Desenvolvimento dos fungos Metarhizium anisopliae (Metschnikoff, 1879) Sorokin, 1883 E Beauveria bassiana (Balsamo) Vuillemin, 1912 sobre Ctenocephalides felis felis (Bouché, 1835)

Abstract

The flea Ctenocephalides felis felis is a parasite that causes allergic dermatitis and also may transmit etiologic agents to domestic animals and humans. This study investigated by scanning electron microscopy the development of entomopathogenic fungi on flea cuticle. Fleas were exposed to conidia (10(8) ml(-1)) of Metarhizium anisopliae (isolate 959) or Beauveria bassiana (isolate 986). Following standard protocols for electron microscopy, the specimens were prepared 2, 15, 26 and 96 h after infection. The micrography revealed that 2 h after fungus exposure, conidia attachments encompassed the entire flea cuticle, especially on abdominal intersegmental membranes. The emergence of germ tubes and appressoria formation occurred at 15 h, thickening and branching of hyphae on the flea cuticle was noted at 26 h. Therefore, both of these fungal isolates were able to develop on cuticular surfaces of C. f. felis.

Prokaryotic Expression, Purification, and Functional Characterization of the Large Yellow Croaker (Larimichthys crocea) Mannose Receptors Subunits (MRC1 and MRC2)

“…Entomopathogenic fungi (EPF) represent a biocontrol alternative that has been documented in various ectoparasites of veterinary importance both in vitro and in the field conditions; the presence of EPF in the insects under natural conditions has also been demonstrated (Fernandes et al, 2012;Galindo-Velasco et al, 2015;Cruz-Vázquez et al, 2015). In the case of fleas, there are antecedents in which they have been shown to be susceptible to infection by Beauveria bassiana (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Hypocreales: Clavicipitaceae), under laboratory conditions (De Melo et al, 2007 and their presence within insect under natural conditions (Ortega-Palomares et al, 2014). The fungi isolated from an insect may be specifically adapted as biological control agents of the same insect (Fernandes and Bittencourt, 2008), to our knowledge, there is only one report of the occurrence of EPF in C. canis (Ortega-Palomares et al, 2014).…”