooling is used to assist the cooling or hardening process of the freshly-printed material during fabrication. The feedstock container, nozzle, and material-loading process are all suitable for use in a sterile environment. The device is designed for seamless integration with existing 3D printing firmware and slicing software. After designing the device, a prototype was produced and installed on a 3D printer. Silicone and acrylic polymers, as well as dental gel, were used to fabricate 3D printed sample objects.

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Design and 3D Printing of Scaffolds and Tissues

Cited by 383 publications

References 81 publications

Investigation of process parameters of electrohydrodynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries

Investigation of process parameters of electrohydrodynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries

Composite PLA/PEG/nHA/Dexamethasone Scaffold Prepared by 3D Printing for Bone Regeneration

Paste Extruder—Hardware Add-On for Desktop 3D Printers

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