Desensitization in the Innervated and in the Chronically Denervated Soleus Muscle of the Mouse

Hall, Moira T.; Maleque, M. A.; Wadsworth, R. M.

doi:10.1111/j.1476-5381.1975.tb07620.x

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…The addition of acetylcholine to isolated chronically denervated soleus muscles results in biphasic contractions (Hall, Maleque & Wadsworth, 1975). The second phase of tension is reduced when the extracellular calcium concentration is lowered and increased when [Ca2 ] is raised.…”

Section: Introduction Methodsmentioning

confidence: 99%

“…The addition of acetylcholine to isolated chronically denervated soleus muscles results in biphasic contractions (Hall, Maleque & Wadsworth, 1975 probably terminated by a process of contractile inactivation. It was therefore concluded that activator calcium for the second phase comes from a source in equilibrium with the extracellular fluid, while activator calcium for the first phase is derived from an intracellular source (Hall, Maleque & Wadsworth, 1977).…”

Section: Introduction Methodsmentioning

confidence: 99%

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The Effects of Glycerol Detubulation, of Manganese Ions, of Dantrolene and of Nitrate Ions on the Responses of Isolated Chronically Denervated Soleus Muscles of the Mouse to Acetylcholine

Hall

Maleque

Wadsworth

1978

British J Pharmacology

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In the isolated chronically denervated soleus muscle of the mouse acetylcholine produced a biphasic contraction. In muscles detubulated by glycerol treatment, the first phase of the acetylcholine contraction was almost abolished, but the second was only slightly reduced or was unaffected. Manganese ions (10 mM) reduced the first phase of the acetylcholine response, but enhanced the second. Dantrolene sodium (5.94 times 10−5 M) reduced the first phase of the acetylcholine response, but not the second. Nitrate ions (118 mM) augmented the first phase but not the second. It was concluded that the first phase requires the excitation‐contraction‐coupling sequence: membrane depolarization‐T‐tubules‐Ca2+ release from sarcoplasmic reticulum. In contrast, the second phase appears to occur independently of these processes.

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Section: Introduction Methodsmentioning

confidence: 99%

Section: Introduction Methodsmentioning

confidence: 99%

The Effects of Glycerol Detubulation, of Manganese Ions, of Dantrolene and of Nitrate Ions on the Responses of Isolated Chronically Denervated Soleus Muscles of the Mouse to Acetylcholine

Hall

Maleque

Wadsworth

1978

British J Pharmacology

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“…There is a double response which consists of a fast initial contraction associated with a burst of fibrillary potentials followed by a slow contracture and the abolition of fibrillation (Brown, 1937;Rosenblueth & Luco, 1937;Bowman & Raper, 1964). Chronically denervated muscle has usually been reported to produce single phase contractions when isolated (Elmqvist & Thesleff, 1960;Letley, 1960;Freeman & Turner, 1969;Lullmann & Sunano, 1973), but biphasic responses have been obtained in the chronically denervated and isolated diaphragm (Preuner, 1971;Liillman, Preuner & Schaube, 1974) and soleus muscle (Hall, Maleque & Wadsworth, 1975).…”

Section: Introductionmentioning

confidence: 99%

The Role of Extracellular Calcium in the Contractions Produced by Acetylcholine in Chronically Denervated Muscle

Hall¹,

Maleque²,

Wadsworth³

1977

British J Pharmacology

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I Acetylcholine-induced contractions of the isolated chronically denervated soleus muscle of the mouse consist of two phases, but both phases are equivalent to the contracture phase seen in vivo.2 Low [Ca2+J0 (0.5-1.5 mM) augmented peak tension, as well as the rate of relaxation, of the first phase, but inhibited the second phase. Ethyleneglycol-bis4p-aminoethyl ether)-N,NM-tetraacetic acid (EGTA) or La3+ (2 mM) also inhibited the second phase, but not the first. 3 It was concluded that the first phase requires Ca2+ release from the sarcoplasmic reticulum, and is terminated by inactivation of the contractile process. The second phase is caused by the entry of activator Ca2+ from the extracellular space.4 Increasing [Ca2+]o to 5 or 10 mm after the addition of acetylcholine caused a contraction, starting after a delay of about 50 seconds. EGTA or La3+ added during the second phase of the acetylcholine contraction caused relaxation after a much shorter lag time. 5 It is concluded that most of the Ca2+ entering from the extracellular fluid is taken up by the sarcoplasmic reticulum. 6 The acetylcholine second phase was augmented in low (25 mM) [Na+I0. It is concluded that Na+ and Ca2+ compete for the acetylcholine controlled ionic channels. 7 Isolated chronically denervated diaphragm muscles were less sensitive to acetylcholine and the contraction usually consisted of a first phase only. 8 It is concluded that sequestration of Ca2+ entering from the extracellular fluid is more complete in the diaphragm.

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Force and membrane potential in acetylcholine and potassium contractures of denervated mouse muscles

Lorković

1985

Pflugers Arch.

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Depolarization and contracture force (P) provoked by acetylcholine (ACh) and by K ions were studied in bundles dissected from mouse soleus muscles that had been denervated for 4-7 days. Cl-free solutions were used. The muscle fibres were depolarized by solutions containing 150 mM K or 10 microM ACh to nearly zero mV resulting in maximum P (Pmax). Threshold P was produced when the membrane was depolarized to more than about -60 mV by both agents. 50% Pmax was produced by [K] causing the membrane to depolarize to -42 mV, whereas a potential more positive than -20 mV was required for 50% Pmax to be produced when ACh was used. The rate of depolarization was always higher for ACh than for K. Pretreatment by 0.05 microM ACh (about threshold for P) did not affect the P-[K] relation appreciably showing that ACh did not "stabilize" the membrane. Nearly equal P was provoked by successive applications of just suprathreshold agent concentrations when the order of application was ACh----K but not with the reverse order. Hypertonicity (by addition of 300 mM sucrose to all solutions) caused PACh to decrease and PK to increase. It was concluded that the ACh receptors are located in the surface membrane of the muscle fibres, not in the T-system membranes.

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Desensitization in the Innervated and in the Chronically Denervated Soleus Muscle of the Mouse

Cited by 4 publications

References 25 publications

The Effects of Glycerol Detubulation, of Manganese Ions, of Dantrolene and of Nitrate Ions on the Responses of Isolated Chronically Denervated Soleus Muscles of the Mouse to Acetylcholine

The Effects of Glycerol Detubulation, of Manganese Ions, of Dantrolene and of Nitrate Ions on the Responses of Isolated Chronically Denervated Soleus Muscles of the Mouse to Acetylcholine

The Role of Extracellular Calcium in the Contractions Produced by Acetylcholine in Chronically Denervated Muscle

Force and membrane potential in acetylcholine and potassium contractures of denervated mouse muscles

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