Desenvolvimento e caracterização de sistemas de liberação tópica a base de cristais líquidos com vitamina E TPGS para veiculação de siRNA na terapia gênica

Mano, Danielle de Macedo

doi:10.11606/d.60.2012.tde-27062013-093226

Cited by 2 publications

(1 citation statement)

References 76 publications

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“…Two scales were used. A first dilution was prepared using 1mL of cationic formulation, previously obtained with polyethylenimine (PEI) by Mano [28] and using 9mL of sterile distilled water, in order to make the first dilution (1:10 dilution -scale x). This 1:10 sample was submitted to 100 manual agitations, so as to produce the first decimal dilution (1x).…”

Section: Preparation Of the High Dilutionsmentioning

confidence: 99%

Cationic topical formulations tested in high dilutions offers protective effects in fibroblast cells culture

Mano¹,

Oliveira²,

Leonel

et al. 2021

Int J High Dilution Res

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Topical administration is a simple and comfortable form of cutaneous administration of drugs. However, in this route of administration the drug needs to overcome the barrier posed by the skin to reach an effective concentration. For this reason, many topical formulations are developed with a cationic component. The promotion of absorption occurs due to the disruption of the stratum corneum. But this cationic component has also high irritating potential to the skin. The biotherapics are medicines prepared from a toxic product or etiologic agent, following the homeopathic pharmacopoeia technique, and they are used mainly in cases of hipersensitization. In this experiment, high dilutions (HD) obtained from a cationic formulation were prepared and evaluated considering cell viability in â€œin vitroâ€ mouse fibroblast (L929) culture cells model by a colorimetric MTT assay. No signs of toxicity were observed, which demonstrates the safety of these HD preparations to the healthy cells. The effectiveness of these HD was also investigated in cells damaged by cationic formulations. The results demonstrated that the HD 30c was the most effective preparation in preventing the cell damage caused by the tested irritating product, increasing cell viability from 56.6% (damaged cells) to 100% (similar to negative control group, p>0.05). These results provide evidence of the positive action of high dilutions against the exposure to a cytotoxic agent.

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Section: Preparation Of the High Dilutionsmentioning

confidence: 99%

Cationic topical formulations tested in high dilutions offers protective effects in fibroblast cells culture

Mano¹,

Oliveira²,

Leonel

et al. 2021

Int J High Dilution Res

View full text Add to dashboard Cite

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Sistemas líquido-cristalinos de monoleína e água para veiculação de antimicrobianos com aplicabilidade bucal

Souza¹

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Desenvolvimento e caracterização de sistemas de liberação tópica a base de cristais líquidos com vitamina E TPGS para veiculação de siRNA na terapia gênica

Abstract: MANO, D. M. Development and characterization of topical delivery systems based on liquid crystals with vitamin E TPGS for siRNA in gene. 2012. 101 f. Dissertation (Master).

Cited by 2 publications

References 76 publications

Cationic topical formulations tested in high dilutions offers protective effects in fibroblast cells culture

Cationic topical formulations tested in high dilutions offers protective effects in fibroblast cells culture

Sistemas líquido-cristalinos de monoleína e água para veiculação de antimicrobianos com aplicabilidade bucal

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