Desferrioxamine E produced by Streptomyces griseus stimulates growth and development of Streptomyces tanashiensis

Yamanaka, Kazuhiro; Oikawa, Hiroaki; Ogawa, Hiro-Omi; Hosono, Kuniaki; Shinmachi, Fumie; Takano, Hideaki; Sakuda, Shohei; Beppu, Teruhiko; Uéda, Kenji

doi:10.1099/mic.0.28139-0

Cited by 159 publications

(138 citation statements)

References 28 publications

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“…28,29 Taken together, these results demonstrate that inhibition of development and antibiotic production by physiological signalling can also be circumvented by potentiating siderophore-mediated iron uptake in S. coelicolor. [9][10][11] Transcription of iron-and development-related genes in bldA, bldJ and bldK mutants…”

Section: S Coelicolor Bld Mutants Are Affected In Siderophore Biosynmentioning

confidence: 99%

“…formation of aerial hyphae and spores) and secondary metabolite production was triggered in certain species when they were grown in proximity to isolates producing siderophores (more precisely desferrioxamine E, DFOE). 9 This was the first observation suggesting that in Streptomyces species, these ironchelating compounds might have a much more complex and subtle role in triggering a wide range of cellular processes. 6,9 In line with these findings, Tierrafria and collaborators showed that the disruption of the desferrioxamine-binding receptor encoded by desE resulted in loss of sporulation in S. coelicolor.…”

Section: Introductionmentioning

confidence: 99%

“…9 This was the first observation suggesting that in Streptomyces species, these ironchelating compounds might have a much more complex and subtle role in triggering a wide range of cellular processes. 6,9 In line with these findings, Tierrafria and collaborators showed that the disruption of the desferrioxamine-binding receptor encoded by desE resulted in loss of sporulation in S. coelicolor. 10 Recently, co-culture experiments demonstrated that iron competition through siderophore piracy between actinomycetes prevented S. coelicolor from entering its sporulation program.…”

Section: Introductionmentioning

confidence: 99%

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Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

et al. 2014

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Streptomyces coelicolor is an important model organism for developmental studies of filamentous GCrich actinobacteria. The genetic characterization of mutants of S. coelicolor blocked at the vegetative mycelium stage, the so-called bald (bld) mutants that are unable to erect spore-forming aerial hyphae, has opened the way to discovering the molecular basis of development in actinomycetes. Desferrioxamine (DFO) production and import of ferrioxamines (FO; iron-complexed DFO) are key to triggering morphogenesis of S. coelicolor and we show here that growth of S. coelicolor on the reference medium for Streptomyces developmental studies is fully dependent on DFO biosynthesis. UPLC-ESI-MS analysis revealed that all bld mutants tested are affected in DFO biosynthesis, with bldA, bldJ, and ptsH mutants severely impaired in DFO production, while bldF, bldK, crr and ptsI mutants overproduce DFO.Morphogenesis of bldK and bldJ mutants was recovered by supplying exogenous iron. Transcript analysis showed that the bldJ mutant is impaired in expression of genes involved in the uptake of FO, whereas transcription of genes involved in both DFO biosynthesis and FO uptake is increased in bldK mutants. Our study allows proposing altered DFO production and/or FO uptake as a novel phenotypic marker of many S. coelicolor bld mutants, and strengthens the role of siderophores and iron acquisition in morphological development of actinomycetes.

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Section: S Coelicolor Bld Mutants Are Affected In Siderophore Biosynmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

et al. 2014

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“…It appears likely to be that a diffusible substance produced by S. griseus compensated for a certain deficiency in S. tanashiensis. The promotive factor was successfully isolated from the pure liquid culture of S. griseus and was identified as desferrioxamine E. 24 Desferrioxamines are a group of siderophores often produced by Streptomyces. In a natural environment, the availability of iron to cells is quite limited, because of its insolubility.…”

Section: Desferrioxaminesmentioning

confidence: 99%

Antibiotics in microbial coculture

Uéda

Beppu

2016

J Antibiot

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Today, the frequency of discovery of new antibiotics in microbial culture is significantly decreasing. The evidence from whole-genome surveys suggests that many genes involved in the synthesis of unknown metabolites do exist but are not expressed under conventional cultivation conditions. Therefore, it is urgently necessary to study the conditions that make otherwise silent genes active in microbes. Here we overview the knowledge on the antibiotic production promoted by cocultivation of multiple microbial strains. Accumulating evidence indicates that cocultivation can be an effective way to stimulate the production of substances that are not formed during pure cultivation. Characterization of the promotive factors produced by stimulator strains is expected to give clues to the development of effective cultivation conditions for drug discovery. The Journal of Antibiotics (2017) 70, 361-365; doi:10.1038/ja.2016; published online 19 October 2016 INTRODUCTIONEver since the discovery of penicillin, antibiotics and many other kinds of biologically active substances have been obtained from microbial cultures. The divergent actions of these compounds have contributed to the current knowledge of the basic mechanisms of molecular interaction and therapeutic methods as well. This special issue contains studies dealing with the milestone works that have advanced the development of basic biological sciences and medicinal applications.In contrast to the great successes in mining of useful natural products in the last century, the frequency of isolation of new compounds from microbial cultures is substantially decreasing in this century. 1 In accordance with this situation, the pharmaceutical industry is changing the direction of drug development and reducing the weight of natural products among the resources for screening.The factors limiting the chances of new discovery can be attributed to humans, not to microorganisms. Studies on the accumulating genomic information indicate that a large number of biosynthetic gene clusters are not expressed under the conventional culture conditions. 1,2 Namely, the potential diversity of microbial products is still large, but scientists do not yet know how to fully take advantage of the abilities of microbes. This problem is due in part to the fact that the methods of modern microbiology still depend on the manipulation techniques developed by Robert Koch and colleagues, which relies on the single-colony isolation from solid agar plates and subsequent pure cultivation under laboratory conditions. We can imagine that the dependence on those artificial conditions restricts access to a number of microorganisms and/or their abilities.To address the issue, attempts have been made to discover new substances specifically produced in coculture. In natural environments, microbes are living in diverse associations with other organisms. The modes of interaction include antagonism, commensalism and mutualism. Functions specifically correlating with such interactions may not get activated during conv...

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“…We recently identified desferrioxamines, which represent a class of siderophores widely produced by Streptomyces, as an example of such metabolites. 4 In this study, we explore another interspecific stimulatory event involved in antibiotic production (antibiosis). Structural analyses of a newly isolated antibiosis-promoting substance revealed that this substance is a polyether antibiotic structurally related to lonomycin and mutalomycin.…”

Section: Introductionmentioning

confidence: 99%

Promomycin, a polyether promoting antibiotic production in Streptomyces spp.

et al. 2010

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Widespread interspecific stimulation of antibiotic production occurs in strains of Streptomyces owing to the activity of diffusible substances, as previously determined in our investigations of the cross-feeding effect. In this study, we newly isolated a substance produced by a Streptomyces strain closely related to Streptomyces scabrisporus, based on the observation that this substance induced the production of an unknown antibiotic in another strain related to Streptomyces griseorubiginosus. This substance, named promomycin, is a polyether structurally related to lonomycin. Promomycin itself had an antibiotic activity, but it stimulated antibiotic production in multiple Streptomyces strains at sub-inhibitory concentrations. Evidence implies that this stimulation effect is widespread within this group of bacteria. The Journal of Antibiotics (2010) Keywords: antibiotic production; ionophore; polyether; promomycin; Streptomyces INTRODUCTION Antibiotic production in Streptomyces is affected by various environmental stimuli. 1 Accumulating evidence has shown that specific diffusible metabolites in addition to physicochemical stimuli induce antibiotic production in Streptomyces. Such metabolites include gbutyrolactone autoregulators, which are the best-characterized hormonal signals in Streptomyces and are widely distributed among members of this genus. Since the discovery of A-factor (2-isocapryloyl 3R-hydroxymethy-g-butyrolactone), secondary metabolism in Streptomyces has been shown to be generally controlled by this type of signal produced by the organism itself. 2 Both structural diversity and ligandreceptor specificity indicate that g-butyrolactone sensing occurs in an intraspecific manner, rather than in an interspecific manner.In contrast to the concept of autoregulation, our previous observations have shown that interspecific stimulation of secondary metabolism and/or morphogenesis takes place among various Streptomyces species and other related bacteria. 3 Cross-feeding assays on solid media (see Figure 1a as a reference) have shown that an array of colonies of one strain (corresponding to strain 574 in Figure 1a) responded to a concentration gradient of a substance diffusing from the colony of another strain (corresponding to strain 153 in Figure 1a). To determine whether such stimulatory events between different species involve unknown specific metabolites, we have attempted to isolate the metabolites responsible for the stimulatory activity. We recently identified desferrioxamines, which represent a

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Desferrioxamine E produced by Streptomyces griseus stimulates growth and development of Streptomyces tanashiensis

Cited by 159 publications

References 28 publications

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor

Antibiotics in microbial coculture

Promomycin, a polyether promoting antibiotic production in Streptomyces spp.

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