Description of Sergentomyia phadangensis n. sp. (Diptera, Psychodidae) of Thailand

Polseela, Raxsina; Depaquit, Jérôme; Apiwathnasorn, Chamnarn

doi:10.1186/s13071-016-1300-4

Cited by 12 publications

(7 citation statements)

References 18 publications

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“…[37][38][39][40][41][42][43][44][45][46][47] A recent review of sandfly distribution in Thailand indicated that at least 27 species of the four genera, Sergentomyia, Phlebotomus, Idiophlebotomus, and Chinius were identified. 42,43 Certain species such as Sergentomyia gemmea are the most predominant species in the north 33 as well as in the south. 24,42,44 The prevalence of S. gemmea was as high as 85-95% in the northern and southern areas.…”

Section: Sandfly Distribution and Potential Vectormentioning

confidence: 99%

Leishmaniasis in Thailand: A Review of Causative Agents and Situations

Leelayoova

Siripattanapipong

Manomat

et al. 2017

The American Society of Tropical Medicine and Hygiene

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Abstract. Before 1999, leishmaniasis was considered an imported disease in Thailand. Since then, autochthonous leishmaniasis was reported in both immmunocompetent and immmunocompromised patients especially in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS). A new species was identified and named as Leishmania siamensis consisting of two lineages, that is, lineages TR and PG. Analysis of isoenzymes has clarified the more commonly detected L. siamensis lineage PG as Leishmania martiniquensis (MON-229), a species originally reported from the Martinique Island, whereas the L. siamensis lineage TR has been identified as the true novel species, L. siamensis (MON-324). Both cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL) have been found among Thai patients. Disseminated CL and VL could be presented in some reported patients who had HIV/AIDS coinfection. So far, only sporadic cases have been reported; thus, the true prevalence of leishmaniasis should be determined in Thailand among the high-risk populations such as people with HIV/AIDS. A recent survey among animals identified L. martiniquensis DNA in black rats (Rattus rattus) suggesting a potential animal reservoir. In addition, L. martiniquensis DNA was identified in Sergentomyia gemmea and Sergentomyia barraudi, the predominant sandfly species in the affected areas. However, further studies are needed to prove that these sandflies could serve as the vector of leishmaniasis in Thailand.

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Section: Sandfly Distribution and Potential Vectormentioning

confidence: 99%

Leishmaniasis in Thailand: A Review of Causative Agents and Situations

Leelayoova

Siripattanapipong

Manomat

et al. 2017

The American Society of Tropical Medicine and Hygiene

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“…In the present study, DNA barcoding analysis was performed to identify the species of the 24 female sand fly specimens that could be classified only to the genus level based on morphological characters; 12 of these 24 specimens were subsequently identified using DNA barcoding to the species level, including P. mascomai , S. bailyi , S. gemmea , S. perturbans and S. rudnicki ; however, the remaining 12 specimens were still identified to the genus level only. These unclassified species may be newly recorded species; however, an analysis using additional DNA markers, such as the Cytb gene [ 16 , 44 ], is warranted prior to reaching a definitive conclusion. The DNA barcoding showed that five (11.4%) of the 44 specimens identified to species level on the basis of morphological chararacters actually belonged to a different species.…”

Section: Discussionmentioning

confidence: 99%

“…Although Thailand is not considered to be an endemic country for leishmaniasis, since 1999 there has been an increase in the number of reported cases of autochthonous leishmaniasis in several regions of the country [ 15 ]. A recent review of sand fly distribution in Thailand indicated that at least 27 species of the genera Sergentomyia , Phlebotomus , Idiophlebotomus and Chinius have been identified [ 16 , 17 ]. Sergentomyia gemmea is the predominant species in Thailand and is considered to be a potential vector of L. orientalis [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Sand fly identification and screening for Leishmania spp. in six provinces of Thailand

et al. 2021

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Background Phlebotomine sand flies are vectors of Leishmania spp. At least 27 species of sand flies have been recorded in Thailand. Although human leishmaniasis cases in Thailand are mainly imported, autochthonous leishmaniasis has been increasingly reported in several regions of the country since 1999. Few studies have detected Leishmania infection in wild-caught sand flies, although these studies were carried out only in those areas reporting human leishmaniasis cases. The aim of this study was therefore to identity sand fly species and to investigate Leishmania infection across six provinces of Thailand. Methods Species of wild-caught sand flies were initially identified based on morphological characters. However, problems identifying cryptic species complexes necessitated molecular identification using DNA barcoding in parallel with identification based on morphological characters. The wild-caught sand flies were pooled and the DNA isolated prior to the detection of Leishmania infection by a TaqMan real-time PCR assay. Results A total of 4498 sand flies (1158 males and 3340 females) were caught by trapping in six provinces in four regions of Thailand. The sand flies were morphologically classified into eight species belonging to three genera (Sergentomyia, Phlebotomus and Idiophlebotomus). Sergentomyia iyengari was found at all collection sites and was the dominant species at most of these, followed in frequency by Sergentomyia barraudi and Phlebotomus stantoni, respectively. DNA barcodes generated from 68 sand flies allowed sorting into 14 distinct species with 25 operational taxonomic units, indicating a higher diversity (by 75%) than that based on morphological identification. Twelve barcoding sequences could not be assigned to any species for which cytochrome c oxidase subunit I sequences are available. All tested sand flies were negative for Leishmania DNA. Conclusions Our results confirm the presence of several sand fly species in different provinces of Thailand, highlighting the importance of using DNA barcoding as a tool to study sand fly species diversity. While all female sand flies tested in this study were negative for Leishmania, the circulation of Leishmania spp. in the investigated areas cannot be ruled out. Graphical abstract

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“…Identi cation of the specimens were done by using Adler and eodor (1926), Raynal (1937Raynal ( ), eodor (1948, Kirk and Lewis (1951), Quate (1962), Lewis (1973Lewis ( , 1978, Leng (1997). Terminology and abbreviations follows Quate (1962) except antennal formulas and thorax, which follows Polseela et al (2016) and Galati et al (2017) respectively. e specimens were studied using BX51 and CH-2 microscopes (Olympus Co., Japan) and measured using the microscopy imaging so ware cellSens standard (Olympus Co., Japan).…”

Section: Specimen Preservation Preparation and Identi Cationmentioning

confidence: 99%