Desensitization and recovery of crayfish photoreceptors. Dependency on circadian time, and pigment-dispersing hormone

Barriga-Montoya, Carolina; O-Martínez, Araceli de la; Fuentes‐Pardo, Beatriz; Gómez-Lagunas, Froylán

doi:10.1016/j.cbpa.2016.10.008

Cited by 5 publications

(8 citation statements)

References 57 publications

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“…Notice the transient character of the transduction currents, which reach a maximum, peak, amplitude in tens of milliseconds and thereafter decay slowly, following an exponential time course. 1,2 See that, as expected, the trace corresponding to the flash of higher intensity presents bigger amplitude (peak value) and faster activation kinetics, whereas the decay, inactivation, phase is not appreciably changed at these light intensities. Time between stimulus was 2 min to allow full recovery from inactivation.…”

Section: Kinetics Of Crayfish Photoreceptor: Light Intensity Dependencesupporting

confidence: 70%

“…Time between stimulus was 2 min to allow full recovery from inactivation. 1,2,3 These features are best seen in the Figure inset which shows the normalized, superposed, currents. Interestingly, applying a series of light pulses of varying intensity we noticed that kinetics of the light transduction-current, activated by a lightpulse, depends not only on the activating pulse itself, but, unexpectedly, also depends on whether the prior pulse had a higher or a lower intensity than that of the activating light-pulse.…”

Section: Kinetics Of Crayfish Photoreceptor: Light Intensity Dependencementioning

confidence: 88%

“…Additionally, and importantly, notice that the fitted curves cross at both the lowest and highest intensity flash-values: this demonstrate that the observed behavior is not due to incomplete recovery from inactivation. 2 In order to assess the magnitude of the hysteresis response shown in Figure 2, we measured the area between each pair of exponential curves that fit the flash-intensity dependence of the parameters that characterize the light-triggered current (see Materials and Methods). The latter is illustrated in Fig 3A which shows the area (AL, shaded region) between the curves that describe the variation of current latency (L) as a function of light intensity.…”

Section: Kinetics Of Crayfish Photoreceptor: Light Intensity Dependencementioning

confidence: 99%

“…As photoreceptors are involved in both expression and synchronization of crayfish circadian rhythm, it is not surprising that the kinetics of recovery from light-induce desensitization depends on circadian time (CT). 1,2,3 The slow latency of current onset, as well as the slower CT-dependent recovery from desensitization phase clearly indicate that complex, not yet well determined, biochemical events regulate the light-triggered current, as it has been demonstrated to occur in mammals. 4,5,6,7,8 It is known that biological process that involve complex biochemical cascades can show hysteresis.…”

Section: Introductionmentioning

confidence: 96%

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Electrical response hysteresis of crayfish photoreceptors to light intensity. Dependence on circadian time.

Barriga-Montoya

Gómez-Lagunas

2020

MRAJ

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We studied the light-triggered current of crayfish photoreceptors. We found that when a train of light flashes of either increasing or decreasing intensity is applied, the current waveform presents the non-linear behavior known as hysteresis. Additionally, we observed that the extent of this response depends on the circadian time at which the pulses are applied. We hypothesize that positive feedback loops of biochemical networks underlying light energy transduction are responsible of the observed behavior. It has been demonstrated that a dynamical system hysteresis provides a mechanism that enhances its robustness against random perturbations. Taking into account this characteristic we hypothesize that the electrical-response hysteresis of crayfish photoreceptors: 1) makes the visual system more stable to environmental noise, and hence 2) adds stability to circadian clock oscillations.

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Section: Kinetics Of Crayfish Photoreceptor: Light Intensity Dependencesupporting

confidence: 70%

Section: Kinetics Of Crayfish Photoreceptor: Light Intensity Dependencementioning

confidence: 88%

Section: Kinetics Of Crayfish Photoreceptor: Light Intensity Dependencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Electrical response hysteresis of crayfish photoreceptors to light intensity. Dependence on circadian time.

Barriga-Montoya

Gómez-Lagunas

2020

MRAJ

Self Cite

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“…Some studies have proven that photoperiods can influence the Ca 2+ concentration [36], and thereby influence the growth and survival of cells [37]. In crayfish, Ca 2+ is related to photoreceptor sensitivity has been confirmed [15]. And TRP can be activated by Ca 2+ , to further participate in the process of phototransduction [38, 39].…”

Section: Discussionmentioning

confidence: 99%

The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods

Pang

Zhang

et al. 2019

PLoS ONE

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Photoperiod plays an important role in individual growth, development, and metabolism in crustaceans. The growth and reproduction of crabs are closely related to the photoperiod. However, as of yet, there are still no transcriptomic reports of eyestalk ganglions treated under different photoperiods in the Chinese mitten crab (Eriocheir sinensis), which is a benthonic crab with high commercial value in Asia. In this study, we collected the eyestalk ganglions of crabs that were reared under different photoperiods, including a control group (L: D = 12 h: 12 h, named CC), a constant light group (L: D = 24 h: 0 h, named LL) and a constant darkness group (L: D = 0 h: 24 h, named DD). RNA sequencing was performed on these tissues in order to examine the effects of different photoperiods. The total numbers of clean reads from the CC, LL and DD groups were 48,772,584 bp, 53,943,281 bp and 53,815,178 bp, respectively. After de novo assembly, 161,380 unigenes were obtained and were matched with different databases. The DEGs were significantly enriched in phototransduction and energy metabolism pathways. Results from RT-qPCR showed that TRP channel protein (TRP) in the phototransduction pathway had a significantly higher level of expression in LL and DD groups than in the CC group. We found that the downregulation of the pyruvate dehydrogenase complex (PDC) gene and the upregulation phosphoenolpyruvate carboxykinase (PPC) gene were involved in energy metabolism processes in LL or DD. In addition, we also found that the upregulation of the expression level of the genes Gαq, pyruvate kinase (PK), NADH peroxidase (NADH) and ATPase is involved in phototransduction and energy metabolism. These results may shed some light on the molecular mechanism underlying the effect of photoperiod in physiological activity of E. sinensis.

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Comparative metabolomic analysis of Chinese mitten crab (Eriocheir sinensis) during the daily cycle

Zhang

et al. 2022

Aquaculture Research

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The circadian rhythm of aquatic animals has been a subject of research in a diverse range of studies. However, research on the circadian rhythm of the economically important crustacean Eriocheir sinensis is scarce although this species exhibits an obvious circadian rhythm. In this study, we applied liquid chromatography–mass spectrometry‐based metabolomics to identify key metabolites in the hepatopancreas of E. sinensis under the 24‐h circadian rhythm. Through comparative analysis of the metabolites obtained at different time points (06:00 vs. 12:00 h, 06:00 vs. 18:00 h, and 06:00 vs. 24:00 h), 431 differentially expressed metabolites were identified and classified. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the differentially expressed metabolites were enriched in some circadian rhythm‐related pathways, especially metabolic pathways related to nutrient digestion and absorption. Moreover, several pathways with high impact factors such as “Intestinal immune network for IgA production” were identified, and their functions were mostly related to immunity. This is the first study to identify the metabolites in the hepatopancreas of a crustacean through metabolomic analysis. It provides novel insights into the circadian rhythm of E. sinensis, which could benefit its culture and further increase its economic value.

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Desensitization and recovery of crayfish photoreceptors. Dependency on circadian time, and pigment-dispersing hormone

Cited by 5 publications

References 57 publications

Electrical response hysteresis of crayfish photoreceptors to light intensity. Dependence on circadian time.

Electrical response hysteresis of crayfish photoreceptors to light intensity. Dependence on circadian time.

The transcriptome sequencing and functional analysis of eyestalk ganglions in Chinese mitten crab (Eriocheir sinensis) treated with different photoperiods

Comparative metabolomic analysis of Chinese mitten crab (Eriocheir sinensis) during the daily cycle

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