Description of viral assemblages associated with the Gorgonia ventalina holobiont

Hewson, Ian; Brown, Julia; Burge, Colleen A.; Couch, Courtney S.; LaBarre, Brenna A.; Mouchka, Morgan E.; Naito, Mizue; Harvell, C. Drew

doi:10.1007/s00338-011-0864-x

Cited by 24 publications

(23 citation statements)

References 31 publications

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“…Of these, between 0.06 and 88.03% matched viral genomes, with the remaining sequences corresponding to bacterial, archaeal, or eukaryotic genome fragments. This is similar to the results of previous studies that utilized similar viral genome isolation protocols, and likely results from DNA released from host, bacterial, and/or archaeal cells during the tissue homogenization process (Hewson et al, 2011a(Hewson et al, , 2013Gudenkauf et al, 2014). The low number of viral annotations may be a result of lack of representative viral genomes in public databases (Weynberg et al, 2014).…”

Section: Metagenomic Annotationsupporting

confidence: 78%

“…Because benthic invertebrates play important roles in marine habitat ecology and biogeochemistry, investigation into the viral diversity of their microbiomes is relevant to understanding how viruses influence marine ecosystems. There have been few reports of viruses of echinoderms Hewson et al, 2014), very few of non-commercially important (but ecologically important) marine arthropods (e.g., Dunlap et al, 2013), only one of viruses infecting non-scleractinian corals (Hewson et al, 2011a), and no previous reports of viruses inhabiting urochordates.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Metagenomics of Viral Assemblages Inhabiting Four Phyla of Marine Invertebrates

Gudenkauf

Hewson

2016

Front. Mar. Sci.

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Viruses are the most abundant biological entities on Earth, killing 10-20% of oceanic biomass each day. However, despite their ecological importance, viruses inhabiting many echinoderms, cnidarians, urochordates, and marine arthropods have not been investigated with significant breadth. We conducted a broad survey of the viral assemblages inhabiting these hosts through viral metagenomics and phylogenetic analysis. Results indicate that different invertebrate groups harbor distinct viral assemblages. Interestingly, however, no significant difference is observed between the viral assemblages of echinoderms and arthropods. These similarities and differences may be due to cellular, immunological, geographical, and ecological differences amongst host phyla, although mechanistic determination is beyond the purview of this work. Additionally, we present evidence of the detection of several viral families that have not yet been observed in these hosts. Finally, we confirm the result of previous investigation that method of library construction significantly biases metagenomic results by altering the representation of ssDNA and dsDNA viral genomes.

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Section: Metagenomic Annotationsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Comparative Metagenomics of Viral Assemblages Inhabiting Four Phyla of Marine Invertebrates

Gudenkauf

Hewson

2016

Front. Mar. Sci.

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“…Therefore, local anthropogenic impacts do not seem to be the cause for the bacterial shifts simultaneously observed in the 3 sites in summer 2007, although a monitoring of environmental parameters and microbial community structure in the water column would be required to better evaluate differences between the sites. Finally, the factors that could have directly or indirectly caused a transient change in the natural bacterial community remain unclear and several other hypotheses cannot be ruled out, such as a bacteriophage infection targeting Hahellaceae and causing microbial mortality [66], or the occurrence of subtle alterations in gorgonian physiology in summer [67] that would disrupt the host-bacteria relationship without macroscopic disease signs.…”

Section: Discussionmentioning

confidence: 99%

Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea

et al. 2013

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BackgroundBacterial communities that are associated with tropical reef-forming corals are being increasingly recognized for their role in host physiology and health. However, little is known about the microbial diversity of the communities associated with temperate gorgonian corals, even though these communities are key structural components of the ecosystem. In the Northwestern Mediterranean Sea, gorgonians undergo recurrent mass mortalities, but the potential relationship between these events and the structure of the associated bacterial communities remains unexplored. Because microbial assemblages may contribute to the overall health and disease resistance of their host, a detailed baseline of the associated bacterial diversity is required to better understand the functioning of the gorgonian holobiont.Methodology/Principal FindingsThe bacterial diversity associated with the gorgonian Paramuricea clavata was determined using denaturing gradient gel electrophoresis, terminal-restriction fragment length polymorphism and the construction of clone libraries of the bacterial 16S ribosomal DNA. Three study sites were monitored for 4 years to assess the variability of communities associated with healthy colonies. Bacterial assemblages were highly dominated by one Hahellaceae-related ribotype and exhibited low diversity. While this pattern was mostly conserved through space and time, in summer 2007, a deep shift in microbiota structure toward increased bacterial diversity and the transient disappearance of Hahellaceae was observed.Conclusion/SignificanceThis is the first spatiotemporal study to investigate the bacterial diversity associated with a temperate shallow gorgonian. Our data revealed an established relationship between P. clavata and a specific bacterial group within the Oceanospirillales. These results suggest a potential symbiotic role of Hahellaceae in the host-microbe association, as recently suggested for tropical corals. However, a transient imbalance in bacterial associations can be tolerated by the holobiont without apparent symptoms of disease. The subsequent restoration of the Hahellaceae-dominated community is indicative of the specificity and resilience of the bacteria associated with the gorgonian host.

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“…Only a few studies have reported the metagenomic identification of novel viruses in P. monodon, in Farfantepenaeus duorarum, in copepods and in bivalves [7,[14][15][16][17]. Apart from these economically important species, few studies were published concerning the viromes of ecological important marine invertebrates, such as ghost and volcano shrimps, tunicates, non-scleractinian corals, amphipods and echinoderms [18][19][20][21][22]. However, recent comprehensive surveys have been conducted to discover the genomic diversity of multiple DNA and RNA viruses in arthropods, including marine species [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Exploration of the virome of the European brown shrimp (Crangon crangon)

Eynde

Christiaens

Delbare

et al. 2020

Journal of General Virology

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Crangon crangon is economically a very important species. Recently, promising culture attempts have been made, but a major problem is the uncontrollable mortality during the grow-out phase. As of yet, the life cycle of C. crangon is not closed in captivity so wild-caught individuals are used for further rearing. Therefore, it is important to investigate the virome of C. crangon both in wild-caught animals as in cultured animals. In recent years, next-generation-sequencing (NGS) technologies have been very important in the unravelling of the virome of a wide range of environments and matrices, such as soil, sea, potable water, but also of a wide range of animal species. This will be the first report of a virome study in C. crangon using NGS in combination with the NetoVIR protocol. The near complete genomes of 16 novel viruses were described, most of which were rather distantly related to unclassified viruses or viruses belonging to the Picornavirales, Bunyavirales Nudiviridae, Parvoviridae, Flaviviridae, Hepeviridae, Tombusviridae, Narnaviridae, Nodaviridae, Sobemovirus. A difference in virome composition was observed between muscle and hepatopancreatic tissue, suggesting a distinct tissue tropism of several of these viruses. Some differences in the viral composition were noted between the cultured and wild shrimp, which could indicate that in sub-optimal aquaculture conditions some viruses become more abundant. This research showed that a plethora of unknown viruses is present in C. crangon and that more research is needed to determine which virus is potentially dangerous for the culture of C. crangon.

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Description of viral assemblages associated with the Gorgonia ventalina holobiont

Cited by 24 publications

References 31 publications

Comparative Metagenomics of Viral Assemblages Inhabiting Four Phyla of Marine Invertebrates

Comparative Metagenomics of Viral Assemblages Inhabiting Four Phyla of Marine Invertebrates

Transient Shifts in Bacterial Communities Associated with the Temperate Gorgonian Paramuricea clavata in the Northwestern Mediterranean Sea

Exploration of the virome of the European brown shrimp (Crangon crangon)

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