Description of vancomycin resistance genes in Enterococcus sp. clinical strains isolated from Bucharest, Romania

ALMAHDAWY, OTHMAN THAMER; PRICOP, ROXANA; Sadik, Omar; Najee, Haifaa Bawie; Pîrcălăbioru, Grațiela Grădișteanu; Măruţescu, Luminiţa; Barbu, Ilda Czobor; Banu, Otilia; Cristea, Violeta Corina; Grigore, Raluca; Gheorghe, Irina; Mihăescu, Grigore

doi:10.25083/rbl/24.3/395.399

Cited by 9 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found that the prevalence of vancomycin resistant E. faecium strains was higher than that of E. faecalis, which is similar with the results of another study [14] that reported that out of 23 VRE strains isolated from different clinical specimens at the same hospital in Bucharest, Romania, 18 strains were identified as E. faecium and 5 were E. faecalis. 16 E. faecium strains carried the vanA genotype and 2 strains had the vanB genotype.…”

Section: Prevalence Of Vancomycin Resistance Phenotypes Among Enterocsupporting

confidence: 90%

Prevalence of vancomycin resistance phenotypes among Enterococcus species isolated from clinical samples in a Romanian hospital

2019

Biointerface Res Appl Chem

View full text Add to dashboard Cite

Vancomycin resistant Enterococcus (VRE) has been increasingly reported since the 1980s. Although normally regarded as harmless commensals, enterococci can become the etiological agents of nosocomial infections causing urinary tract infections, peritonitis, bacteriemia, wound infections and endocarditis. The acquisition of vancomycin resistance has seriously affected the treatment and infection control of these bacteria. VRE are frequently resistant to most antibiotics that are effective against vancomycin susceptible enterococci, the therapeutic options of VRE infections being thus very limited. The aim of this study was to assess the genetic support of vancomycin resistance among VRE strains isolated from patients admitted to intensive care units in one hospital unit from Bucharest, Romania Out of 84 enterococcal strains isolated from urine prelevated from catheterized and non-catheterized patients with urinary tract infections, wound secretions, pus, blood, ascite liquid and stool, 28 strains had glycopeptide resistance. Out of these, in the 12 vancomycin-resistant Enterococcus faecalis strains, the VanA phenotype was detected in 9 strains and VanB in 3 strains. From the 16 E. faecium strains resistant to vancomycin, 10 strains showed the VanA phenotype and 6 strains had the VanB phenotype. According to the results of this study, the VanA phenotype, conferring high-level resistance to vancomycin and teicoplanin, was more prevalent than VanB in the Enterococcus sp. strains isolated from hospitalized patients.

show abstract

Section: Prevalence Of Vancomycin Resistance Phenotypes Among Enterocsupporting

confidence: 90%

Prevalence of vancomycin resistance phenotypes among Enterococcus species isolated from clinical samples in a Romanian hospital

2019

Biointerface Res Appl Chem

View full text Add to dashboard Cite

show abstract

“…In order to investigate the genetic support of β-lactam enzymatic resistance (carbapenemases, ESBLs), bacterial DNA was extracted using an adapted alkaline extraction method ( Almahdawy et al, 2019 ). The genetic background of AR was investigated by PCR (PCR Master Mix 2X, Thermo Scientific), using 1 μl of DNA and specific primers for bla TEM ( Quinteros et al, 2003 ), bla SHV ( Kim and Lee, 2000 ), bla CTX–M ( Israil et al, 2013 ), bla OXA– 48 ( Poirel et al, 2011 ), bla VIM ( Shirani et al, 2016 ), bla IMP ( Shirani et al, 2016 ), bla NDM ( Nordmann et al, 2011 ), bla SIM ( Qi et al, 2008 ), bla SPM ( Ellington et al, 2007 ), and bla KPC ( Poirel et al, 2011 ) genes ( Supplementary Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

Multidrug Resistant Klebsiella pneumoniae ST101 Clone Survival Chain From Inpatients to Hospital Effluent After Chlorine Treatment

et al. 2021

Self Cite

View full text Add to dashboard Cite

In this paper we describe the transmission of a multi-drug resistant Klebsiella pneumoniae ST101 clone from hospital to wastewater and its persistence after chlorine treatment. Water samples from influents and effluents of the sewage tank of an infectious diseases hospital and clinical strains collected from the intra-hospital infections, during a period of 10 days prior to wastewater sampling were analyzed. Antibiotic resistant K. pneumoniae strains from wastewaters were recovered on selective media. Based on antibiotic susceptibility profiles and PCR analyses of antibiotic resistance (AR) genetic background, as well as whole-genome sequencing (Illumina MiSeq) and subsequent bioinformatic analyses, 11 ST101 K. pneumoniae strains isolated from hospital wastewater influent, wastewater effluent and clinical sector were identified as clonally related. The SNP and core genome analyses pointed out that five strains were found to be closely related (with ≤18 SNPs and identical cgMLST profile). The strains belonging to this clone harbored multiple acquired AR genes [blaCTX–M–15, blaOXA–48, blaOXA–1, blaSHV–106, blaTEM–150, aac(3)-IIa, aac(6′)-Ib-cr, oqxA10, oqxB17, fosA, catB3, dfrA14, tet(D)] and chromosomal mutations involved in AR (ΔmgrB, ΔompK35, amino acid substitutions in GyrA Ser83Tyr, Asp87Asn, ParC Ser80Tyr). Twenty-nine virulence genes involved in iron acquisition, biofilm and pili formation, adherence, and the type six secretion system – T6SS-III were identified. Our study proves the transmission of MDR K. pneumoniae from hospital to the hospital effluent and its persistence after the chlorine treatment, raising the risk of surface water contamination and further dissemination to different components of the trophic chain, including humans.

show abstract

“…Recent studies demonstrate that a significant proportion of antibiotic-resistant genes in bacteria can be attributed to genetics. The transfer of genetic material between bacteria, via transformation and transduction, is considered the main factor responsible for antibiotic resistance in the majority of bacterial strains [134][135][136].…”

Section: Discussionmentioning

confidence: 99%

Bridging Molecular and Clinical Sciences to Achieve the Best Treatment of Enterococcus faecalis Endocarditis

Nappi,

Avtaar Singh,

Jitendra

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Enterococcus faecalis (E. faecalis) is a commensal bacterium that causes various infections in surgical sites, the urinary tract, and blood. The bacterium is becoming a significant concern because it tends to affect the elderly population, which has a high prevalence of undiagnosed degenerative valvular disease and is often subjected to invasive procedures and implanted medical devices. The bacterium’s actions are influenced by specific characteristics like pili activity and biofilm formation. This resistance significantly impedes the effectiveness of numerous antibiotic therapies, particularly in cases of endocarditis. While current guidelines recommend antimicrobial therapy, the emergence of resistant strains has introduced complexity in managing these patients, especially with the increasing use of transcatheter therapies for those who are not suitable for surgery. Presentations of the condition are often varied and associated with generalised symptoms, which may pose a diagnostic challenge. We share our encounter with a case study that concerns an octogenarian who had a TAVI valve and developed endocarditis. We also conducted a literature review to identify the essential treatment algorithms for such cases.

show abstract

Description of vancomycin resistance genes in Enterococcus sp. clinical strains isolated from Bucharest, Romania

Cited by 9 publications

References 8 publications

Prevalence of vancomycin resistance phenotypes among Enterococcus species isolated from clinical samples in a Romanian hospital

Prevalence of vancomycin resistance phenotypes among Enterococcus species isolated from clinical samples in a Romanian hospital

Multidrug Resistant Klebsiella pneumoniae ST101 Clone Survival Chain From Inpatients to Hospital Effluent After Chlorine Treatment

Bridging Molecular and Clinical Sciences to Achieve the Best Treatment of Enterococcus faecalis Endocarditis

Contact Info

Product

Resources

About