Desiccation tolerance of rhizobia when protected by synthetic polymers

Deaker, Rosalind; Roughley, R. J.; Kennedy, Ivan R.

doi:10.1016/j.soilbio.2006.09.005

Cited by 38 publications

(38 citation statements)

References 11 publications

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“…Previous studies have shown that slow-growing rhizobia such as CB1809 are less susceptible to desiccation stress than the fast-growing strains of rhizobia such as TA1 (Bushby and Marshall, 1977b;Deaker et al, 2007). This may explain the lack of significant improvement in survival of CB1809 as this strain of rhizobia has an inherently greater capacity for desiccation tolerance than TA1.…”

Section: General Discussion and Conclusionmentioning

confidence: 99%

“…Previous studies have shown that slowgrowing rhizobia such as bradyrhizobia are less susceptible to desiccation stress than the fastgrowing strains of rhizobia such as R. leguminosarum sp. (Bushby and Marshall, 1977b;Deaker et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Water suspensions of cells were used to determine desiccation tolerance. Desiccation tolerance of cells grown in different media was determined using a modified vacuum drying technique as described by Deaker et al (2007).…”

Section: Desiccation Tolerance Of Rhizobia Grown In Different Culturementioning

confidence: 99%

“…PVA was selected as an external protectant as it has been demonstrated in the past to better protect desiccated cells of rhizobia than other polymers such as methyl cellulose and polyvinyl pyrrolidone (Deaker et al, 2007). Survival of rhizobia in their study was found to be optimised at a minimum of 5% PVA in the vacuum drying system.…”

Section: Desiccation Tolerance Of Rhizobiamentioning

confidence: 99%

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The Physiological Mechanisms of Desiccation Tolerance in Rhizobia

Casteriano¹,

Wilkes²,

Deaker³

2015

Biological Nitrogen Fixation

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Section: General Discussion and Conclusionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Desiccation Tolerance Of Rhizobia Grown In Different Culturementioning

confidence: 99%

Section: Desiccation Tolerance Of Rhizobiamentioning

confidence: 99%

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The Physiological Mechanisms of Desiccation Tolerance in Rhizobia

Casteriano¹,

Wilkes²,

Deaker³

2015

Biological Nitrogen Fixation

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“…In order to increase the inoculant quality and effi ciency, and to reduce costs and environmental impacts, alternative carrier materials have been studied (Ben Rebah et al, 2007;Albareda et al, 2008), including single and composite polymer formulations (Dommergues et al, 1979;Jawson et al, 1989;Denardin & Freire, 2000;Schuh, 2005;Deaker et al, 2007), which have already been evaluated as rhizobial carriers (Dommergues et al, 1979;Denardin & Freire, 2000;Sarr et al, 2005), and associative bacteria (Bashan & Gonzales, 1999).…”

Section: Introductionmentioning

confidence: 99%

Polymers as carriers for rhizobial inoculant formulations

Fernandes‐Júnior

Rohr

Oliveira

et al. 2009

Pesq. agropec. bras.

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-The aim of this work was to evaluate the effi ciency of carboxymethyl cellulose (CMC) and starch blends as carrier materials of rhizobial inoculants regarding their capacity to maintain viable cells and promote cowpea (Vigna unguiculata) nodulation. The experimental design adopted was completely randomized, with three replicates. Forty different compositions of carboxymethyl cellulose (CMC) with starch, compatibilized or not with different proportions of MgO or ZnO, were evaluated regarding their ability of maintaining rhizobial viable cells during the storage period of one month at room temperature, in an initial screening. Thereafter, selected inoculant carrier blends were evaluated regarding their ability to maintain viable rhizobial cells for a period of 165 days, and their performance as inoculant carriers was compared to a peat-based inoculant carrier under greenhouse conditions. Rhizobial cells were better maintained in blends containing 50-60% CMC. Compatibilizing agents did not increase survival of rhizobial cells for 30 days of storage. The cowpea nodulation of polymer blends was statistically the same of peat-based inoculants. CMC/starch polymer blends are effi cient carriers to rhizobial inoculants for up to 165 days of storage, when compatibilized with MgO (1%).Index terms: Bradyrhizobium japonicum, Vigna unguiculata, biological nitrogen fi xation, carboxymethyl cellulose, inoculant technology, polymer blends. Polímeros como veículos para formulações de inoculantes rizobianosResumo -O objetivo deste trabalho foi avaliar a efi ciência de misturas poliméricas de carboximetilcelulose (CMC) e amido, como veículos de inoculante para rizóbios, quanto à sua capacidade de manter células rizobianas viáveis e promover a nodulação em feijão-caupi (Vigna unguiculata). O delineamento experimental foi completamente casualizado, com três repetições. Quarenta diferentes composições poliméricas de carboximetilcelulose (CMC) e amido, compatibilizadas ou não com proporções de MgO ou ZnO, foram inicialmente avaliadas quanto à sua capacidade de manter células rizobianas viáveis pelo período de um mês. Posteriormente, veículos de inoculantes selecionados foram avaliados quanto à capacidade de manter células rizobianas viáveis pelo período de 165 dias, e seu desempenho como veículos de inoculantes foi comparado com os de inoculantes turfosos, em casa de vegetação. Células rizobianas sobreviveram melhor em misturas com 50-60% de CMC. Os agentes compatibilizantes não aumentaram a sobrevivência das células rizobianas após 30 dias de estocagem. A nodulação do feijão-caupi com o uso das misturas poliméricas não diferiu estatisticamente da nodulação com o uso da turfa. As misturas de CMC/amido, quando compatibilizadas com MgO (1%), são veículos efi cientes para inoculantes rizobianos por até 165 dias de armazenamento.Termos para indexação: Bradyrhizobium japonicum, Vigna unguiculata, fi xação biológica de nitrogênio, carboximetilcelulose, tecnologia de inoculantes, misturas poliméricas.

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