Desempenho da matriz híbrida SiO2-quitosana na imobilização da lipase microbiana de Candida rugosa

Simões, Aline Souza; Mori, Rafael Cava; Faria, Raquel de Paula; Castro, Heizir F. de; Mendes, Adriano A.

doi:10.1590/s0100-40422011000100007

Cited by 21 publications

(23 citation statements)

References 25 publications

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“…Souza,19 has wrote that immobilized CALB enzyme onto magnetic nanoparticle lost its activity around 20% after 10 min at 60 ºC and the free enzyme, under the same conditions, lost more than 70% of its initial activity. Simões et al 20 has studied the thermal stability of the free and the immobilized on chitosan by covalent bonding Candida rugosa lipase and at 50 ºC the free lipase showed 20% of the residual activity after 60 min of incubation, while the immobilize lipase kept 60% of the initial activity during the same time condition. In order to be potentially applicable on industrial process, one important parameter to the enzyme is the thermal stability, once a lot of process require temperatures that range 40 till 50 ºC.…”

Section: Thermical Stabilitymentioning

confidence: 99%

Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free

Battiston¹,

Ficanha²,

Levandoski³

et al. 2017

Quim. Nova

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publicado na web em 10/02/2017Candida antarctica lipase B (CALB) is an enzyme able to catalyze chemical reaction, however when it is used as a free enzyme, it cannot be recovered from reaction medium. One of the alternatives is to immobilize the enzymes on a support which allows the maintenance of their catalytic activities. The purpose of this paper was to immobilize the CALB on MCM-48 using the ionic solid [C 16 MI]Cl as structure director. 2 2 CCRD (Central Composite Rotational Design) was proposed to analyze the influence of the variables like enzyme mass (0.059 to 0.341 g) and ionic solid concentration (0.59 to 3.41%) in the enzyme immobilization process to obtain the maximum esterification activity in order to optimize the process. After immobilization, the study results showed that the enzymes exhibited improvement of thermal (40, 60 and 80 ºC) and storage stability (90 days), besides the possibility to reuse of the enzyme up to 10 times, showing residual activity of 50%.

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Section: Thermical Stabilitymentioning

confidence: 99%

Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free

Battiston¹,

Ficanha²,

Levandoski³

et al. 2017

Quim. Nova

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“…However, very few endophytic fungi have been reported as producers of lipase. Different supports and methods have been used to immobilize fungal lipases with results of activity retention varying between 30 and 61% (Kim et al 2006;Simões et al 2011;Liu et al 2011;Zhu and Sun 2012). Chitosan is a biopolymer derived from crustacean skeleton chitin and commonly used as support (Biró et al 2008).…”

Section: Lipasesmentioning

confidence: 99%

Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles

Carneiro

Costa-Silva

Souza

et al. 2014

Braz. arch. biol. technol.

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This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated CAM showed better performance retaining 88.4% of the enzymatic activity, with 68.2% of immobilization efficiency (IE

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“…The fish industry produces a great amount of wasted material, such as fish heads and skin that could be used in food production because of their nutritional value, thus contributing to minimize the environmental impacts (Simões, Mori, Faria, De Castro, & Mendes, 2011). For instance, chitosan and the gelatin can be produced from these wastes (Karim & Bhat, 2009;Ocak, 2012).…”

Section: Introductionmentioning

confidence: 99%

Development and optimization of biodegradable fish gelatin composite film added with buriti oil

Silva

Hernández

Araujo

et al. 2018

CyTA - Journal of Food

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The goal of the present study was to determine, through full 2 3 factorial design, the optimum process conditions to develop a biodegradable film, made from gelatin with buriti oil (BO) as additive, characterizing their physical, mechanical, and antioxidant aspects. The conditions for the optimization were 2.3% of fish gelatin, 30% of BO, and 13.18% of plasticizer. The films produced under this condition presented high antioxidant activity (24.77 µmol de TE/g), mechanical resistance (8.04 MPa), and satisfactory water vapor permeability (WVP; 0.38 × 10 −10 g.m. m −2 . s −1 . Pa −1 ). The film presented low solubility in water, low humidity, and low water activity, contributing to its integrity, without identifiable flaws. The increase in the concentration of BO provided higher antioxidant properties to the films and the reduction in gelatin and glycerol concentrations provided lower WVP, increasing the traction resistance.Desarrollo y optimización de una película compuesta hecha de gelatina de pescado biodegradable con aceite de burití añadido RESUMEN A través de un diseño factorial completo de 2 3 , el presente estudio se propuso determinar las condiciones óptimas para desarrollar una película biodegradable, hecha de gelatina con aceite de burití añadido, y caracterizar sus aspectos físicos, mecánicos y antioxidantes. Se pudo constatar que las condiciones óptimas para este proceso incluyen el uso de 2.3% de gelatina de pescado, 30% de aceite de burití y 13.18% de plastificante. Las películas producidas bajo estas condiciones presentan elevada actividad antioxidante (24.77 µmol de TE/g), resistencia mecánica (8.04 MPa) y WVP satisfactoria (0.38 × 10 −10 g.m. m −2 . s −1 . Pa −1 ). Además, la película mostró tener baja solubilidad en el agua, baja humedad y baja actividad en agua, lo cual contribuye a mantener su integridad, sin que se presenten defectos identificables. Al elevarse la concentración de aceite de burití, las películas acusaron propiedades antioxidantes más elevadas. Una reducción de las concentraciones de gelatina y glicerol produjo una permeabilidad más baja al vapor de agua, aumentando la resistencia a la tracción. ARTICLE HISTORY

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Desempenho da matriz híbrida SiO2-quitosana na imobilização da lipase microbiana de Candida rugosa

Cited by 21 publications

References 25 publications

Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free

Immobilization of lipase on mesoporous molecular sieve MCM-48 obtained using ionic solid as a structure director and esterification reaction on solvent-free

Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles

Development and optimization of biodegradable fish gelatin composite film added with buriti oil

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