Description of Third Instars of &lt;I&gt;Cochliomyia minima&lt;/I&gt; (Diptera: Calliphoridae) From West Indies, and Updated Identification Key

Yusseff-Vanegas, Sohath Z

doi:10.1603/me13088

Cited by 9 publications

(7 citation statements)

References 10 publications

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“…In recent studies of Cochliomyia minima in Puerto Rico, Yusseff-Vanegas (2014) reported that Cochliomyia minima prefer highly humid areas and can tolerate relatively cool conditions at altitudes >800m, while this species is absent from extremely dry and hot areas. Similar results were found in Dominican Republic and Cuba where Cochliomyia minima was found abundantly in tropical and subtropical rain/moist forest even at altitudes >1300m, but absent from dry forest (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

“…Yet, due to their abundance and broad distribution in this region, including Cuba, Dominican Republic, Jamaica, Puerto Rico, Virgin Islands, Bahamas and Cayman Islands (Hall 1948, Dear 1985, Whitworth 2010) they have an enormous forensic potential. For example, recent studies conducted in Puerto Rico showed that Cochliomyia minima is abundant and widely distributed on the island, and that adults are attracted to, and feed on, carrion (Yusseff-Vanegas 2014). …”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, DNA-based methods can provide reliable identification of specimens by non-experts and will be particularly important for the identification of larval stages of Cochliomyia minina and Cochliomyia aldrichi that remain poorly known. For example, only the third instar of Cochliomyia minima has been described (Yusseff-Vanegas 2014). …”

Section: Introductionmentioning

confidence: 99%

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Molecular phylogeny of the forensically important genus Cochliomyia (Diptera: Calliphoridae)

Yusseff-Vanegas¹,

Agnarsson²

2016

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Cochliomyia Townsend includes several abundant and one of the most broadly distributed, blow flies in the Americas, and is of significant economic and forensic importance. For decades, Cochliomyia hominivorax (Coquerel) and Cochliomyia macellaria (Fabricius) have received attention as livestock parasites and primary indicator species in forensic entomology. However, Cochliomyia minima Shannon and Cochliomyia aldrichi Del Ponte have only been subject to basic taxonomy and faunistic studies. Here we present the first complete phylogeny of Cochliomyia including numerous specimens per species, collected from 13 localities in the Caribbean. Four genes, the mitochondrial COI and the nuclear EF-1α, 28S rRNA, and ITS2, were analyzed. While we found some differences among gene trees, a concatenated gene matrix recovered a robustly supported monophyletic Cochliomyia with Compsomyiops Townsend as its sister group and recovered the monophyly of Cochliomyia hominivorax, Cochliomyia macellaria and Cochliomyia minima. Our results support a close relationship between Cochliomyia minima and Cochliomyia aldrichi. However, we found Cochliomyia aldrichi containing Cochliomyia minima, indicating recent speciation, or issues with the taxonomy of the group. We provide basic information on habitat preference, distribution and feeding habits of Cochliomyia minima and Cochliomyia aldrichi that will be useful for future forensic studies in the Caribbean.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular phylogeny of the forensically important genus Cochliomyia (Diptera: Calliphoridae)

Yusseff-Vanegas¹,

Agnarsson²

2016

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“…Preservation of a portion of the specimens was accomplished by hot water kill and placement in 70% ethanol [12,17] and the remainder of the specimens were reared to confirm identification. Identification of the larvae was accomplished with the use of taxon and life stage appropriate keys (e.g., [18,19,20,21,22]).…”

Section: Methodsmentioning

confidence: 99%

Comparing Species Composition of Passive Trapping of Adult Flies with Larval Collections from the Body during Scene-Based Medicolegal Death Investigations

Sanford

2017

Insects

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Collection of insects at the scene is one of the most important aspects of forensic entomology and proper collection is one of the biggest challenges for any investigator. Adult flies are highly mobile and ubiquitous at scenes, yet their link to the body and the time of colonization (TOC) and post-mortem interval (PMI) estimates is not well established. Collection of adults is widely recommended for casework but has yet to be rigorously evaluated during medicolegal death investigations for its value to the investigation. In this study, sticky card traps and immature collections were compared for 22 cases investigated by the Harris County Institute of Forensic Sciences, Houston, TX, USA. Cases included all manner of death classifications and a range of decomposition stages from indoor and outdoor scenes. Overall, the two methods successfully collected at least one species in common only 65% of the time, with at least one species unique to one of the methods 95% of the time. These results suggest that rearing of immature specimens collected from the body should be emphasized during training to ensure specimens directly associated with the colonization of the body can be identified using adult stages if necessary.

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“…Here, we show DNA barcoding to be useful in overcoming these problems and provide tools to accelerate the identification and discovery of species. This is particularly important in areas like the Caribbean, where studies of insects involved in cadaveric decomposition are scarce (Whitworth, 2010;Yusseff-Vanegas & Agnarsson, 2016;Yusseff-Vanegas, 2007;Yusseff-Vanegas, 2014). One of the first steps required for this approach is creating a reliable DNA barcode database that can be used with confidence in order to identify unknown specimens found in death scenes investigation (DeBry et al, 2013;Harvey et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

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Supplemental Information 2: Phylogenetic Relationship Within Calliphoridae Based on a Bayesian Analysis of Nucleotide Data From

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6Correct identification of forensically important insects, such as flies in the family Calliphoridae, 7 is a crucial step for them to be used as evidence in legal investigations. Traditional identification 8 based on morphology has been effective, but has some limitations when it comes to identify 9 immature stages of certain species. DNA-barcoding, using COI, has demonstrated potential for 10 rapid and accurate identification of Calliphoridae, however, this gene does not reliably

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Description of Third Instars of <I>Cochliomyia minima</I> (Diptera: Calliphoridae) From West Indies, and Updated Identification Key

Cited by 9 publications

References 10 publications

Molecular phylogeny of the forensically important genus Cochliomyia (Diptera: Calliphoridae)

Molecular phylogeny of the forensically important genus Cochliomyia (Diptera: Calliphoridae)

Comparing Species Composition of Passive Trapping of Adult Flies with Larval Collections from the Body during Scene-Based Medicolegal Death Investigations

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