Desiccation tolerance, longevity and seed-siring ability of entomophilous pollen from UK native orchid species

Marks, T. R.; Seaton, Philip T.; Pritchard, Hugh W.

doi:10.1093/aob/mcu139

Cited by 22 publications

(20 citation statements)

References 33 publications

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“…Wang et al ( 2015 ) observed that the pollen in litchi showed the better quantity in the filaments extended farther before the anther cracked. Germination increased to 80% in Anacamptis morio at the beginning of anthesis (Marks et al 2014 ), at which time the moisture of fresh pollen remains higher. The pollen germinability was affected not only by the anther dehiscence but also the pollen age (Bellusci et al 2010 ; Marks et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

et al. 2018

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BackgroundIn a breeding program, usually only superior parents are chosen for cross hybridization. Pollens of elite cultivars may not be available at hand. Properly stored pollens provide an opportunity for cross hybridization at unavailable time.ResultsPollen of a Phalaenopsis hybrid was evaluated for the storage ability at different temperatures, including room temperature, 4, − 20, and − 80 °C for up to 96 weeks. The viability of pollen was assessed by TTC staining, in vitro germination and hand pollination during and after storage. Pollen stored at all temperatures for 4 weeks remained viable and capable of successful pollination. Pollen lost its viability after 4 weeks at room temperature. Pollen remains viable after 40 weeks at 4 °C, and after 96 weeks at both − 20 and − 80 °C of storage. Viable pollen could be successfully pollinated to the female parent at all effective storage conditions and produced seeds.ConclusionsOur results indicate that Phalaenopsis pollen can be stored at 4 °C up to 40 weeks for short-term purpose. For long-term storage, pollen can be kept at both − 20 and −80 °C.

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Section: Discussionmentioning

confidence: 99%

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

et al. 2018

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“…In this protocol, the tested bryophytes were classified into two groups according to their survival at equilibration at either 67 or 23% RH (Wood ). With regards to other groups of DT organisms, different desiccation procedures have been used as a method to discriminate recovery levels, including lichens (Fernández‐Marín et al , Gauslaa et al ), free‐living microalgae (Candotto Carniel et al ), fern gametophytes (Riaño and Briones ) and angiosperm pollen (Marks et al ).…”

Section: Introductionmentioning

confidence: 99%

A field portable method for the semi‐quantitative estimation of dehydration tolerance of photosynthetic tissues across distantly related land plants

López‐Pozo

Flexas

Gulías

et al. 2019

Physiologia Plantarum

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Desiccation tolerant (DT) plants withstand complete cellular dehydration, reaching relative water contents (RWC) below 30% in their photosynthetic tissues. Desiccation sensitive (DS) plants exhibit different degrees of dehydration tolerance (DHT), never surviving water loss >70%. To date, no procedure for the quantitative evaluation of DHT extent exists that is able to discriminate DS species with differing degrees of DHT from truly DT plants. We developed a simple, feasible and portable protocol to differentiate between DT and different degrees of DHT in the photosynthetic tissues of seed plants and between fast desiccation (< 24 h) tolerant (FDT) and sensitive (FDS) bryophytes. The protocol is based on (1) controlled desiccation inside Falcon tubes equilibrated at three different relative humidities that, consequently, induce three different speeds and extents of dehydration and (2) an evaluation of the average percentage of maximal photochemical efficiency of PSII (F v /fm) recovery after rehydration. Applying the method to 10 bryophytes and 28 tracheophytes from various locations, we found that (1) imbibition of absorbent material with concentrated salt-solutions inside the tubes provides stable relative humidity and avoids direct contact with samples; (2) for 50 ml capacity tubes, the optimal plant amount is 50-200 mg fresh weight; (3) the method is useful in remote locations due to minimal instrumental requirements; and (4) a threshold of 30% recovery of the initial F v /fm upon reaching RWC ≤ 30% correctly categorises DT species, with three exceptions: two poikilochlorophyllous species and one gymnosperm. The protocol provides a semi-quantitative expression of DHT that facilitates comparisons of species with different morpho-physiological traits and/or ecological attributes.

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“…Ovule and pollen longevity is an important factor in determining the ability to produce seeds. The life span of ovule and pollen is species specific and adapted to its ecological requirements [ 20 , 38 ]. Ethylene has been reported to regulate the onset of ovule senescence [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis GPR1 Gene Negatively Affects Pollen Germination, Pollen Tube Growth, and Gametophyte Senescence

Yang

Zhang

Zhao

et al. 2017

IJMS

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Genes essential for gametophyte development and fertilization have been identified and studied in detail; however, genes that fine-tune these processes are largely unknown. Here, we characterized an unknown Arabidopsis gene, GTP-BINDING PROTEIN RELATED1 (GPR1). GPR1 is specifically expressed in ovule, pollen, and pollen tube. Enhanced green fluorescent protein-tagged GPR1 localizes to both nucleus and cytoplasm, and it also presents in punctate and ring-like structures. gpr1 mutants exhibit no defect in gametogenesis and seed setting, except that their pollen grains are pale in color. Scanning electron microscopy analyses revealed a normal patterned but thinner exine on gpr1 pollen surface. This may explain why gpr1 pollen grains are pale. We next examined whether GPR1 mutation affects post gametogenesis processes including pollen germination, pollen tube growth, and ovule senescence. We found that gpr1 pollen grains germinated earlier, and their pollen tubes elongated faster. Emasculation assay revealed that unfertilized gpr1 pistil expressed the senescence marker PBFN1:GUS (GUS: a reporter gene that encodes β-glucuronidase) one-day earlier than the wild type pistil. Consistently, ovules and pollen grains of gpr1 mutants showed lower viability than those of the wild type at 4 to 5 days post anthesis. Together, these data suggest that GPR1 functions as a negative regulator of pollen germination, pollen tube growth, and gametophyte senescence to fine-tune the fertilization process.

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Desiccation tolerance, longevity and seed-siring ability of entomophilous pollen from UK native orchid species

Abstract: Substantial pollen desiccation tolerance and life span of the four entomophilous orchids reflects a resilient survival strategy in response to unpredictable pollinator visitation, and presents an alternative approach to germplasm conservation.

Cited by 22 publications

References 33 publications

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

A field portable method for the semi‐quantitative estimation of dehydration tolerance of photosynthetic tissues across distantly related land plants

The Arabidopsis GPR1 Gene Negatively Affects Pollen Germination, Pollen Tube Growth, and Gametophyte Senescence

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