Description of the Virus Infection

Oirschot, J.T. van

doi:10.1007/978-1-4613-2083-8_1

Cited by 41 publications

(30 citation statements)

References 36 publications

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“…Pestivirus in the family Flaviviridae and is the causative agent of classical swine fever, the clinical symptoms of which vary from mild to severe depending on the strain causing infection (Summerfield et al, 1998(Summerfield et al, , 2000(Summerfield et al, , 2001van Oirschot, 1988). Pestiviruses such as CSFV and bovine viral diarrhea virus (BVDV) are able to cross the placenta and can establish an infection in the developing fetus, resulting in the birth of persistently infected animals (Charleston et al, 2001).…”

Section: Classical Swine Fever Virus (Csfv) Is a Member Of The Genusmentioning

confidence: 99%

The Npro product of classical swine fever virus interacts with IκBα, the NF-κB inhibitor

Doceul

Charleston

Crooke

et al. 2008

Journal of General Virology

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Classical swine fever virus (CSFV) belongs to the genus Pestivirus and is the causative agent of classical swine fever, a haemorrhagic disease of pigs. The virus replicates in host cells without activating interferon (IFN) production and has been reported to be an antagonist of doublestranded RNA-induced apoptosis. The N-terminal protease (N pro ) of CSFV is responsible for this evasion of the host innate immune response. In order to identify cellular proteins that interact with the N pro product of CSFV, a yeast two-hybrid screen of a human library was carried out, which identified IkBa, the inhibitor of NF-kB, a transcription factor involved in the control of apoptosis, the immune response and IFN production. The N pro -IkBa interaction was confirmed using yeast two-hybrid analysis and additional co-precipitation assays. It was also shown that N pro localizes to both the cytoplasmic and nuclear compartments in stably transfected cells and in CSFVinfected cells. Following stimulation by tumour necrosis factor alpha, PK-15 cell lines expressing N pro exhibited transient nuclear accumulation of pIkBa, but no effect of CSFV infection on IkBa localization or NF-kB p65 activation was observed.

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Section: Classical Swine Fever Virus (Csfv) Is a Member Of The Genusmentioning

confidence: 99%

The Npro product of classical swine fever virus interacts with IκBα, the NF-κB inhibitor

Doceul

Charleston

Crooke

et al. 2008

Journal of General Virology

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“…The causative agent, classical swine fever virus (CSFV), is a small enveloped RNA virus with a genome consisting of a single-stranded RNA of positive polarity (Meyers et al, 1989 ;Moormann et al, 1990) and has been classified into the genus Pestivirus within the family Flaviviridae (Wengler, 1991). Depending on the virulence of the strain, the infection dose and host factors such as age and immunocompetence, infection of pigs with CSFV may lead to peracute, acute, chronic, late onset or clinically inapparent courses of disease (Van Oirschot, 1988).…”

Section: Introductionmentioning

confidence: 99%

Infection with classical swine fever virus: effects on phenotype and immune responsiveness of porcine T lymphocytes.

Pauly

König

Hj³

et al. 1998

Journal of General Virology

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T lymphocytes obtained from pigs infected with a lethal dose of classical swine fever virus were analysed for phenotypic changes in the composition of T-cell subpopulations and for alterations in their immune responsiveness in vitro during the course of disease. Viral antigen detected in all subpopulations and the selective depletion of CD4 N CD8 N γ/δ T cells showed that peripheral blood T lymphocytes were affected in the terminal stage (14-19 days postinfection) of classical swine fever whereas no implications for T lymphocytes were obvious during the

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“…9 The disease is characterized by a peracute, acute, subacute, chronic, atypical, or inapparent course. 27,30,31 Chronic CSF is a lethal disease with a duration of at least 30 days. 18 There is often little evidence of CSF in terms of petechial hemorrhages in most organ systems, but a single organ system (lung, gastrointestinal tract, and central nervous system) may be predominantly affected.…”

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confidence: 99%

“…18 There is often little evidence of CSF in terms of petechial hemorrhages in most organ systems, but a single organ system (lung, gastrointestinal tract, and central nervous system) may be predominantly affected. 30,31 The clinical signs may often be uncharacteristic and misleading; therefore, chronic CSF was erroneously called atypical CSF. 6,27 Brief Communications and Case Reports Chronic CSF has been observed under both natural and experimental conditions.…”

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confidence: 99%

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Localization of Classical Swine Fever Virus from Chronically Infected Pigs by In Situ Hybridization and Immunohistochemistry

Choi¹,

Chae²

2003

Vet Pathol

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Abstract. Classical swine fever (CSF) virus (CSFV) nucleic acid and antigen were detected in 15 pigs with naturally occurring chronic CSF by in situ hybridization and immunohistochemistry. The most consistent and prominent microscopic lesions were perivascular mononuclear cell infiltration and gliosis in the central nervous system of pigs with chronic CSF. Positive cells typically exhibited a dark brown (in situ hybridization) or red (immunohistochemistry) reaction product in the cytoplasm without background staining. A positive signal for both in situ hybridization and immunohistochemistry was detected in mononuclear cells and lymphocytes of lymphoid tissues. Viral nucleic acid was detected in some tissue sections in the absence of viral antigen. The in situ hybridization technique developed in this study was useful for the detection of CSFV RNA in tissues taken from chronically infected pigs and may be a valuable technique for studying the pathogenesis of chronic CSFV infection.

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Description of the Virus Infection

Cited by 41 publications

References 36 publications

The Npro product of classical swine fever virus interacts with IκBα, the NF-κB inhibitor

The Npro product of classical swine fever virus interacts with IκBα, the NF-κB inhibitor

Infection with classical swine fever virus: effects on phenotype and immune responsiveness of porcine T lymphocytes.

Localization of Classical Swine Fever Virus from Chronically Infected Pigs by In Situ Hybridization and Immunohistochemistry

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